Target Interacting Protein Information

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Target General Information
Target Interacting Proteins
References
Target General Information  Top
Target ID T06273 Target Info
Target Name Poly [ADP-ribose] polymerase 1 (PARP1)
Synonyms Protein poly-ADP-ribosyltransferase PARP1; Poly[ADP-ribose] synthetase-1; Poly[ADP-ribose] synthase 1; Poly(ADP-ribose)polymerase-1; PPOL; PARP-1; NAD(+)Poly [ADP-ribose] polymerase-1 ADP-ribosyltransferase-1; NAD(+) ADP-ribosyltransferase-1; NAD(+) ADP-ribosyltransferase 1; DNA ADP-ribosyltransferase PARP1; ARTD1; ADPRT 1; ADPRT; ADP-ribosyltransferase diphtheria toxin-like 1
Target Type Successful Target
Gene Name PARP1
Biochemical Class Glycosyltransferases
UniProt ID
PARP1_HUMAN 
Target Interacting Proteins  Top
Acid anhydride hydrolases [+] 2 Acid anhydride hydrolases +
Interacting Target Name X-ray repair cross-complementing 5 (Ku80) Literature-reported Target Interacting Protein Info
UniProt ID
XRCC5_HUMAN 
Synonyms X-ray repair cross-complementing protein 5; X-ray repair complementing defective repair in Chinese hamster cells 5 (double-strand-break rejoining); TLAA; Thyroid-lupus autoantigen; Nuclear factor IV; Lupus Ku autoantigen protein p86; Ku86; G22P2; DNA repair protein XRCC5; CTCBF; CTC85; CTC box-binding factor 85 kDa subunit; ATP-dependent DNA helicase II 80 kDa subunit; ATP-dependent DNA helicase 2 subunit 2; 86 kDa subunit of Ku antigen
Evidence Score (E-score) 8 Experimental Evidences +
1 Affinity Capture-MS [1]
2 Affinity Capture-Western [2]
3 Affinity Capture-Western [3]
4 Affinity Capture-Western [4]
5 Biochemical Activity [5]
6 Reconstituted Complex [4]
7 Reconstituted Complex [5]
8 Reconstituted Complex [6]
Interacting Protein Name X-ray repair cross-complementing protein 6 (XRCC6) Interacting Protein Interacting Protein Info
UniProt ID
XRCC6_HUMAN 
Synonyms CTC75; CTCBF; G22P1; KU70; ML8; TLAA
Evidence Score (E-score) 8 Experimental Evidences +
1 Affinity Capture-MS [1]
2 Affinity Capture-Western [2]
3 Affinity Capture-Western [3]
4 Affinity Capture-Western [4]
5 Biochemical Activity [5]
6 Reconstituted Complex [4]
7 Reconstituted Complex [5]
8 Reconstituted Complex [6]
Acyltransferases [+] 2 Acyltransferases +
Interacting Target Name Histone acetyltransferase p300 (EP300) Clinical trial Target Interacting Protein Info
UniProt ID
EP300_HUMAN 
Synonyms Protein propionyltransferase p300; p300 HAT; P300; Histone crotonyltransferase p300; Histone butyryltransferase p300; E1A-associated protein p300; E1Aassociated protein p300
Evidence Score (E-score) 5 Experimental Evidences +
1 Affinity Capture-Western [7]
2 Affinity Capture-Western [8]
3 Affinity Capture-Western [9]
4 Affinity Capture-Western [10]
5 Reconstituted Complex [8]
Interacting Protein Name E3 ubiquitin-protein ligase CHFR (CHFR) Interacting Protein Interacting Protein Info
UniProt ID
CHFR_HUMAN 
Synonyms RNF116; RNF196
Evidence Score (E-score) 3 Experimental Evidences +
1 Affinity Capture-Western [11]
2 Affinity Capture-Western [12]
3 Biochemical Activity [13]
Carbon-nitrogen hydrolases [+] 1 Carbon-nitrogen hydrolases +
Interacting Target Name Histone deacetylase 1 (HDAC1) Successful Target Interacting Protein Info
UniProt ID
HDAC1_HUMAN 
Synonyms RPD3L1; HD1
Evidence Score (E-score) 2 Experimental Evidences +
1 Affinity Capture-Western [14]
2 Biochemical Activity [15]
Histones [+] 1 Histones +
Interacting Target Name Gamma-Histone H2AX (H2AFX) Literature-reported Target Interacting Protein Info
UniProt ID
H2AX_HUMAN 
Synonyms Phosphorylation of H2AX; Histone H2AX; Histone H2A.x; H2AX; H2a/x
Evidence Score (E-score) 3 Experimental Evidences +
1 Affinity Capture-MS [16]
2 Affinity Capture-Western [16]
3 Reconstituted Complex [17]
Isomerases [+] 1 Isomerases +
Interacting Target Name DNA topoisomerase I (TOP1) Successful Target Interacting Protein Info
UniProt ID
TOP1_HUMAN 
Synonyms DNA topoisomerase I
Evidence Score (E-score) 5 Experimental Evidences +
1 Affinity Capture-MS [18]
2 Affinity Capture-Western [19]
3 Co-fractionation [19]
4 Fluorescence Resonance Energy Transfer [19]
5 Reconstituted Complex [19]
Kinases [+] 3 Kinases +
Interacting Protein Name DNA polymerase beta (POLB) Interacting Protein Interacting Protein Info
UniProt ID
DPOLB_HUMAN 
Evidence Score (E-score) 3 Experimental Evidences +
1 Affinity Capture-Western [20]
2 Affinity Capture-Western [21]
3 Reconstituted Complex [20]
Interacting Target Name Cyclin-dependent kinase 8 (CDK8) Clinical trial Target Interacting Protein Info
UniProt ID
CDK8_HUMAN 
Synonyms Protein kinase K35; Mediator of RNA polymerase II transcription subunit CDK8; Mediator complex subunit CDK8; Cell division protein kinase 8
Evidence Score (E-score) 2 Experimental Evidences +
1 Affinity Capture-Western [22]
2 Reconstituted Complex [8]
Interacting Target Name Epidermal growth factor receptor (EGFR) Successful Target Interacting Protein Info
UniProt ID
EGFR_HUMAN 
Synonyms Epidermal growth factor receptor variant III; Proto-oncogene c-ErbB-1 variant III; Receptor tyrosine-protein kinase erbB-1 variant III; EGFR variant III; ERBB variant III; ERBB1 variant III; HER1 variant III
Evidence Score (E-score) 1 Experimental Evidences +
1 Affinity Capture-MS [23]
Lyases [+] 1 Lyases +
Interacting Protein Name Aprataxin and PNK-like factor (APLF) Interacting Protein Interacting Protein Info
UniProt ID
APLF_HUMAN 
Synonyms APFL; C2orf13; PALF; Xip1; ZCCHH1
Evidence Score (E-score) 2 Experimental Evidences +
1 Biochemical Activity [13]
2 Reconstituted Complex [24]
Mediator complexes [+] 1 Mediator complexes +
Interacting Protein Name Mediator of RNA polymerase II transcription subunit 6 (MED6) Interacting Protein Interacting Protein Info
UniProt ID
MED6_HUMAN 
Synonyms ARC33; NY.REN.28
Evidence Score (E-score) 2 Experimental Evidences +
1 Affinity Capture-Western [8]
2 Reconstituted Complex [22]
Nuclear hormone receptors [+] 1 Nuclear hormone receptors +
Interacting Target Name Estrogen receptor (ESR) Successful Target Interacting Protein Info
UniProt ID
ESR1_HUMAN 
Synonyms Oestrogen receptor alpha; Oestrogen receptor; Nuclear receptor subfamily 3 group A member 1; NR3A1; Estrogen receptor alpha; Estradiol receptor; ESR; ER-alpha; ERalpha; ER
Evidence Score (E-score) 2 Experimental Evidences +
1 Affinity Capture-MS [25]
2 Affinity Capture-Western [2]
Pore-forming toxins [+] 1 Pore-forming toxins +
Interacting Target Name Cellular tumor antigen p53 (TP53) Clinical trial Target Interacting Protein Info
UniProt ID
P53_HUMAN 
Synonyms Tumor suppressor p53; Phosphoprotein p53; P53; Antigen NY-CO-13
Evidence Score (E-score) 3 Experimental Evidences +
1 Affinity Capture-Western [26]
2 Reconstituted Complex [27]
3 Reconstituted Complex [28]
Transcription regulators [+] 3 Transcription regulators +
Interacting Protein Name Transcription factor p65 (RELA) Interacting Protein Interacting Protein Info
UniProt ID
TF65_HUMAN 
Synonyms NFKB3; p65
Evidence Score (E-score) 3 Experimental Evidences +
1 Affinity Capture-Western [7]
2 Reconstituted Complex [29]
3 Reconstituted Complex [8]
Interacting Target Name DNA-binding factor KBF1 (p105) Clinical trial Target Interacting Protein Info
UniProt ID
NFKB1_HUMAN 
Synonyms Nuclear factor of kappa light polypeptide gene enhancer in B-cells 1; Nuclear factor of kappa light polypeptide gene enhancer in Bcells 1; Nuclear factor NFkappaB p50 subunit; Nuclear factor NF-kappa-B p105 subunit; Nuclear factor NFkappaB p105 subunit; EBP-1; EBP1; DNA-binding factor KBF1; DNAbinding factor KBF1
Evidence Score (E-score) 2 Experimental Evidences +
1 Affinity Capture-Western [30]
2 Reconstituted Complex [8]
Interacting Target Name Transcription factor 7-like 2 (TCF7L2) Literature-reported Target Interacting Protein Info
UniProt ID
TF7L2_HUMAN 
Synonyms TCF-4; TCF4; T-cell-specific transcription factor 4; T-cell transcription factor-4; T-cell factor 4; HTCF-4; HMG box transcription factor 4
Evidence Score (E-score) 2 Experimental Evidences +
1 Affinity Capture-MS [31]
2 Affinity Capture-Western [3]
Other proteins [+] 3 Other proteins +
Interacting Target Name Proliferating cell nuclear antigen (PCNA) Clinical trial Target Interacting Protein Info
UniProt ID
PCNA_HUMAN 
Synonyms Cyclin
Evidence Score (E-score) 4 Experimental Evidences +
1 Affinity Capture-Western [20]
2 Affinity Capture-Western [32]
3 Affinity Capture-Western [21]
4 Reconstituted Complex [20]
Interacting Target Name Melanoma differentiation-associated protein 6 (CDKN1A) Literature-reported Target Interacting Protein Info
UniProt ID
CDN1A_HUMAN 
Synonyms WAF1; SDI1; PIC1; P21(WAF1); P21; Melanoma differentiation associated protein 6; MDA-6; MDA6; Cyclin-dependent kinase inhibitor 1; CIP1; CDK-interacting protein 1; CAP20
Evidence Score (E-score) 3 Experimental Evidences +
1 Affinity Capture-Western [20]
2 Affinity Capture-Western [21]
3 Reconstituted Complex [20]
Interacting Target Name Beta-catenin (CTNNB1) Successful Target Interacting Protein Info
UniProt ID
CTNB1_HUMAN 
Synonyms Wnt/beta-catenin signaling pathway; PRO2286; OK/SW-cl.35; CTNNB; Catenin beta-1
Evidence Score (E-score) 1 Experimental Evidences +
1 Affinity Capture-MS [33]
References  Top
REF 1 Poly(ADP-ribose) polymerase-1 modulates Nrf2-dependent transcription. Free Radic Biol Med. 2014 Feb;67:69-80.
REF 2 A topoisomerase IIbeta-mediated dsDNA break required for regulated transcription. Science. 2006 Jun 23;312(5781):1798-802.
REF 3 Ku70 and poly(ADP-ribose) polymerase-1 competitively regulate beta-catenin and T-cell factor-4-mediated gene transactivation: possible linkage of DNA damage recognition and Wnt signaling. Cancer Res. 2007 Feb 1;67(3):911-8.
REF 4 Poly(ADP-ribose) polymerase and Ku autoantigen form a complex and synergistically bind to matrix attachment sequences. J Biol Chem. 1999 Jul 16;274(29):20521-8.
REF 5 Identification and biochemical characterization of a Werner's syndrome protein complex with Ku70/80 and poly(ADP-ribose) polymerase-1. J Biol Chem. 2004 Apr 2;279(14):13659-67.
REF 6 In prostate cancer cells the interaction of C/EBPalpha with Ku70, Ku80, and poly(ADP-ribose) polymerase-1 increases sensitivity to DNA damage. J Biol Chem. 2006 Apr 28;281(17):11496-505.
REF 7 Protein arginine methyltransferase 1 coactivates NF-kappaB-dependent gene expression synergistically with CARM1 and PARP1. J Mol Biol. 2008 Mar 28;377(3):668-78.
REF 8 Acetylation of poly(ADP-ribose) polymerase-1 by p300/CREB-binding protein regulates coactivation of NF-kappaB-dependent transcription. J Biol Chem. 2005 Dec 9;280(49):40450-64.
REF 9 p300 cooperates with c-Jun and PARP-1 at the p300 binding site to activate RhoB transcription in NSC126188-mediated apoptosis. Biochim Biophys Acta. 2014 May;1839(5):364-73.
REF 10 Transcriptional coactivation of nuclear factor-kappaB-dependent gene expression by p300 is regulated by poly(ADP)-ribose polymerase-1. J Biol Chem. 2003 Nov 14;278(46):45145-53.
REF 11 CHFR protein regulates mitotic checkpoint by targeting PARP-1 protein for ubiquitination and degradation. J Biol Chem. 2012 Apr 13;287(16):12975-84.
REF 12 Small molecule inhibition of the CHFR-PARP1 interaction as novel approach to overcome intrinsic taxane resistance in cancer. Oncotarget. 2015 Oct 13;6(31):30773-86.
REF 13 Poly(ADP-ribose)-binding zinc finger motifs in DNA repair/checkpoint proteins. Nature. 2008 Jan 3;451(7174):81-5.
REF 14 Chromatin regulation by Brg1 underlies heart muscle development and disease. Nature. 2010 Jul 1;466(7302):62-7.
REF 15 SIRT1 promotes cell survival under stress by deacetylation-dependent deactivation of poly(ADP-ribose) polymerase 1. Mol Cell Biol. 2009 Aug;29(15):4116-29.
REF 16 The dynamic alterations of H2AX complex during DNA repair detected by a proteomic approach reveal the critical roles of Ca(2+)/calmodulin in the ionizing radiation-induced cell cycle arrest. Mol Cell Proteomics. 2006 Jun;5(6):1033-44.
REF 17 FACT-mediated exchange of histone variant H2AX regulated by phosphorylation of H2AX and ADP-ribosylation of Spt16. Mol Cell. 2008 Apr 11;30(1):86-97.
REF 18 Proteomic analysis of complexes formed by human topoisomerase I. Biochim Biophys Acta. 2005 May 20;1749(1):133-41.
REF 19 Poly(ADP-ribosyl)ation as a DNA damage-induced post-translational modification regulating poly(ADP-ribose) polymerase-1-topoisomerase I interaction. J Biol Chem. 2004 Sep 17;279(38):39686-96.
REF 20 p21CDKN1A participates in base excision repair by regulating the activity of poly(ADP-ribose) polymerase-1. DNA Repair (Amst). 2010 Jun 4;9(6):627-35.
REF 21 Epidermal growth factor receptor regulates beta-catenin location, stability, and transcriptional activity in oral cancer. Mol Cancer. 2010 Mar 19;9:64.
REF 22 PARP-1 determines specificity in a retinoid signaling pathway via direct modulation of mediator. Mol Cell. 2005 Apr 1;18(1):83-96.
REF 23 Importance of EGFR/ERCC1 interaction following radiation-induced DNA damage. Clin Cancer Res. 2014 Jul 1;20(13):3496-506.
REF 24 A novel human AP endonuclease with conserved zinc-finger-like motifs involved in DNA strand break responses. EMBO J. 2007 Apr 18;26(8):2094-103.
REF 25 Identification of proteins associated with ligand-activated estrogen receptor in human breast cancer cell nuclei by tandem affinity purification and nano LC-MS/MS. Proteomics. 2011 Jan;11(1):172-9.
REF 26 Aprataxin, a novel protein that protects against genotoxic stress. Hum Mol Genet. 2004 May 15;13(10):1081-93.
REF 27 Phosphorylation regulates the interaction and complex formation between wt p53 protein and PARP-1. J Cell Biochem. 2003 Aug 15;89(6):1260-84.
REF 28 PARP-1 binds E2F-1 independently of its DNA binding and catalytic domains, and acts as a novel coactivator of E2F-1-mediated transcription during re-entry of quiescent cells into S phase. Oncogene. 2003 Nov 20;22(52):8460-71.
REF 29 The enzymatic and DNA binding activity of PARP-1 are not required for NF-kappa B coactivator function. J Biol Chem. 2001 Dec 7;276(49):45588-97.
REF 30 Differential regulation of CXC ligand 1 transcription in melanoma cell lines by poly(ADP-ribose) polymerase-1. Oncogene. 2006 Dec 14;25(59):7714-22.
REF 31 Genetic and Proteomic Interrogation of Lower Confidence Candidate Genes Reveals Signaling Networks in -Catenin-Active Cancers. Cell Syst. 2016 Sep 28;3(3):302-316.e4.
REF 32 Human proliferating cell nuclear antigen, poly(ADP-ribose) polymerase-1, and p21waf1/cip1. A dynamic exchange of partners. J Biol Chem. 2003 Oct 10;278(41):39265-8.
REF 33 Beta-catenin interacts with the FUS proto-oncogene product and regulates pre-mRNA splicing. Gastroenterology. 2005 Oct;129(4):1225-36.

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