Target Interacting Protein Information

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Target General Information
Target Interacting Proteins
References
Target General Information  Top
Target ID T40097 Target Info
Target Name Stress-activated protein kinase JNK1 (JNK1)
Synonyms Stress-activated protein kinase 1c; SAPK1c; PRKM8; Mitogen-activated protein kinase 8; MAPK 8; MAP kinase 8; JNK-46; C-Jun N-terminal kinase 1
Target Type Clinical trial Target
Gene Name MAPK8
Biochemical Class Kinase
UniProt ID
MK08_HUMAN 
Target Interacting Proteins  Top
Acyltransferases [+] 1 Acyltransferases +
Interacting Protein Name Cyclic AMP-dependent transcription factor ATF-2 (ATF2) Interacting Protein Interacting Protein Info
UniProt ID
ATF2_HUMAN 
Synonyms CRE.BP1; CREB.2; CREB2; HB16; TREB7
Evidence Score (E-score) 13 Experimental Evidences +
1 Affinity Capture-Western [1]
2 Affinity Capture-Western [2]
3 Biochemical Activity [3]
4 Biochemical Activity [4]
5 Biochemical Activity [5]
6 Biochemical Activity [6]
7 Biochemical Activity [7]
8 Biochemical Activity [8]
9 Biochemical Activity [2]
10 Biochemical Activity [9]
11 Biochemical Activity [10]
12 Biochemical Activity [11]
13 Yeast two hybrid [12]
Alkyl/aryl transferases [+] 1 Alkyl/aryl transferases +
Interacting Target Name Glutathione S-transferase P (GSTP1) Clinical trial Target Interacting Protein Info
UniProt ID
GSTP1_HUMAN 
Synonyms GSTP1-1; GSTP11; GST3; GST class-pi; GST classpi; FAEES3
Evidence Score (E-score) 6 Experimental Evidences +
1 Affinity Capture-Western [13]
2 Affinity Capture-Western [14]
3 Affinity Capture-Western [15]
4 Co-localization [14]
5 Reconstituted Complex [13]
6 Reconstituted Complex [14]
Alpha-type channels [+] 1 Alpha-type channels +
Interacting Target Name Apoptosis regulator Bcl-xL (BCL-xL) Clinical trial Target Interacting Protein Info
UniProt ID
B2CL1_HUMAN 
Synonyms Bcl-XL; BCLX; Bcl-2-like protein 1; Bcl2like protein 1; Bcl2-L-1; Bcl2L1; BCL2L; Apoptosis regulator Bcl-X
Evidence Score (E-score) 2 Experimental Evidences +
1 Biochemical Activity [16]
2 Co-localization [17]
Ester hydrolases [+] 1 Ester hydrolases +
Interacting Protein Name Dual specificity protein phosphatase 8 (DUSP8) Interacting Protein Interacting Protein Info
UniProt ID
DUS8_HUMAN 
Synonyms VH5; Dual specificity protein phosphatase hVH-5; Dual specificity protein phosphatase 8; C11orf81
Evidence Score (E-score) 3 Experimental Evidences +
1 Affinity Capture-MS [18]
2 Affinity Capture-MS [19]
3 Yeast two hybrid [20]
Histones [+] 1 Histones +
Interacting Target Name Gamma-Histone H2AX (H2AFX) Literature-reported Target Interacting Protein Info
UniProt ID
H2AX_HUMAN 
Synonyms Phosphorylation of H2AX; Histone H2AX; Histone H2A.x; H2AX; H2a/x
Evidence Score (E-score) 2 Experimental Evidences +
1 Affinity Capture-Western [21]
2 Reconstituted Complex [22]
Kinases [+] 4 Kinases +
Interacting Target Name MAPK/ERK kinase kinase 1 (MAP3K1) Clinical trial Target Interacting Protein Info
UniProt ID
M3K1_HUMAN 
Synonyms Mitogen-activated protein kinase kinase kinase 1; Mitogenactivated protein kinase kinase kinase 1; MEKK1; MEKK 1; MEKK; MEK kinase 1; MAPKKK1
Evidence Score (E-score) 4 Experimental Evidences +
1 Affinity Capture-Western [23]
2 Affinity Capture-Western [24]
3 Biochemical Activity [23]
4 Co-localization [17]
Interacting Target Name Epidermal growth factor receptor (EGFR) Successful Target Interacting Protein Info
UniProt ID
EGFR_HUMAN 
Synonyms Epidermal growth factor receptor variant III; Proto-oncogene c-ErbB-1 variant III; Receptor tyrosine-protein kinase erbB-1 variant III; EGFR variant III; ERBB variant III; ERBB1 variant III; HER1 variant III
Evidence Score (E-score) 3 Experimental Evidences +
1 Affinity Capture-Luminescence [25]
2 Protein-Fragment Complementation Assay [25]
3 Yeast two hybrid [26]
Interacting Target Name Ribosomal protein S6 kinase beta-1 (S6K1) Clinical trial Target Interacting Protein Info
UniProt ID
KS6B1_HUMAN 
Synonyms STK14A; Serine/threonine-protein kinase 14A; S6K-beta-1; S6K; Ribosomal protein S6 kinase I; P70S6K1; p70-S6K 1; P70-S6K; p70 S6K-alpha; p70 S6KA; p70 S6 kinase alpha; p70 ribosomal S6 kinase alpha; 70 kDa ribosomal protein S6 kinase 1
Evidence Score (E-score) 3 Experimental Evidences +
1 Affinity Capture-Western [27]
2 Biochemical Activity [27]
3 Yeast two hybrid [28]
Interacting Target Name ERK activator kinase 7 (MAP2K7) Literature-reported Target Interacting Protein Info
UniProt ID
MP2K7_HUMAN 
Synonyms Stress-activated protein kinase kinase 4; SKK4; SAPKK-4; SAPKK4; SAPK kinase 4; PRKMK7; MKK7; Mitogen-activated protein kinase kinase 7; MEK7; MEK 7; MAPKK 7; MAPK/ERK kinase7; MAPK/ERK kinase 7; MAP kinase kinase 7; JNKK2; JNKK 2; JNK-activating kinase 2; JNK kinase 2; JNK activating kinase 2; Dual specificity mitogen-activated protein kinase kinase 7; C-Jun N-terminal kinase kinase 2
Evidence Score (E-score) 3 Experimental Evidences +
1 Biochemical Activity [3]
2 Co-localization [17]
3 Protein-peptide [29]
Pore-forming toxins [+] 2 Pore-forming toxins +
Interacting Target Name Cellular tumor antigen p53 (TP53) Clinical trial Target Interacting Protein Info
UniProt ID
P53_HUMAN 
Synonyms Tumor suppressor p53; Phosphoprotein p53; P53; Antigen NY-CO-13
Evidence Score (E-score) 8 Experimental Evidences +
1 Affinity Capture-Western [30]
2 Affinity Capture-Western [31]
3 Affinity Capture-Western [32]
4 Biochemical Activity [33]
5 Biochemical Activity [34]
6 Reconstituted Complex [35]
7 Reconstituted Complex [36]
8 Yeast two hybrid [37]
Interacting Target Name Amyloid beta A4 protein (APP) Successful Target Interacting Protein Info
UniProt ID
A4_HUMAN 
Synonyms Vascular beta-amyloid; Protease nexin-II; PreA4; PN-II; CVAP; Cerebral vascular amyloidpeptide; Cerebral vascular amyloid peptide; Beta-amyloid peptide; APPI; Amyloid-beta precursor protein; Amyloid-beta A4 protein; Amyloid precursor protein; Amyloid beta precursor protein; Alzheimer's disease amyloid protein; Alzheimer disease amyloid protein; AD1; ABPP; A4
Evidence Score (E-score) 2 Experimental Evidences +
1 Biochemical Activity [38]
2 Reconstituted Complex [39]
Transcription regulators [+] 2 Transcription regulators +
Interacting Target Name Transcription factor AP-1 (JUN) Discontinued Target Interacting Protein Info
UniProt ID
JUN_HUMAN 
Synonyms V-jun avian sarcoma virus 17 oncogene homolog; Transcription factor AP-1; Proto-oncogene c-jun; P39; C-jun proto-oncogene; AP-1 transcription factor; AP1; Activator protein-1; Activator protein 1
Evidence Score (E-score) 20 Experimental Evidences +
1 Affinity Capture-Western [40]
2 Affinity Capture-Western [41]
3 Biochemical Activity [42]
4 Biochemical Activity [43]
5 Biochemical Activity [44]
6 Biochemical Activity [45]
7 Biochemical Activity [40]
8 Biochemical Activity [46]
9 Biochemical Activity [47]
10 Biochemical Activity [48]
11 Biochemical Activity [49]
12 Biochemical Activity [50]
13 Biochemical Activity [51]
14 Biochemical Activity [27]
15 Biochemical Activity [52]
16 Biochemical Activity [53]
17 Biochemical Activity [54]
18 Biochemical Activity [55]
19 Biochemical Activity [56]
20 Biochemical Activity [57]
Interacting Protein Name ETS domain-containing protein Elk-1 (ELK1) Interacting Protein Interacting Protein Info
UniProt ID
ELK1_HUMAN 
Evidence Score (E-score) 4 Experimental Evidences +
1 Biochemical Activity [7]
2 Biochemical Activity [54]
3 Biochemical Activity [58]
4 Yeast two hybrid [12]
Other proteins [+] 6 Other proteins +
Interacting Protein Name WD repeat-containing protein 62 (WDR62) Interacting Protein Interacting Protein Info
UniProt ID
WDR62_HUMAN 
Synonyms C19orf14; MCPH2
Evidence Score (E-score) 5 Experimental Evidences +
1 Affinity Capture-MS [18]
2 Affinity Capture-MS [19]
3 Affinity Capture-Western [59]
4 Biochemical Activity [59]
5 Yeast two hybrid [59]
Interacting Target Name JNK-interacting protein 1 peptide (pepJIP1) Patented-recorded Target Interacting Protein Info
UniProt ID
JIP1_HUMAN 
Synonyms PRKM8IP; Mitogen-activated protein kinase 8-interacting protein 1; JNK-interacting protein 1; JNK MAP kinase scaffold protein 1; JIP-1; JIP1; Islet-brain 1; IB-1; IB1; C-Jun-amino-terminal kinase-interacting protein 1
Evidence Score (E-score) 3 Experimental Evidences +
1 Affinity Capture-Western [60]
2 Affinity Capture-Western [61]
3 Protein-peptide [29]
Interacting Target Name Insulin receptor substrate-1 (IRS1) Clinical trial Target Interacting Protein Info
UniProt ID
IRS1_HUMAN 
Synonyms IRS-1; Insulin receptor substrate 1
Evidence Score (E-score) 3 Experimental Evidences +
1 Affinity Capture-Western [62]
2 Reconstituted Complex [62]
3 Yeast two hybrid [63]
Interacting Target Name Apoptosis mediating surface antigen FAS (FAS) Clinical trial Target Interacting Protein Info
UniProt ID
TNR6_HUMAN 
Synonyms Tumor necrosis factor receptor superfamily member 6; TNFRSF6; FASLG receptor; FAS1; CD95; APT1; Apoptosis-mediating surface antigen FAS; Apo-1 antigen
Evidence Score (E-score) 2 Experimental Evidences +
1 Co-localization [17]
2 Co-purification [64]
Interacting Target Name Apoptosis antigen ligand (CD178) Literature-reported Target Interacting Protein Info
UniProt ID
TNFL6_HUMAN 
Synonyms Tumor necrosis factor ligand superfamily member 6; TNFSF6; FasL; Fas ligand; FAS antigen ligand; CD95-L; CD95L; CD95 ligand; CD178 antigen; CD178; APTL; APT1LG1; Apoptosis antigen ligand
Evidence Score (E-score) 2 Experimental Evidences +
1 Co-localization [17]
2 Co-purification [64]
Interacting Target Name Melanoma differentiation-associated protein 6 (CDKN1A) Literature-reported Target Interacting Protein Info
UniProt ID
CDN1A_HUMAN 
Synonyms WAF1; SDI1; PIC1; P21(WAF1); P21; Melanoma differentiation associated protein 6; MDA-6; MDA6; Cyclin-dependent kinase inhibitor 1; CIP1; CDK-interacting protein 1; CAP20
Evidence Score (E-score) 2 Experimental Evidences +
1 Affinity Capture-Western [65]
2 Biochemical Activity [66]
References  Top
REF 1 c-Jun NH2-terminal kinases target the ubiquitination of their associated transcription factors. J Biol Chem. 1997 Dec 19;272(51):32163-8.
REF 2 Jun N-terminal kinase activity and early growth-response factor-1 gene expression are down-regulated in Fanconi anemia group A lymphoblasts. Blood. 2004 Jan 1;103(1):128-32.
REF 3 Mitogen-activated protein kinase kinase 7 is an activator of the c-Jun NH2-terminal kinase. Proc Natl Acad Sci U S A. 1997 Jul 8;94(14):7337-42.
REF 4 Acetylation of a conserved lysine residue in the ATP binding pocket of p38 augments its kinase activity during hypertrophy of cardiomyocytes. Mol Cell Biol. 2011 Jun;31(11):2349-63.
REF 5 Regulation of stress-responsive mitogen-activated protein (MAP) kinase pathways by TAO2. J Biol Chem. 2001 May 11;276(19):16070-5.
REF 6 Characterization of the mitogen-activated protein kinase kinase 4 (MKK4)/c-Jun NH2-terminal kinase 1 and MKK3/p38 pathways regulated by MEK kinases 2 and 3. MEK kinase 3 activates MKK3 but does not cause activation of p38 kinase in vivo. J Biol Chem. 1997 May 30;272(22):14489-96.
REF 7 Selective interaction of JNK protein kinase isoforms with transcription factors. EMBO J. 1996 Jun 3;15(11):2760-70.
REF 8 IAP suppression of apoptosis involves distinct mechanisms: the TAK1/JNK1 signaling cascade and caspase inhibition. Mol Cell Biol. 2002 Mar;22(6):1754-66.
REF 9 Pellino3, a novel member of the Pellino protein family, promotes activation of c-Jun and Elk-1 and may act as a scaffolding protein. J Immunol. 2003 Aug 1;171(3):1500-6.
REF 10 Pellino2 activates the mitogen activated protein kinase pathway. FEBS Lett. 2003 Jun 19;545(2-3):199-202.
REF 11 Cbl-b, a member of the Sli-1/c-Cbl protein family, inhibits Vav-mediated c-Jun N-terminal kinase activation. Oncogene. 1997 Nov 20;15(21):2511-20.
REF 12 Nuclear factor of activated T3 is a negative regulator of Ras-JNK1/2-AP-1 induced cell transformation. Cancer Res. 2007 Sep 15;67(18):8725-35.
REF 13 Glutathione S-transferase P1-1 (GSTP1-1) inhibits c-Jun N-terminal kinase (JNK1) signaling through interaction with the C terminus. J Biol Chem. 2001 Jun 15;276(24):20999-1003.
REF 14 Phosphorylation of Glutathione S-Transferase P1 (GSTP1) by Epidermal Growth Factor Receptor (EGFR) Promotes Formation of the GSTP1-c-Jun N-terminal kinase (JNK) Complex and Suppresses JNK Downstream Signaling and Apoptosis in Brain Tumor Cells. J Biol Chem. 2015 Dec 25;290(52):30866-78.
REF 15 Human glutathione S-transferase P1-1 interacts with TRAF2 and regulates TRAF2-ASK1 signals. Oncogene. 2006 Sep 21;25(42):5787-800.
REF 16 Phospho-Bcl-x(L)(Ser62) plays a key role at DNA damage-induced G(2) checkpoint. Cell Cycle. 2012 Jun 1;11(11):2159-69.
REF 17 Using an in situ proximity ligation assay to systematically profile endogenous protein-protein interactions in a pathway network. J Proteome Res. 2014 Dec 5;13(12):5339-46.
REF 18 Architecture of the human interactome defines protein communities and disease networks. Nature. 2017 May 25;545(7655):505-509.
REF 19 The BioPlex Network: A Systematic Exploration of the Human Interactome. Cell. 2015 Jul 16;162(2):425-440.
REF 20 A directed protein interaction network for investigating intracellular signal transduction. Sci Signal. 2011 Sep 6;4(189):rs8.
REF 21 DNA damage during mitosis invokes a JNK-mediated stress response that leads to cell death. J Cell Biochem. 2010 Jun 1;110(3):725-31.
REF 22 Lymphokine-activated killer T-cell-originated protein kinase phosphorylation of histone H2AX prevents arsenite-induced apoptosis in RPMI7951 melanoma cells. Clin Cancer Res. 2006 Dec 1;12(23):6884-93.
REF 23 MEKK1 binds directly to the c-Jun N-terminal kinases/stress-activated protein kinases. J Biol Chem. 1997 Dec 19;272(51):32056-60.
REF 24 Negative regulation of MEKK1-induced signaling by glutathione S-transferase Mu. J Biol Chem. 2004 Oct 15;279(42):43589-94.
REF 25 The mammalian-membrane two-hybrid assay (MaMTH) for probing membrane-protein interactions in human cells. Nat Methods. 2014 May;11(5):585-92.
REF 26 In silico prediction of physical protein interactions and characterization of interactome orphans. Nat Methods. 2015 Jan;12(1):79-84.
REF 27 Phosphorylation and degradation of S6K1 (p70S6K1) in response to persistent JNK1 Activation. Biochim Biophys Acta. 2013 Dec;1832(12):1980-8.
REF 28 Toward an understanding of the protein interaction network of the human liver. Mol Syst Biol. 2011 Oct 11;7:536.
REF 29 Interacting JNK-docking sites in MKK7 promote binding and activation of JNK mitogen-activated protein kinases. J Biol Chem. 2006 May 12;281(19):13169-79.
REF 30 JNK1, JNK2 and JNK3 are p53 N-terminal serine 34 kinases. Oncogene. 1997 Nov 6;15(19):2277-87.
REF 31 Downregulation of p53 by sustained JNK activation during apoptosis. Mol Carcinog. 2000 Nov;29(3):179-88.
REF 32 Low doses of paclitaxel potently induce apoptosis in human retinoblastoma Y79 cells by up-regulating E2F1. Int J Oncol. 2008 Oct;33(4):677-87.
REF 33 Control of p53 multimerization by Ubc13 is JNK-regulated. Proc Natl Acad Sci U S A. 2009 Aug 4;106(31):12676-81.
REF 34 MEKK1/JNK signaling stabilizes and activates p53. Proc Natl Acad Sci U S A. 1998 Sep 1;95(18):10541-6.
REF 35 The effect of C-terminal fragment of JNK2 on the stability of p53 and cell proliferation. Cell Res. 2004 Oct;14(5):434-8.
REF 36 JNK targets p53 ubiquitination and degradation in nonstressed cells. Genes Dev. 1998 Sep 1;12(17):2658-63.
REF 37 JNK1 physically interacts with WW domain-containing oxidoreductase (WOX1) and inhibits WOX1-mediated apoptosis. J Biol Chem. 2003 Mar 14;278(11):9195-202.
REF 38 Amyloid beta protein precursor is phosphorylated by JNK-1 independent of, yet facilitated by, JNK-interacting protein (JIP)-1. J Biol Chem. 2003 Oct 24;278(43):42058-63.
REF 39 Interactions of pathological hallmark proteins: tubulin polymerization promoting protein/p25, beta-amyloid, and alpha-synuclein. J Biol Chem. 2011 Sep 30;286(39):34088-100.
REF 40 p57KIP2 modulates stress-activated signaling by inhibiting c-Jun NH2-terminal kinase/stress-activated protein Kinase. J Biol Chem. 2003 Nov 28;278(48):48092-8.
REF 41 Negative regulation of constitutive NF-kappaB and JNK signaling by PKN1-mediated phosphorylation of TRAF1. Genes Cells. 2008 May;13(5):509-20.
REF 42 TNF-RII and c-IAP1 mediate ubiquitination and degradation of TRAF2. Nature. 2002 Mar 21;416(6878):345-7.
REF 43 Regulation of Nur77 nuclear export by c-Jun N-terminal kinase and Akt. Oncogene. 2006 May 18;25(21):2974-86.
REF 44 The proto-oncogene c-Cbl is a positive regulator of Met-induced MAP kinase activation: a role for the adaptor protein Crk. Oncogene. 2000 Aug 17;19(35):4058-65.
REF 45 The TNF receptor family member CD27 signals to Jun N-terminal kinase via Traf-2. Eur J Immunol. 1998 Jul;28(7):2208-16.
REF 46 Critical roles of threonine 187 phosphorylation in cellular stress-induced rapid and transient activation of transforming growth factor-beta-activated kinase 1 (TAK1) in a signaling complex containing TAK1-binding protein TAB1 and TAB2. J Biol Chem. 2005 Feb 25;280(8):7359-68.
REF 47 Inhibiting TRAF2-mediated activation of NF-kappaB facilitates induction of AP-1. J Biol Chem. 2010 Apr 9;285(15):11617-27.
REF 48 3-Hydroxyanthranilic acid inhibits PDK1 activation and suppresses experimental asthma by inducing T cell apoptosis. Proc Natl Acad Sci U S A. 2007 Nov 20;104(47):18619-24.
REF 49 Fibroblast growth factor receptor-1-mediated endothelial cell proliferation is dependent on the Src homology (SH) 2/SH3 domain-containing adaptor protein Crk. J Biol Chem. 1999 Sep 3;274(36):25726-34.
REF 50 Contribution of the membrane-distal tyrosine in intracellular signaling by the granulocyte colony-stimulating factor receptor. J Biol Chem. 2004 Jan 2;279(1):326-40.
REF 51 TEL-JAK2 constitutively activates the extracellular signal-regulated kinase (ERK), stress-activated protein/Jun kinase (SAPK/JNK), and p38 signaling pathways. Blood. 2002 Aug 15;100(4):1438-48.
REF 52 The germinal center kinase TNIK is required for canonical NF-B and JNK signaling in B-cells by the EBV oncoprotein LMP1 and the CD40 receptor. PLoS Biol. 2012;10(8):e1001376.
REF 53 JNK1: a protein kinase stimulated by UV light and Ha-Ras that binds and phosphorylates the c-Jun activation domain. Cell. 1994 Mar 25;76(6):1025-37.
REF 54 Neither ERK nor JNK/SAPK MAP kinase subtypes are essential for histone H3/HMG-14 phosphorylation or c-fos and c-jun induction. J Cell Sci. 1995 Nov;108 ( Pt 11):3599-609.
REF 55 JNK2 contains a specificity-determining region responsible for efficient c-Jun binding and phosphorylation. Genes Dev. 1994 Dec 15;8(24):2996-3007.
REF 56 The MYND domain-containing protein BRAM1 inhibits lymphotoxin beta receptor-mediated signaling through affecting receptor oligomerization. Cell Signal. 2011 Jan;23(1):80-8.
REF 57 JNKK1 organizes a MAP kinase module through specific and sequential interactions with upstream and downstream components mediated by its amino-terminal extension. Genes Dev. 1998 Nov 1;12(21):3369-81.
REF 58 Induction of c-fos expression through JNK-mediated TCF/Elk-1 phosphorylation. EMBO J. 1995 Dec 1;14(23):5957-64.
REF 59 A novel c-Jun N-terminal kinase (JNK)-binding protein WDR62 is recruited to stress granules and mediates a nonclassical JNK activation. Mol Biol Cell. 2010 Jan 1;21(1):117-30.
REF 60 Dissociation of Akt1 from its negative regulator JIP1 is mediated through the ASK1-MEK-JNK signal transduction pathway during metabolic oxidative stress: a negative feedback loop. J Cell Biol. 2005 Jul 4;170(1):61-72.
REF 61 Ethanol rapidly causes activation of JNK associated with ER stress under inhibition of ADH. FEBS Lett. 2006 Jan 9;580(1):9-14.
REF 62 The c-Jun NH(2)-terminal kinase promotes insulin resistance during association with insulin receptor substrate-1 and phosphorylation of Ser(307). J Biol Chem. 2000 Mar 24;275(12):9047-54.
REF 63 Phosphorylation of Ser307 in insulin receptor substrate-1 blocks interactions with the insulin receptor and inhibits insulin action. J Biol Chem. 2002 Jan 11;277(2):1531-7.
REF 64 Cytoskeleton-mediated death receptor and ligand concentration in lipid rafts forms apoptosis-promoting clusters in cancer chemotherapy. J Biol Chem. 2005 Mar 25;280(12):11641-7.
REF 65 p21(Cip1) up-regulated during histone deacetylase inhibitor-induced CD4(+) T-cell anergy selectively associates with mitogen-activated protein kinases. Immunology. 2010 Apr;129(4):589-99.
REF 66 The stress-activated protein kinases p38 alpha and JNK1 stabilize p21(Cip1) by phosphorylation. J Biol Chem. 2002 Aug 16;277(33):29792-802.

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