Target Information

Target General Information

Target ID

T65239 (Former ID: TTDI03027)

Target Name

Ammonium transporter Rh type A (RHAG)

Synonyms

Rhesus blood group-associated glycoprotein; Rhesus blood group-associated ammonia channel; Rhesus blood group family type A glycoprotein; Rh50A; Rh type A glycoprotein; Rh family type A glycoprotein; RH50; Erythrocyte plasma membrane 50 kDa glycoprotein; Erythrocyte membrane glycoprotein Rh50; CD241

Click to Show/Hide

Gene Name

RHAG

Target Type

Literature-reported target

[1]

Function

Associated with rhesus blood group antigen expression. May be part of an oligomeric complex which is likely to have a transport or channel function in the erythrocyte membrane. Involved in ammonia transport across the erythrocyte membrane. Seems to act in monovalent cation transport.

Click to Show/Hide

UniProt ID

RHAG_HUMAN

Sequence

MRFTFPLMAIVLEIAMIVLFGLFVEYETDQTVLEQLNITKPTDMGIFFELYPLFQDVHVM
IFVGFGFLMTFLKKYGFSSVGINLLVAALGLQWGTIVQGILQSQGQKFNIGIKNMINADF
SAATVLISFGAVLGKTSPTQMLIMTILEIVFFAHNEYLVSEIFKASDIGASMTIHAFGAY
FGLAVAGILYRSGLRKGHENEESAYYSDLFAMIGTLFLWMFWPSFNSAIAEPGDKQCRAI
VNTYFSLAACVLTAFAFSSLVEHRGKLNMVHIQNATLAGGVAVGTCADMAIHPFGSMIIG
SIAGMVSVLGYKFLTPLFTTKLRIHDTCGVHNLHGLPGVVGGLAGIVAVAMGASNTSMAM
QAAALGSSIGTAVVGGLMTGLILKLPLWGQPSDQNCYDDSVYWKVPKTR

Click to Show/Hide

3D Structure

Click to Show 3D Structure of This Target

AlphaFold

Cell-based Target Expression Variations

Top

Cell-based Target Expression Variations

Cell-based Target Expression Variations Info

Drug Binding Sites of Target

Top

Ligand Name: Cholesterol

Ligand Info

Structure Description

Local refinement of RhAG-RhCE-ANK1(AR1-5), from consensus refinement of all classes

PDB:7UZQ

Method

Electron microscopy

Resolution

2.17 Å

Mutation

[2]

PDB Sequence

MRFTFPLMAI

¹⁰

VLEIAMIVLF

²⁰

GLFVEYFELY

⁵¹

PLFQDVHVMI

⁶¹

FVGFGFLMTF

⁷¹

LKKYGFSSVG

⁸¹

INLLVAALGL

⁹¹

QWGTIVQGIL

¹⁰¹

QSQGQKFNIG

¹¹¹

IKNMINADFS

¹²¹

AATVLISFGA

¹³¹

VLGKTSPTQM

¹⁴¹

LIMTILEIVF

¹⁵¹

FAHNEYLVSE

¹⁶¹

IFKASDIGAS

¹⁷¹

MTIHAFGAYF

¹⁸¹

GLAVAGILYR

¹⁹¹

SGLRKGHENE

²⁰¹

ESAYYSDLFA

²¹¹

MIGTLFLWMF

²²¹

WPSFNSAIAE

²³¹

PGDKQCRAIV

²⁴¹

NTYFSLAACV

²⁵¹

LTAFAFSSLV

²⁶¹

EHRGKLNMVH

²⁷¹

IQNATLAGGV

²⁸¹

AVGTCADMAI

²⁹¹

HPFGSMIIGS

³⁰¹

IAGMVSVLGY

³¹¹

KFLTPLFTTK

³²¹

LRIHDTCGVH

³³¹

NLHGLPGVVG

³⁴¹

GLAGIVAVAM

³⁵¹

GASNTSMAMQ

³⁶¹

AAALGSSIGT

³⁷¹

AVVGGLMTGL

³⁸¹

ILKLPLWGQP

³⁹¹

SDQNCYDDSV

⁴⁰¹

YWKVPKTR

Residue

Distance (Å)

ALA179

4.859

TYR180

2.431

PHE181

4.904

LEU183

2.287

ALA184

2.675

GLY187

2.380

ILE188

2.324

TYR190

2.544

CYS286

3.036

ALA290

3.416

ILE291

2.353

HIS292

2.534

PHE294

2.227

GLY295

3.097

ILE298

2.404

ILE299

3.003

ILE302

2.758

PHE318

3.483

Residue

Distance (Å)

LEU322

2.857

ARG323

2.303

ILE324

2.239

HIS331

4.957

VAL339

4.800

VAL340

2.275

LEU343

2.359

ALA344

2.364

GLY345

4.930

VAL347

2.279

ALA348

2.280

MET351

2.643

ALA353

2.475

ILE369

4.852

VAL373

2.249

LEU377

2.303

LYS384

3.928

Click to View More Binding Site Information of This Target and Ligand Pair

Click to View More Binding Site Information of This Target with Different Ligands

Different Human System Profiles of Target	Top
Human Similarity Proteins of target is determined by comparing the sequence similarity of all human proteins with the target based on BLAST. The similarity proteins for a target are defined as the proteins with E-value < 0.005 and outside the protein families of the target. A target that has fewer human similarity proteins outside its family is commonly regarded to possess a greater capacity to avoid undesired interactions and thus increase the possibility of finding successful drugs (Brief Bioinform, 21: 649-662, 2020). Human Tissue Distribution of target is determined from a proteomics study that quantified more than 12,000 genes across 32 normal human tissues. Tissue Specificity (TS) score was used to define the enrichment of target across tissues. The distribution of targets among different tissues or organs need to be taken into consideration when assessing the target druggability, as it is generally accepted that the wider the target distribution, the greater the concern over potential adverse effects (Nat Rev Drug Discov, 20: 64-81, 2021). Biological Network Descriptors of target is determined based on a human protein-protein interactions (PPI) network consisting of 9,309 proteins and 52,713 PPIs, which were with a high confidence score of ≥ 0.95 collected from STRING database. The network properties of targets based on protein-protein interactions (PPIs) have been widely adopted for the assessment of target’s druggability. Proteins with high node degree tend to have a high impact on network function through multiple interactions, while proteins with high betweenness centrality are regarded to be central for communication in interaction networks and regulate the flow of signaling information (Front Pharmacol, 9, 1245, 2018; Curr Opin Struct Biol. 44:134-142, 2017). Human Similarity Proteins Human Tissue Distribution Biological Network Descriptors

Different Human System Profiles of Target

Top

Human Similarity Proteins of target is determined by comparing the sequence similarity of all human proteins with the target based on BLAST. The similarity proteins for a target are defined as the proteins with E-value < 0.005 and outside the protein families of the target.

A target that has fewer human similarity proteins outside its family is commonly regarded to possess a greater capacity to avoid undesired interactions and thus increase the possibility of finding successful drugs (Brief Bioinform, 21: 649-662, 2020).

Human Tissue Distribution of target is determined from a proteomics study that quantified more than 12,000 genes across 32 normal human tissues. Tissue Specificity (TS) score was used to define the enrichment of target across tissues.

The distribution of targets among different tissues or organs need to be taken into consideration when assessing the target druggability, as it is generally accepted that the wider the target distribution, the greater the concern over potential adverse effects (Nat Rev Drug Discov, 20: 64-81, 2021).

Biological Network Descriptors of target is determined based on a human protein-protein interactions (PPI) network consisting of 9,309 proteins and 52,713 PPIs, which were with a high confidence score of ≥ 0.95 collected from STRING database.

The network properties of targets based on protein-protein interactions (PPIs) have been widely adopted for the assessment of target’s druggability. Proteins with high node degree tend to have a high impact on network function through multiple interactions, while proteins with high betweenness centrality are regarded to be central for communication in interaction networks and regulate the flow of signaling information (Front Pharmacol, 9, 1245, 2018; Curr Opin Struct Biol. 44:134-142, 2017).

Human Similarity Proteins

Human Tissue Distribution

Biological Network Descriptors

There is no similarity protein (E value < 0.005) for this target


Note: If a protein has TS (tissue specficity) scores at least in one tissue >= 2.5, this protein is called tissue-enriched (including tissue-enriched-but-not-specific and tissue-specific). In the plots, the vertical lines are at thresholds 2.5 and 4.

Degree	1	Degree centrality	1.07E-04	Betweenness centrality	0.00E+00
Closeness centrality	1.78E-01	Radiality	1.29E+01	Clustering coefficient	0.00E+00
Neighborhood connectivity	2.20E+01	Topological coefficient	1.00E+00	Eccentricity	12
Download	Click to Download the Full PPI Network of This Target

References

Top

REF 1

The Rh complex exports ammonium from human red blood cells. Br J Haematol. 2003 Jul;122(2):333-40.

REF 2

Architecture of the human erythrocyte ankyrin-1 complex. Nat Struct Mol Biol. 2022 Jul;29(7):706-718.

If You Find Any Error in Data or Bug in Web Service, Please Kindly Report It to Dr. Zhou and Dr. Zhang.

Prof. Feng ZHU

Prof. Yuzong CHEN