Target Interacting Protein Information

Content Navigation (  All   None )                                      
Target General Information
Target Interacting Proteins
References
Target General Information  Top
Target ID T35445 Target Info
Target Name Mothers against decapentaplegic homolog 3 (SMAD3)
Synonyms TGF-beta response effector Smad3; Smad3; SMAD family member 3; SMAD 3; Mothers against DPP homolog 3; Mad3; MMad3; MADH3; MAD homolog 3; JV15-2; HSMAD3; HMAD-3
Target Type Preclinical Target
Gene Name SMAD3
UniProt ID
SMAD3_HUMAN 
Target Interacting Proteins  Top
Acyltransferases [+] 9 Acyltransferases +
Interacting Target Name Histone acetyltransferase p300 (EP300) Clinical trial Target Interacting Protein Info
UniProt ID
EP300_HUMAN 
Synonyms Protein propionyltransferase p300; p300 HAT; P300; Histone crotonyltransferase p300; Histone butyryltransferase p300; E1A-associated protein p300; E1Aassociated protein p300
Evidence Score (E-score) 10 Experimental Evidences +
1 Affinity Capture-Western [1]
2 Affinity Capture-Western [2]
3 Affinity Capture-Western [3]
4 Affinity Capture-Western [4]
5 Affinity Capture-Western [5]
6 Affinity Capture-Western [6]
7 Reconstituted Complex [7]
8 Reconstituted Complex [8]
9 Reconstituted Complex [5]
10 Reconstituted Complex [9]
Interacting Protein Name E3 ubiquitin-protein ligase SMURF2 (SMURF2) Interacting Protein Interacting Protein Info
UniProt ID
SMUF2_HUMAN 
Evidence Score (E-score) 8 Experimental Evidences +
1 Affinity Capture-Luminescence [10]
2 Affinity Capture-MS [11]
3 Affinity Capture-Western [12]
4 Affinity Capture-Western [13]
5 Affinity Capture-Western [14]
6 Reconstituted Complex [15]
7 Yeast two hybrid [15]
8 Yeast two hybrid [14]
Interacting Target Name Histone acetyltransferase KAT2B (KAT2B) Literature-reported Target Interacting Protein Info
UniProt ID
KAT2B_HUMAN 
Synonyms Spermidine acetyltransferase KAT2B; PCAF; P300/CBP-associated factor; P/CAF; Lysine acetyltransferase 2B; Histone acetyltransferase PCAF; Histone acetylase PCAF
Evidence Score (E-score) 4 Experimental Evidences +
1 Affinity Capture-Western [16]
2 Affinity Capture-Western [17]
3 Affinity Capture-Western [18]
4 Reconstituted Complex [7]
Interacting Target Name E3 ubiquitin-protein ligase NEDD4 (NEDD4) Literature-reported Target Interacting Protein Info
UniProt ID
NEDD4_HUMAN 
Synonyms Neural precursor cell expressed developmentally down-regulated protein 4; NEDD4-1; NEDD-4; KIAA0093; HECT-type E3 ubiquitin transferase NEDD4; Cell proliferation-inducing gene 53 protein
Evidence Score (E-score) 4 Experimental Evidences +
1 Affinity Capture-Western [19]
2 Affinity Capture-Western [20]
3 Protein-peptide [20]
4 Reconstituted Complex [20]
Interacting Protein Name E3 ubiquitin-protein ligase NEDD4-like (NEDD4L) Interacting Protein Interacting Protein Info
UniProt ID
NED4L_HUMAN 
Synonyms NEDD4.2; NEDD4.2; RSP5; hNEDD4.2
Evidence Score (E-score) 4 Experimental Evidences +
1 Affinity Capture-Western [21]
2 Affinity Capture-Western [22]
3 Affinity Capture-Western [23]
4 Reconstituted Complex [22]
Interacting Protein Name Breast cancer type 1 susceptibility protein (BRCA1) Interacting Protein Interacting Protein Info
UniProt ID
BRCA1_HUMAN 
Synonyms BRCAI; BRCC1; BROVCA1; FANCS; IRIS; PNCA4; PPP1R53; PSCP; RNF53
Evidence Score (E-score) 3 Experimental Evidences +
1 Affinity Capture-Western [24]
2 Affinity Capture-Western [25]
3 Reconstituted Complex [24]
Interacting Protein Name E3 ubiquitin-protein ligase TRIM33 (TRIM33) Interacting Protein Interacting Protein Info
UniProt ID
TRI33_HUMAN 
Synonyms ECTO; PTC7; RFG7; TF1G; TIF1G; TIF1GAMMA; TIFGAMMA
Evidence Score (E-score) 3 Experimental Evidences +
1 Affinity Capture-MS [26]
2 Affinity Capture-Western [27]
3 Reconstituted Complex [22]
Interacting Protein Name E3 ubiquitin-protein ligase SMURF1 (SMURF1) Interacting Protein Interacting Protein Info
UniProt ID
SMUF1_HUMAN 
Evidence Score (E-score) 3 Experimental Evidences +
1 Affinity Capture-Western [12]
2 Affinity Capture-Western [28]
3 Yeast two hybrid [29]
Interacting Protein Name WW domain-containing protein 1 (WWP1) Interacting Protein Interacting Protein Info
UniProt ID
WWP1_HUMAN 
Synonyms WW domain-containing protein 1; Tiul1; TGIF-interacting ubiquitin ligase 1; NEDD4-like E3 ubiquitin-protein ligase WWP1; HECT-type E3 ubiquitin transferase WWP1; Atrophin-1-interacting protein 5; AIP5
Evidence Score (E-score) 3 Experimental Evidences +
1 Affinity Capture-Western [12]
2 Yeast two hybrid [30]
3 Yeast two hybrid [31]
Adapter proteins [+] 1 Adapter proteins +
Interacting Protein Name Erbin (ERBIN) Interacting Protein Interacting Protein Info
UniProt ID
ERBIN_HUMAN 
Synonyms ERBIN; HEL.S.78; LAP2
Evidence Score (E-score) 5 Experimental Evidences +
1 Affinity Capture-MS [26]
2 Affinity Capture-MS [11]
3 Affinity Capture-Western [32]
4 Reconstituted Complex [33]
5 Reconstituted Complex [32]
Carbon-nitrogen hydrolases [+] 1 Carbon-nitrogen hydrolases +
Interacting Target Name Histone deacetylase 1 (HDAC1) Successful Target Interacting Protein Info
UniProt ID
HDAC1_HUMAN 
Synonyms RPD3L1; HD1
Evidence Score (E-score) 2 Experimental Evidences +
1 Reconstituted Complex [9]
2 Yeast two hybrid [34]
Isomerases [+] 1 Isomerases +
Interacting Target Name Rotamase Pin1 (PIN1) Clinical trial Target Interacting Protein Info
UniProt ID
PIN1_HUMAN 
Synonyms Prolyl isomerase Pin1; PPIase Pin1; Peptidyl-prolyl cis-trans isomerase Pin1; Peptidyl-prolyl cis-trans isomerase NIMA-interacting 1; Peptidyl prolyl cis/trans isomerase Pin1
Evidence Score (E-score) 3 Experimental Evidences +
1 Affinity Capture-Western [35]
2 Affinity Capture-Western [36]
3 Protein-peptide [20]
Kinases [+] 3 Kinases +
Interacting Target Name Cyclin-dependent kinase 4 (CDK4) Successful Target Interacting Protein Info
UniProt ID
CDK4_HUMAN 
Synonyms PSK-J3; Cell division protein kinase 4
Evidence Score (E-score) 2 Experimental Evidences +
1 Biochemical Activity [37]
2 Co-localization [38]
Interacting Target Name Cyclin-dependent kinase 2 (CDK2) Clinical trial Target Interacting Protein Info
UniProt ID
CDK2_HUMAN 
Synonyms SIN3-associated protein; Sin3 associated polypeptide; P33 protein kinase; Cell division protein kinase 2; CDKN2
Evidence Score (E-score) 2 Experimental Evidences +
1 Biochemical Activity [37]
2 Co-localization [38]
Interacting Target Name TGF-beta receptor type I (TGFBR1) Clinical trial Target Interacting Protein Info
UniProt ID
TGFR1_HUMAN 
Synonyms Type I TGFbeta receptor kinase; Transforming growth factor-beta receptor type I; TGFR-1; TGF-beta type I receptor; TGF-beta receptor type-1; TbetaR-I; TbetaRI; SKR4; Serine/threonine-protein kinase receptor R4; ALK-5; ALK5; Activin receptor-like kinase 5; Activin A receptor type II-like protein kinase of 53kD
Evidence Score (E-score) 2 Experimental Evidences +
1 Affinity Capture-Western [39]
2 Biochemical Activity [40]
Nuclear hormone receptors [+] 1 Nuclear hormone receptors +
Interacting Target Name Androgen receptor (AR) Successful Target Interacting Protein Info
UniProt ID
ANDR_HUMAN 
Synonyms Testosterone receptor; Nuclear receptor subfamily 3 group C member 4; NR3C4; Dihydrotestosterone receptor; DHTR; Androgen receptor
Evidence Score (E-score) 6 Experimental Evidences +
1 Affinity Capture-Western [41]
2 Affinity Capture-Western [42]
3 Affinity Capture-Western [43]
4 Reconstituted Complex [41]
5 Reconstituted Complex [42]
6 Reconstituted Complex [43]
Peptidases [+] 3 Peptidases +
Interacting Protein Name Ubiquitin carboxyl-terminal hydrolase 15 (USP15) Interacting Protein Interacting Protein Info
UniProt ID
UBP15_HUMAN 
Synonyms UNPH.2; UNPH4
Evidence Score (E-score) 3 Experimental Evidences +
1 Affinity Capture-Western [44]
2 Affinity Capture-Western [45]
3 Reconstituted Complex [44]
Interacting Target Name Ubiquitin C-terminal hydrolase UCH37 (UCHL5) Patented-recorded Target Interacting Protein Info
UniProt ID
UCHL5_HUMAN 
Synonyms UCH-L5; UCH37; Ubiquitin thioesterase L5; Ubiquitin carboxyl-terminal hydrolase isozyme L5; CGI-70; AD-019
Evidence Score (E-score) 2 Experimental Evidences +
1 Affinity Capture-Western [46]
2 Reconstituted Complex [47]
Interacting Target Name Herpesvirus ubiquitin-specific protease (HAUSP) Preclinical Target Interacting Protein Info
UniProt ID
UBP7_HUMAN 
Synonyms Ubiquitin-specific-processing protease 7; Ubiquitin thioesterase 7; Ubiquitin carboxyl-terminal hydrolase 7; Herpesvirus-associated ubiquitin-specific protease; Deubiquitinating enzyme 7
Evidence Score (E-score) 2 Experimental Evidences +
1 Affinity Capture-MS [11]
2 Yeast two hybrid [48]
Pore-forming toxins [+] 1 Pore-forming toxins +
Interacting Target Name Cellular tumor antigen p53 (TP53) Clinical trial Target Interacting Protein Info
UniProt ID
P53_HUMAN 
Synonyms Tumor suppressor p53; Phosphoprotein p53; P53; Antigen NY-CO-13
Evidence Score (E-score) 3 Experimental Evidences +
1 Affinity Capture-Western [49]
2 Co-localization [50]
3 Reconstituted Complex [49]
Transcription regulators [+] 15 Transcription regulators +
Interacting Protein Name Mothers against decapentaplegic homolog 4 (SMAD4) Interacting Protein Interacting Protein Info
UniProt ID
SMAD4_HUMAN 
Synonyms DPC4; JIP; MADH4; MYHRS
Evidence Score (E-score) 20 Experimental Evidences +
1 Affinity Capture-MS [11]
2 Affinity Capture-Western [51]
3 Affinity Capture-Western [52]
4 Affinity Capture-Western [22]
5 Affinity Capture-Western [23]
6 Affinity Capture-Western [25]
7 Affinity Capture-Western [4]
8 Affinity Capture-Western [11]
9 Affinity Capture-Western [53]
10 Affinity Capture-Western [54]
11 Affinity Capture-Western [39]
12 Affinity Capture-Western [55]
13 Affinity Capture-Western [56]
14 Affinity Capture-Western [57]
15 Affinity Capture-Western [58]
16 Affinity Capture-Western [59]
17 Affinity Capture-Western [60]
18 Affinity Capture-Western [61]
19 Affinity Capture-Western [62]
20 Affinity Capture-Western [63]
Interacting Protein Name Ski oncogene (SKI) Interacting Protein Interacting Protein Info
UniProt ID
SKI_HUMAN 
Synonyms SGS; SKV
Evidence Score (E-score) 9 Experimental Evidences +
1 Affinity Capture-Western [64]
2 Affinity Capture-Western [65]
3 Affinity Capture-Western [6]
4 Reconstituted Complex [66]
5 Reconstituted Complex [67]
6 Reconstituted Complex [9]
7 Reconstituted Complex [34]
8 Reconstituted Complex [68]
9 Reconstituted Complex [69]
Interacting Target Name CREB-binding protein (CREBBP) Clinical trial Target Interacting Protein Info
UniProt ID
CBP_HUMAN 
Synonyms CREBbinding protein; CBP
Evidence Score (E-score) 8 Experimental Evidences +
1 Affinity Capture-MS [26]
2 Affinity Capture-Western [70]
3 Affinity Capture-Western [71]
4 Affinity Capture-Western [72]
5 Affinity Capture-Western [73]
6 Protein-peptide [73]
7 Reconstituted Complex [73]
8 Reconstituted Complex [67]
Interacting Protein Name Mothers against decapentaplegic homolog 2 (SMAD2) Interacting Protein Interacting Protein Info
UniProt ID
SMAD2_HUMAN 
Synonyms JV18; JV18.1; MADH2; MADR2; hMAD.2; hSMAD2
Evidence Score (E-score) 7 Experimental Evidences +
1 Affinity Capture-MS [74]
2 Affinity Capture-MS [75]
3 Affinity Capture-MS [11]
4 Affinity Capture-Western [39]
5 Affinity Capture-Western [54]
6 Affinity Capture-Western [55]
7 Yeast two hybrid [76]
Interacting Target Name Transcription factor AP-1 (JUN) Discontinued Target Interacting Protein Info
UniProt ID
JUN_HUMAN 
Synonyms V-jun avian sarcoma virus 17 oncogene homolog; Transcription factor AP-1; Proto-oncogene c-jun; P39; C-jun proto-oncogene; AP-1 transcription factor; AP1; Activator protein-1; Activator protein 1
Evidence Score (E-score) 6 Experimental Evidences +
1 Affinity Capture-Western [77]
2 Affinity Capture-Western [78]
3 Reconstituted Complex [77]
4 Reconstituted Complex [34]
5 Reconstituted Complex [79]
6 Yeast two hybrid [77]
Interacting Target Name Hypoxia-inducible factor 1 alpha (HIF-1A) Clinical trial Target Interacting Protein Info
UniProt ID
HIF1A_HUMAN 
Synonyms Transcription factor HIF-1; PASD8; PAS domain-containing protein 8; MOP1; Member of PAS protein 1; Hypoxia-inducible transcription factor (HIF)-1; Hypoxia-inducible factor 1-alpha; Hypoxia-inducible factor 1; Hypoxia inducible factor 1; HIF-1-alpha; HIF-1alpha; HIF1-alpha; HIF-1 alpha; HIF1 alpha; Class E basic helix-loop-helix protein 78; bHLHe78; Basic-helix-loop-helix-PAS protein MOP1; ARNT-interacting protein; ARNT interacting protein
Evidence Score (E-score) 5 Experimental Evidences +
1 Affinity Capture-Luminescence [80]
2 Affinity Capture-Western [81]
3 Co-localization [81]
4 Reconstituted Complex [81]
5 Yeast two hybrid [80]
Interacting Protein Name Ski-like protein (SKIL) Interacting Protein Interacting Protein Info
UniProt ID
SKIL_HUMAN 
Synonyms SNO; SnoA; SnoI; SnoN
Evidence Score (E-score) 5 Experimental Evidences +
1 Affinity Capture-Western [82]
2 Affinity Capture-Western [65]
3 Affinity Capture-Western [6]
4 Affinity Capture-Western [83]
5 Reconstituted Complex [34]
Interacting Target Name Transcription factor Sp1 (SP1) Clinical trial Target Interacting Protein Info
UniProt ID
SP1_HUMAN 
Synonyms TSFP1
Evidence Score (E-score) 3 Experimental Evidences +
1 Affinity Capture-Western [84]
2 Affinity Capture-Western [59]
3 Reconstituted Complex [85]
Interacting Target Name Runt-related transcription factor 2 (RUNX2) Literature-reported Target Interacting Protein Info
UniProt ID
RUNX2_HUMAN 
Synonyms SL3-3 enhancer factor 1 alpha A subunit; SL3/AKV core-binding factor alpha A subunit; Polyomavirus enhancer-binding protein 2 alpha Asubunit; Polyomavirus enhancer-binding protein 2 alpha A subunit; PEBP2-alpha A; PEBP2A; PEA2-alpha A; Osteoblast-specific transcription factor 2; OSF-2; OSF2; Oncogene AML-3; Core-binding factor subunit alpha-1; CBF-alpha-1; CBFA1; AML3; Acute myeloid leukemia 3 protein
Evidence Score (E-score) 3 Experimental Evidences +
1 Affinity Capture-Western [86]
2 Affinity Capture-Western [87]
3 Reconstituted Complex [86]
Interacting Target Name Hypoxia-inducible factor 2 alpha (HIF-2A) Successful Target Interacting Protein Info
UniProt ID
EPAS1_HUMAN 
Synonyms PASD2; PAS domain-containing protein 2; MOP2; Member of PAS protein 2; Hypoxia-inducible factor 2-alpha; HLF; HIF-2-alpha; HIF2-alpha; HIF2A; HIF-1-alpha-like factor; EPAS-1; Endothelial PAS domain-containing protein 1; Class E basic helix-loop-helix protein 73; bHLHe73; Basic-helix-loop-helix-PAS protein MOP2
Evidence Score (E-score) 2 Experimental Evidences +
1 Co-localization [88]
2 Yeast two hybrid [29]
Interacting Target Name Cyclic-AMP-dependent transcription factor ATF-3 (ATF3) Literature-reported Target Interacting Protein Info
UniProt ID
ATF3_HUMAN 
Synonyms Cyclic AMP-dependent transcription factor ATF-3; cAMP-dependent transcription factor ATF-3; Activating transcription factor 3
Evidence Score (E-score) 2 Experimental Evidences +
1 Affinity Capture-Western [89]
2 Reconstituted Complex [90]
Interacting Target Name Zinc finger E-box-binding homeobox 2 (ZEB2) Literature-reported Target Interacting Protein Info
UniProt ID
ZEB2_HUMAN 
Synonyms Zinc finger homeobox protein 1b; ZFX1B; ZFHX1B; SMADIP1; Smad-interacting protein 1; SIP1; KIAA0569; HRIHFB2411
Evidence Score (E-score) 2 Experimental Evidences +
1 Affinity Capture-Western [91]
2 Yeast two hybrid [29]
Interacting Protein Name Forkhead box protein O3 (FOXO3) Interacting Protein Interacting Protein Info
UniProt ID
FOXO3_HUMAN 
Synonyms AF6q21; FKHRL1; FKHRL1P2; FOXO2; FOXO3A
Evidence Score (E-score) 2 Experimental Evidences +
1 Affinity Capture-Western [53]
2 Co-localization [92]
Interacting Protein Name Transcriptional repressor protein YY1 (YY1) Interacting Protein Interacting Protein Info
UniProt ID
TYY1_HUMAN 
Synonyms DELTA; INO80S; NF.E1; UCRBP; YIN.YANG.1
Evidence Score (E-score) 2 Experimental Evidences +
1 Affinity Capture-MS [11]
2 Reconstituted Complex [93]
Interacting Protein Name Mad-related protein 1 (SMAD1) Interacting Protein Interacting Protein Info
UniProt ID
SMAD1_HUMAN 
Synonyms Transforming growth factor-beta-signaling protein 1; Smad1; SMAD family member 1; SMAD 1; Mothers against DPP homolog 1; Mothers against decapentaplegic homolog 1; Mad-related protein 1; MADR1; MADH1; MAD homolog 1; JV4-1; BSP-1; BSP1
Evidence Score (E-score) 2 Experimental Evidences +
1 Affinity Capture-MS [11]
2 Yeast two hybrid [76]
Ubiquitin related proteins [+] 1 Ubiquitin related proteins +
Interacting Target Name NEDD4-like E3 ubiquitin-protein ligase WWP2 (WWP2) Literature-reported Target Interacting Protein Info
UniProt ID
WWP2_HUMAN 
Synonyms WW domain-containing protein 2; WW domaincontaining protein 2; NEDD4like E3 ubiquitinprotein ligaseWWP2; HECT-type E3 ubiquitin transferase WWP2; Atrophin-1-interacting protein 2; Atrophin1interacting protein 2; AIP2
Evidence Score (E-score) 3 Experimental Evidences +
1 Affinity Capture-MS [11]
2 Affinity Capture-Western [94]
3 Yeast two hybrid [31]
Other proteins [+] 4 Other proteins +
Interacting Protein Name Zinc finger FYVE domain-containing protein 9 (ZFYVE9) Interacting Protein Interacting Protein Info
UniProt ID
ZFYV9_HUMAN 
Synonyms MADHIP; NSP; PPP1R173; SARA; SMADIP
Evidence Score (E-score) 7 Experimental Evidences +
1 Affinity Capture-MS [74]
2 Affinity Capture-MS [75]
3 Affinity Capture-MS [11]
4 Affinity Capture-Western [95]
5 Co-crystal Structure [69]
6 Reconstituted Complex [69]
7 Yeast two hybrid [29]
Interacting Protein Name Inner nuclear membrane protein Man1 (LEMD3) Interacting Protein Interacting Protein Info
UniProt ID
MAN1_HUMAN 
Synonyms MAN1
Evidence Score (E-score) 4 Experimental Evidences +
1 Affinity Capture-MS [74]
2 Affinity Capture-MS [75]
3 Affinity Capture-MS [11]
4 Reconstituted Complex [22]
Interacting Target Name Enhancer of filamentation 1 (NEDD9) Literature-reported Target Interacting Protein Info
UniProt ID
CASL_HUMAN 
Synonyms Renal carcinoma antigen NY-REN-12; p105; Neural precursor cell expressed developmentally down-regulated protein 9; NEDD-9; hEF1; CRK-associated substrate-related protein; CASS2; CAS-L; CasL; Cas scaffolding protein family member 2
Evidence Score (E-score) 3 Experimental Evidences +
1 Affinity Capture-Western [96]
2 Reconstituted Complex [97]
3 Yeast two hybrid [96]
Interacting Protein Name SNW domain-containing protein 1 (SNW1) Interacting Protein Interacting Protein Info
UniProt ID
SNW1_HUMAN 
Synonyms Bx42; NCOA.62; PRPF45; Prp45; SKIIP; SKIP
Evidence Score (E-score) 2 Experimental Evidences +
1 Affinity Capture-MS [11]
2 Yeast two hybrid [98]
References  Top
REF 1 Evidence for a role of MSK1 in transforming growth factor-beta-mediated responses through p38alpha and Smad signaling pathways. J Biol Chem. 2004 Jul 16;279(29):30474-9.
REF 2 Regulation of Smad signaling through a differential recruitment of coactivators and corepressors by ZEB proteins. EMBO J. 2003 May 15;22(10):2453-62.
REF 3 AMP-activated protein kinase inhibits TGF--induced fibrogenic responses of hepatic stellate cells by targeting transcriptional coactivator p300. J Cell Physiol. 2012 Mar;227(3):1081-9.
REF 4 Histone deacetylase inhibition suppresses the transforming growth factor beta1-induced epithelial-to-mesenchymal transition in hepatocytes. Hepatology. 2010 Sep;52(3):1033-45.
REF 5 TGF-beta-stimulated cooperation of smad proteins with the coactivators CBP/p300. Genes Dev. 1998 Jul 15;12(14):2114-9.
REF 6 Involvement of the constitutive complex formation of c-Ski/SnoN with Smads in the impaired negative feedback regulation of transforming growth fact... Arthritis Rheum. 2007 May;56(5):1694-705.
REF 7 The transcriptional co-activator P/CAF potentiates TGF-beta/Smad signaling. Nucleic Acids Res. 2000 Nov 1;28(21):4291-8.
REF 8 Sox9 and p300 cooperatively regulate chromatin-mediated transcription. J Biol Chem. 2005 Oct 21;280(42):35203-8.
REF 9 c-Ski acts as a transcriptional co-repressor in transforming growth factor-beta signaling through interaction with smads. J Biol Chem. 1999 Dec 3;274(49):35269-77.
REF 10 TRAF4 promotes TGF- receptor signaling and drives breast cancer metastasis. Mol Cell. 2013 Sep 12;51(5):559-72.
REF 11 Identification of novel Smad2 and Smad3 associated proteins in response to TGF-beta1. J Cell Biochem. 2008 Oct 1;105(2):596-611.
REF 12 Negative regulation of transforming growth factor-beta (TGF-beta) signaling by WW domain-containing protein 1 (WWP1). Oncogene. 2004 Sep 9;23(41):6914-23.
REF 13 Selective compounds enhance osteoblastic activity by targeting HECT domain of ubiquitin ligase Smurf1. Oncotarget. 2016 Jul 18;8(31):50521-50533.
REF 14 Regulation of Smad degradation and activity by Smurf2, an E3 ubiquitin ligase. Proc Natl Acad Sci U S A. 2001 Jan 30;98(3):974-9.
REF 15 Smurf2 is a ubiquitin E3 ligase mediating proteasome-dependent degradation of Smad2 in transforming growth factor-beta signaling. J Biol Chem. 2000 Nov 24;275(47):36818-22.
REF 16 Characterization of a novel transcriptionally active domain in the transforming growth factor beta-regulated Smad3 protein. Nucleic Acids Res. 2005 Jul 1;33(12):3708-21.
REF 17 Regulation of transforming growth factor-beta and bone morphogenetic protein signalling by transcriptional coactivator GCN5. Genes Cells. 2004 Feb;9(2):143-51.
REF 18 A novel function for p21Cip1 and acetyltransferase p/CAF as critical transcriptional regulators of TGF-mediated breast cancer cell migration and invasion. Breast Cancer Res. 2012 Sep 20;14(5):R127.
REF 19 NEDD4-2 (neural precursor cell expressed, developmentally down-regulated 4-2) negatively regulates TGF-beta (transforming growth factor-beta) signalling by inducing ubiquitin-mediated degradation of Smad2 and TGF-beta type I receptor. Biochem J. 2005 Mar 15;386(Pt 3):461-70.
REF 20 A Smad action turnover switch operated by WW domain readers of a phosphoserine code. Genes Dev. 2011 Jun 15;25(12):1275-88.
REF 21 Structural basis for the versatile interactions of Smad7 with regulator WW domains in TGF- Pathways. Structure. 2012 Oct 10;20(10):1726-36.
REF 22 Ubiquitin ligase Nedd4L targets activated Smad2/3 to limit TGF-beta signaling. Mol Cell. 2009 Nov 13;36(3):457-68.
REF 23 Smad7 Protein Interacts with Receptor-regulated Smads (R-Smads) to Inhibit Transforming Growth Factor- (TGF-)/Smad Signaling. J Biol Chem. 2016 Jan 1;291(1):382-92.
REF 24 TGFbeta1/Smad3 counteracts BRCA1-dependent repair of DNA damage. Oncogene. 2005 Mar 31;24(14):2289-97.
REF 25 BRCA1 interacts with Smad3 and regulates Smad3-mediated TGF-beta signaling during oxidative stress responses. PLoS One. 2009 Sep 21;4(9):e7091.
REF 26 Genome-wide impact of the BRG1 SWI/SNF chromatin remodeler on the transforming growth factor beta transcriptional program. J Biol Chem. 2008 Jan 11;283(2):1146-55.
REF 27 Sustained activation of SMAD3/SMAD4 by FOXM1 promotes TGF--dependent cancer metastasis. J Clin Invest. 2014 Feb;124(2):564-79.
REF 28 An integrated bioinformatics platform for investigating the human E3 ubiquitin ligase-substrate interaction network. Nat Commun. 2017 Aug 24;8(1):347.
REF 29 Functional proteomics mapping of a human signaling pathway. Genome Res. 2004 Jul;14(7):1324-32. Validation Studies
REF 30 A proteome-scale map of the human interactome network. Cell. 2014 Nov 20;159(5):1212-1226.
REF 31 Widespread macromolecular interaction perturbations in human genetic disorders. Cell. 2015 Apr 23;161(3):647-660.
REF 32 Erbin inhibits transforming growth factor beta signaling through a novel Smad-interacting domain. Mol Cell Biol. 2007 Sep;27(17):6183-94.
REF 33 Identification of three novel Smad binding proteins involved in cell polarity. FEBS Lett. 2003 Mar 27;539(1-3):167-73.
REF 34 An essential role for Mad homology domain 1 in the association of Smad3 with histone deacetylase activity*. J Biol Chem. 2001 Jun 22;276(25):22595-603.
REF 35 Pin1 down-regulates transforming growth factor-beta (TGF-beta) signaling by inducing degradation of Smad proteins. J Biol Chem. 2009 Mar 6;284(10):6109-15.
REF 36 Pin1 promotes transforming growth factor-beta-induced migration and invasion. J Biol Chem. 2010 Jan 15;285(3):1754-64.
REF 37 Cyclin-dependent kinases regulate the antiproliferative function of Smads. Nature. 2004 Jul 8;430(6996):226-31.
REF 38 Using an in situ proximity ligation assay to systematically profile endogenous protein-protein interactions in a pathway network. J Proteome Res. 2014 Dec 5;13(12):5339-46.
REF 39 Disruption of transforming growth factor-beta signaling in ELF beta-spectrin-deficient mice. Science. 2003 Jan 24;299(5606):574-7.
REF 40 Casein kinase 2 (CK2) phosphorylates the deubiquitylase OTUB1 at Ser16 to trigger its nuclear localization. Sci Signal. 2015 Apr 14;8(372):ra35.
REF 41 SMAD3 represses androgen receptor-mediated transcription. Cancer Res. 2001 Mar 1;61(5):2112-8.
REF 42 The androgen receptor represses transforming growth factor-beta signaling through interaction with Smad3. J Biol Chem. 2002 Jan 11;277(2):1240-8.
REF 43 Differential modulation of androgen receptor-mediated transactivation by Smad3 and tumor suppressor Smad4. J Biol Chem. 2002 Nov 15;277(46):43749-56.
REF 44 USP15 is a deubiquitylating enzyme for receptor-activated SMADs. Nat Cell Biol. 2011 Sep 25;13(11):1368-75.
REF 45 USP15 stabilizes TGF- receptor I and promotes oncogenesis through the activation of TGF- signaling in glioblastoma. Nat Med. 2012 Feb 19;18(3):429-35.
REF 46 Ubiquitin carboxyl-terminal hydrolase-L5 promotes TGF-1 signaling by de-ubiquitinating and stabilizing Smad2/Smad3 in pulmonary fibrosis. Sci Rep. 2016 Sep 8;6:33116.
REF 47 The deubiquitinating enzyme UCH37 interacts with Smads and regulates TGF-beta signalling. Oncogene. 2005 Dec 1;24(54):8080-4.
REF 48 Toward an understanding of the protein interaction network of the human liver. Mol Syst Biol. 2011 Oct 11;7:536.
REF 49 Links between tumor suppressors: p53 is required for TGF-beta gene responses by cooperating with Smads. Cell. 2003 May 2;113(3):301-14.
REF 50 Differential regulation of the REG-proteasome pathway by p53/TGF- signalling and mutant p53 in cancer cells. Nat Commun. 2013;4:2667.
REF 51 A component of the ARC/Mediator complex required for TGF beta/Nodal signalling. Nature. 2002 Aug 8;418(6898):641-6.
REF 52 Smad7 induces tumorigenicity by blocking TGF-beta-induced growth inhibition and apoptosis. Exp Cell Res. 2005 Jul 1;307(1):231-46.
REF 53 Integration of Smad and forkhead pathways in the control of neuroepithelial and glioblastoma cell proliferation. Cell. 2004 Apr 16;117(2):211-23.
REF 54 TGF-beta receptor-mediated signalling through Smad2, Smad3 and Smad4. EMBO J. 1997 Sep 1;16(17):5353-62.
REF 55 Hyaluronan activates Hyal-2/WWOX/Smad4 signaling and causes bubbling cell death when the signaling complex is overexpressed. Oncotarget. 2017 Mar 21;8(12):19137-19155.
REF 56 Cooperative binding of Smad proteins to two adjacent DNA elements in the plasminogen activator inhibitor-1 promoter mediates transforming growth factor beta-induced smad-dependent transcriptional activation. J Biol Chem. 1999 Apr 2;274(14):9431-41.
REF 57 Structural mechanism of Smad4 recognition by the nuclear oncoprotein Ski: insights on Ski-mediated repression of TGF-beta signaling. Cell. 2002 Nov 1;111(3):357-67.
REF 58 Roles of pathway-specific and inhibitory Smads in activin receptor signaling. Mol Endocrinol. 1999 Jan;13(1):15-23.
REF 59 Sp1 and Smad proteins cooperate to mediate transforming growth factor-beta 1-induced alpha 2(I) collagen expression in human glomerular mesangial cells. J Biol Chem. 2001 Mar 9;276(10):6983-92.
REF 60 Transforming growth factor-1 induces expression of human coagulation factor XII via Smad3 and JNK signaling pathways in human lung fibroblasts. J Biol Chem. 2010 Apr 9;285(15):11638-51.
REF 61 Distinct domain utilization by Smad3 and Smad4 for nucleoporin interaction and nuclear import. J Biol Chem. 2003 Oct 24;278(43):42569-77.
REF 62 CBL enhances breast tumor formation by inhibiting tumor suppressive activity of TGF- signaling. Oncogene. 2012 Dec 13;31(50):5123-31.
REF 63 Structural basis of heteromeric smad protein assembly in TGF-beta signaling. Mol Cell. 2004 Sep 10;15(5):813-23.
REF 64 Direct interaction of Ski with either Smad3 or Smad4 is necessary and sufficient for Ski-mediated repression of transforming growth factor-beta signaling. J Biol Chem. 2003 Aug 29;278(35):32489-92.
REF 65 Two short segments of Smad3 are important for specific interaction of Smad3 with c-Ski and SnoN. J Biol Chem. 2003 Jan 3;278(1):531-6.
REF 66 Requirement of the co-repressor homeodomain-interacting protein kinase 2 for ski-mediated inhibition of bone morphogenetic protein-induced transcri... J Biol Chem. 2003 Oct 3;278(40):38998-9005.
REF 67 Competition between Ski and CREB-binding protein for binding to Smad proteins in transforming growth factor-beta signaling. J Biol Chem. 2007 Apr 13;282(15):11365-76.
REF 68 The oncoprotein Ski acts as an antagonist of transforming growth factor-beta signaling by suppressing Smad2 phosphorylation. J Biol Chem. 2003 Jul 11;278(28):26249-57.
REF 69 Smad3 allostery links TGF-beta receptor kinase activation to transcriptional control. Genes Dev. 2002 Aug 1;16(15):1950-63.
REF 70 Repression of bone morphogenetic protein and activin-inducible transcription by Evi-1. J Biol Chem. 2005 Jun 24;280(25):24227-37.
REF 71 Phosphatidylinositol 3-kinase/Akt pathway targets acetylation of Smad3 through Smad3/CREB-binding protein interaction: contribution to transforming growth factor beta1-induced Epstein-Barr virus reactivation. J Biol Chem. 2009 Sep 4;284(36):23912-24.
REF 72 Role of RbBP5 and H3K4me3 in the vicinity of Snail transcription start site during epithelial-mesenchymal transition in prostate cancer cell. Oncotarget. 2016 Oct 4;7(40):65553-65567.
REF 73 Selective inhibition of TGF-beta responsive genes by Smad-interacting peptide aptamers from FoxH1, Lef1 and CBP. Oncogene. 2005 Jun 2;24(24):3864-74.
REF 74 Architecture of the human interactome defines protein communities and disease networks. Nature. 2017 May 25;545(7655):505-509.
REF 75 The BioPlex Network: A Systematic Exploration of the Human Interactome. Cell. 2015 Jul 16;162(2):425-440.
REF 76 Heteromeric and homomeric interactions correlate with signaling activity and functional cooperativity of Smad3 and Smad4/DPC4. Mol Cell Biol. 1997 May;17(5):2521-8.
REF 77 Smad3 and Smad4 cooperate with c-Jun/c-Fos to mediate TGF-beta-induced transcription. Nature. 1998 Aug 27;394(6696):909-13.
REF 78 Tumor necrosis factor-alpha inhibits transforming growth factor-beta /Smad signaling in human dermal fibroblasts via AP-1 activation. J Biol Chem. 2000 Sep 29;275(39):30226-31.
REF 79 Smads bind directly to the Jun family of AP-1 transcription factors. Proc Natl Acad Sci U S A. 1999 Apr 27;96(9):4844-9.
REF 80 A cross-talk between hypoxia and TGF-beta orchestrates erythropoietin gene regulation through SP1 and Smads. J Mol Biol. 2004 Feb 6;336(1):9-24.
REF 81 Synergistic cooperation between hypoxia and transforming growth factor-beta pathways on human vascular endothelial growth factor gene expression. J Biol Chem. 2001 Oct 19;276(42):38527-35.
REF 82 Smad3 recruits the anaphase-promoting complex for ubiquitination and degradation of SnoN. Genes Dev. 2001 Nov 1;15(21):2822-36.
REF 83 Negative feedback regulation of TGF-beta signaling by the SnoN oncoprotein. Science. 1999 Oct 22;286(5440):771-4.
REF 84 Smad-Sp1 complexes mediate TGFbeta-induced early transcription of oncogenic Smad7 in pancreatic cancer cells. Carcinogenesis. 2006 Dec;27(12):2392-401.
REF 85 Role of Smad proteins and transcription factor Sp1 in p21(Waf1/Cip1) regulation by transforming growth factor-beta. J Biol Chem. 2000 Sep 22;275(38):29244-56.
REF 86 A RUNX2/PEBP2alpha A/CBFA1 mutation displaying impaired transactivation and Smad interaction in cleidocranial dysplasia. Proc Natl Acad Sci U S A. 2000 Sep 12;97(19):10549-54.
REF 87 Interaction and functional cooperation of PEBP2/CBF with Smads. Synergistic induction of the immunoglobulin germline Calpha promoter. J Biol Chem. 1999 Oct 29;274(44):31577-82.
REF 88 Opposite functions of HIF- isoforms in VEGF induction by TGF-1 under non-hypoxic conditions. Oncogene. 2011 Mar 10;30(10):1213-28.
REF 89 ATF3, an adaptive-response gene, enhances TGF{beta} signaling and cancer-initiating cell features in breast cancer cells. J Cell Sci. 2010 Oct 15;123(Pt 20):3558-65.
REF 90 A self-enabling TGFbeta response coupled to stress signaling: Smad engages stress response factor ATF3 for Id1 repression in epithelial cells. Mol Cell. 2003 Apr;11(4):915-26.
REF 91 Pc2-mediated sumoylation of Smad-interacting protein 1 attenuates transcriptional repression of E-cadherin. J Biol Chem. 2005 Oct 21;280(42):35477-89.
REF 92 SMAD3 augments FoxO3-induced MuRF-1 promoter activity in a DNA-binding-dependent manner. Am J Physiol Cell Physiol. 2014 Aug 1;307(3):C278-87.
REF 93 Nuclear factor YY1 inhibits transforming growth factor beta- and bone morphogenetic protein-induced cell differentiation. Mol Cell Biol. 2003 Jul;23(13):4494-510.
REF 94 Selective targeting of activating and inhibitory Smads by distinct WWP2 ubiquitin ligase isoforms differentially modulates TGF signalling and EMT. Oncogene. 2011 May 26;30(21):2451-62.
REF 95 Cytoplasmic PML function in TGF-beta signalling. Nature. 2004 Sep 9;431(7005):205-11.
REF 96 A novel ability of Smad3 to regulate proteasomal degradation of a Cas family member HEF1. EMBO J. 2000 Dec 15;19(24):6759-69.
REF 97 Direct interaction between Smad3, APC10, CDH1 and HEF1 in proteasomal degradation of HEF1. BMC Cell Biol. 2004 May 16;5:20.
REF 98 Ski-interacting protein interacts with Smad proteins to augment transforming growth factor-beta-dependent transcription. J Biol Chem. 2001 May 25;276(21):18243-8.

If You Find Any Error in Data or Bug in Web Service, Please Kindly Report It to Dr. Zhou and Dr. Zhang.