Target Interacting Protein Information

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Target General Information
Target Interacting Proteins
References
Target General Information  Top
Target ID T16452 Target Info
Target Name Multiple tumor suppressor 1 (CDKN2A)
Synonyms p16INK4A; Tumour suppressor gene p16; P16-INK4a; P16-INK4; P16 gene; MTS-1; Cyclin-dependent kinase inhibitor 2A; Cyclin-dependent kinase 4 inhibitor A; CDK4I
Target Type Literature-reported Target
Gene Name CDKN2A
UniProt ID
CDN2A_HUMAN 
Target Interacting Proteins  Top
Acyltransferases [+] 2 Acyltransferases +
Interacting Target Name E3 ubiquitin-protein ligase TRIP12 (TRIP12) Literature-reported Target Interacting Protein Info
UniProt ID
TRIPC_HUMAN 
Synonyms ULF; TRIP12; TRinteracting protein 12; Thyroid receptorinteracting protein 12; E3 ubiquitinprotein ligase TRIP12; E3 ubiquitinprotein ligase for Arf
Evidence Score (E-score) 6 Experimental Evidences +
1 Affinity Capture-Western [1]
2 Affinity Capture-Western [2]
3 Affinity Capture-Western [3]
4 Affinity Capture-Western [4]
5 Reconstituted Complex [1]
6 Reconstituted Complex [5]
Interacting Protein Name E3 ubiquitin-protein ligase HUWE1 (HUWE1) Interacting Protein Interacting Protein Info
UniProt ID
HUWE1_HUMAN 
Synonyms ARF.BP1; HECTH9; HSPC272; Ib772; LASU1; MULE; URE.B1; UREB1
Evidence Score (E-score) 4 Experimental Evidences +
1 Affinity Capture-MS [6]
2 Affinity Capture-Western [6]
3 Affinity Capture-Western [2]
4 Reconstituted Complex [6]
Cell cycle related proteins [+] 2 Cell cycle related proteins +
Interacting Protein Name G1/S-specific cyclin-D2 (CCND2) Interacting Protein Interacting Protein Info
UniProt ID
CCND2_HUMAN 
Synonyms KIAK0002; MPPH3
Evidence Score (E-score) 3 Experimental Evidences +
1 Fluorescence Resonance Energy Transfer [7]
2 Reconstituted Complex [8]
3 Reconstituted Complex [9]
Interacting Target Name G1/S-specific cyclin-D1 (CCND1) Clinical trial Target Interacting Protein Info
UniProt ID
CCND1_HUMAN 
Synonyms PRAD1 oncogene; PRAD1; Cyclin D1; BCL-1 oncogene; BCL-1; BCL1; B-cell lymphoma 1 protein
Evidence Score (E-score) 2 Experimental Evidences +
1 Affinity Capture-Western [10]
2 Reconstituted Complex [11]
Heat shock proteins [+] 1 Heat shock proteins +
Interacting Target Name Mortalin (HSPA9) Clinical trial Target Interacting Protein Info
UniProt ID
GRP75_HUMAN 
Synonyms Stress-70 protein, mitochondrial; Peptide-binding protein 74; PBP74; mt-HSP70; MOT; HSPA9B; Heat shock 70 kDa protein 9; GRP-75; GRP75; 75 kDa glucose-regulated protein
Evidence Score (E-score) 2 Experimental Evidences +
1 Affinity Capture-MS [12]
2 Reconstituted Complex [13]
Isomerases [+] 1 Isomerases +
Interacting Target Name DNA topoisomerase I (TOP1) Successful Target Interacting Protein Info
UniProt ID
TOP1_HUMAN 
Synonyms DNA topoisomerase I
Evidence Score (E-score) 3 Experimental Evidences +
1 Affinity Capture-Western [14]
2 Affinity Capture-Western [15]
3 Co-localization [15]
Kinases [+] 2 Kinases +
Interacting Target Name Cyclin-dependent kinase 4 (CDK4) Successful Target Interacting Protein Info
UniProt ID
CDK4_HUMAN 
Synonyms PSK-J3; Cell division protein kinase 4
Evidence Score (E-score) 12 Experimental Evidences +
1 Affinity Capture-Western [10]
2 Affinity Capture-Western [16]
3 Fluorescence Resonance Energy Transfer [7]
4 Protein-peptide [17]
5 Reconstituted Complex [8]
6 Reconstituted Complex [18]
7 Reconstituted Complex [19]
8 Reconstituted Complex [20]
9 Reconstituted Complex [9]
10 Reconstituted Complex [21]
11 Yeast two hybrid [22]
12 Yeast two hybrid [11]
Interacting Target Name Cyclin-dependent kinase 6 (CDK6) Successful Target Interacting Protein Info
UniProt ID
CDK6_HUMAN 
Synonyms Serine/threonine-protein kinase PLSTIRE; Serine/threonine protein kinase PLSTIRE; Cell division protein kinase 6; CDKN6
Evidence Score (E-score) 8 Experimental Evidences +
1 Affinity Capture-Western [23]
2 Fluorescence Resonance Energy Transfer [7]
3 Protein-peptide [17]
4 Reconstituted Complex [19]
5 Reconstituted Complex [18]
6 Reconstituted Complex [20]
7 Reconstituted Complex [21]
8 Reconstituted Complex [13]
Pore-forming toxins [+] 1 Pore-forming toxins +
Interacting Target Name Cellular tumor antigen p53 (TP53) Clinical trial Target Interacting Protein Info
UniProt ID
P53_HUMAN 
Synonyms Tumor suppressor p53; Phosphoprotein p53; P53; Antigen NY-CO-13
Evidence Score (E-score) 2 Experimental Evidences +
1 Affinity Capture-Western [24]
2 Fluorescence Resonance Energy Transfer [7]
Transcription regulators [+] 3 Transcription regulators +
Interacting Target Name Hypoxia-inducible factor 1 alpha (HIF-1A) Clinical trial Target Interacting Protein Info
UniProt ID
HIF1A_HUMAN 
Synonyms Transcription factor HIF-1; PASD8; PAS domain-containing protein 8; MOP1; Member of PAS protein 1; Hypoxia-inducible transcription factor (HIF)-1; Hypoxia-inducible factor 1-alpha; Hypoxia-inducible factor 1; Hypoxia inducible factor 1; HIF-1-alpha; HIF-1alpha; HIF1-alpha; HIF-1 alpha; HIF1 alpha; Class E basic helix-loop-helix protein 78; bHLHe78; Basic-helix-loop-helix-PAS protein MOP1; ARNT-interacting protein; ARNT interacting protein
Evidence Score (E-score) 2 Experimental Evidences +
1 Affinity Capture-Western [25]
2 Fluorescence Resonance Energy Transfer [7]
Interacting Target Name Proto-oncogene c-Myc (MYC) Literature-reported Target Interacting Protein Info
UniProt ID
MYC_HUMAN 
Synonyms Transcription factor p64; Proto-oncogene c-Myc; Myc proto-oncogene protein; C-myc oncogene; C-myc; Class E basic helix-loop-helix protein 39; bHLHe39
Evidence Score (E-score) 2 Experimental Evidences +
1 Affinity Capture-Western [26]
2 Fluorescence Resonance Energy Transfer [7]
Interacting Protein Name Zinc finger protein 420 (ZNF420) Interacting Protein Interacting Protein Info
UniProt ID
ZN420_HUMAN 
Synonyms APAK
Evidence Score (E-score) 2 Experimental Evidences +
1 Affinity Capture-Western [27]
2 Reconstituted Complex [28]
Ubiquitin related proteins [+] 1 Ubiquitin related proteins +
Interacting Target Name Ubiquitin-protein ligase E3 Mdm2 (MDM2) Clinical trial Target Interacting Protein Info
UniProt ID
MDM2_HUMAN 
Synonyms RING-type E3 ubiquitin transferase Mdm2; P53-binding protein Mdm2; Oncoprotein Mdm2; MDM2 protein; Hdm2; E3 ubiquitin-protein ligase Mdm2; Double minute 2 protein
Evidence Score (E-score) 20 Experimental Evidences +
1 Affinity Capture-Western [29]
2 Affinity Capture-Western [20]
3 Affinity Capture-Western [22]
4 Affinity Capture-Western [30]
5 Affinity Capture-Western [31]
6 Affinity Capture-Western [32]
7 Affinity Capture-Western [33]
8 Affinity Capture-Western [34]
9 Affinity Capture-Western [35]
10 Affinity Capture-Western [36]
11 Affinity Capture-Western [37]
12 Affinity Capture-Western [3]
13 Affinity Capture-Western [38]
14 Affinity Capture-Western [39]
15 Reconstituted Complex [40]
16 Reconstituted Complex [12]
17 Reconstituted Complex [22]
18 Reconstituted Complex [41]
19 Yeast two hybrid [42]
20 Yeast two hybrid [22]
Other proteins [+] 2 Other proteins +
Interacting Target Name Nucleophosmin (NPM1) Clinical trial Target Interacting Protein Info
UniProt ID
NPM_HUMAN 
Synonyms Numatrin; Nucleolar protein NO38; Nucleolar phosphoprotein B23; NPM
Evidence Score (E-score) 9 Experimental Evidences +
1 Affinity Capture-MS [6]
2 Affinity Capture-MS [43]
3 Affinity Capture-Western [44]
4 Affinity Capture-Western [6]
5 Affinity Capture-Western [2]
6 Affinity Capture-Western [43]
7 Affinity Capture-Western [45]
8 Reconstituted Complex [44]
9 Reconstituted Complex [46]
Interacting Target Name von Hippel-Lindau disease tumor suppressor (VHL) Patented-recorded Target Interacting Protein Info
UniProt ID
VHL_HUMAN 
Synonyms pVHL; Protein G7
Evidence Score (E-score) 3 Experimental Evidences +
1 Affinity Capture-MS [47]
2 Co-fractionation [48]
3 Yeast two hybrid [49]
References  Top
REF 1 Transcription-independent ARF regulation in oncogenic stress-mediated p53 responses. Nature. 2010 Mar 25;464(7288):624-7.
REF 2 TRADD contributes to tumour suppression by regulating ULF-dependent p19Arf ubiquitylation. Nat Cell Biol. 2012 May 6;14(6):625-33.
REF 3 Nucleostemin stabilizes ARF by inhibiting the ubiquitin ligase ULF. Oncogene. 2015 Mar 26;34(13):1688-97.
REF 4 GLTSCR2 promotes the nucleoplasmic translocation and subsequent degradation of nucleolar ARF. Oncotarget. 2017 Mar 7;8(10):16293-16302.
REF 5 NMI mediates transcription-independent ARF regulation in response to cellular stresses. Mol Biol Cell. 2012 Dec;23(23):4635-46.
REF 6 ARF-BP1/Mule is a critical mediator of the ARF tumor suppressor. Cell. 2005 Jul 1;121(7):1071-83.
REF 7 OncoPPi-informed discovery of mitogen-activated protein kinase kinase 3 as a novel binding partner of c-Myc. Oncogene. 2017 Oct 19;36(42):5852-5860.
REF 8 The nuclear protein p34SEI-1 regulates the kinase activity of cyclin-dependent kinase 4 in a concentration-dependent manner. Biochemistry. 2004 Apr 13;43(14):4394-9.
REF 9 Novel insights into the INK4-CDK4/6-Rb pathway: counter action of gankyrin against INK4 proteins regulates the CDK4-mediated phosphorylation of Rb. Biochemistry. 2002 Mar 26;41(12):3977-83.
REF 10 PCAF acts as a gastric cancer suppressor through a novel PCAF-p16-CDK4 axis. Am J Cancer Res. 2016 Dec 1;6(12):2772-2786.
REF 11 Regulation of CDK4 activity by a novel CDK4-binding protein, p34(SEI-1). Genes Dev. 1999 Nov 15;13(22):3027-33.
REF 12 ARF induces autophagy by virtue of interaction with Bcl-xl. J Biol Chem. 2009 Jan 30;284(5):2803-10.
REF 13 Proteomic analysis of p16ink4a-binding proteins. Proteomics. 2007 Nov;7(22):4102-11.
REF 14 Human Arf tumor suppressor specifically interacts with chromatin containing the promoter of rRNA genes. Oncogene. 2004 Oct 21;23(49):8097-104.
REF 15 Delineation of the domains required for physical and functional interaction of p14ARF with human topoisomerase I. Oncogene. 2003 Apr 3;22(13):1945-54.
REF 16 Activation of a cAMP pathway and induction of melanogenesis correlate with association of p16(INK4) and p27(KIP1) to CDKs, loss of E2F-binding activity, and premature senescence of human melanocytes. Exp Cell Res. 1999 Dec 15;253(2):561-72.
REF 17 Inhibition of pRb phosphorylation and cell-cycle progression by a 20-residue peptide derived from p16CDKN2/INK4A. Curr Biol. 1996 Jan 1;6(1):84-91.
REF 18 Self-assembling protein microarrays. Science. 2004 Jul 2;305(5680):86-90.
REF 19 Biologic and biochemical analyses of p16(INK4a) mutations from primary tumors. J Natl Cancer Inst. 1999 Sep 15;91(18):1569-74.
REF 20 A CDKN2A mutation in familial melanoma that abrogates binding of p16INK4a to CDK4 but not CDK6. Cancer Res. 2007 Oct 1;67(19):9134-41.
REF 21 CDKN2A and CDK4 mutation analysis in Italian melanoma-prone families: functional characterization of a novel CDKN2A germ line mutation. Br J Cancer. 2001 Sep 14;85(6):836-44.
REF 22 ARF promotes MDM2 degradation and stabilizes p53: ARF-INK4a locus deletion impairs both the Rb and p53 tumor suppression pathways. Cell. 1998 Mar 20;92(6):725-34.
REF 23 Hypo-phosphorylation of the retinoblastoma protein (pRb) by cyclin D:Cdk4/6 complexes results in active pRb. Proc Natl Acad Sci U S A. 1997 Sep 30;94(20):10699-704.
REF 24 Association of p14ARF with the p120E4F transcriptional repressor enhances cell cycle inhibition. J Biol Chem. 2003 Feb 14;278(7):4981-9.
REF 25 The p14ARF tumor suppressor protein facilitates nucleolar sequestration of hypoxia-inducible factor-1alpha (HIF-1alpha ) and inhibits HIF-1-mediated transcription. J Biol Chem. 2001 Jul 27;276(30):28421-9.
REF 26 Domain-specific c-Myc ubiquitylation controls c-Myc transcriptional and apoptotic activity. Proc Natl Acad Sci U S A. 2013 Jan 15;110(3):978-83.
REF 27 ARF-dependent regulation of ATM and p53 associated KZNF (Apak) protein activity in response to oncogenic stress. FEBS Lett. 2010 Sep 24;584(18):3909-15.
REF 28 ARF-mediated SUMOylation of Apak antagonizes ubiquitylation and promotes its nucleolar accumulation to inhibit 47S pre-rRNA synthesis. J Mol Cell Biol. 2015 Apr;7(2):154-67.
REF 29 Multiple interacting domains contribute to p14ARF mediated inhibition of MDM2. Oncogene. 2002 Jul 4;21(29):4498-507.
REF 30 Ribosomal protein L11 negatively regulates oncoprotein MDM2 and mediates a p53-dependent ribosomal-stress checkpoint pathway. Mol Cell Biol. 2003 Dec;23(23):8902-12.
REF 31 p14(ARF) inhibits the functions of adenovirus E1A oncoprotein. Biochem J. 2011 Mar 1;434(2):275-85.
REF 32 The tumor suppressor UCHL1 forms a complex with p53/MDM2/ARF to promote p53 signaling and is frequently silenced in nasopharyngeal carcinoma. Clin Cancer Res. 2010 Jun 1;16(11):2949-58.
REF 33 A novel ARF-binding protein (LZAP) alters ARF regulation of HDM2. Biochem J. 2006 Jan 15;393(Pt 2):489-501.
REF 34 MDM2 splice variants predominantly localize to the nucleoplasm mediated by a COOH-terminal nuclear localization signal. Mol Cancer Res. 2007 Apr;5(4):403-12.
REF 35 p14ARF is capable of promoting HIV-1 tat degradation. Cell Cycle. 2008 May 15;7(10):1433-9.
REF 36 Functional and physical interactions of the ARF tumor suppressor with p53 and Mdm2. Proc Natl Acad Sci U S A. 1998 Jul 7;95(14):8292-7.
REF 37 The alternative product from the human CDKN2A locus, p14(ARF), participates in a regulatory feedback loop with p53 and MDM2. EMBO J. 1998 Sep 1;17(17):5001-14.
REF 38 Hypophosphorylation of Mdm2 augments p53 stability. Mol Cell Biol. 2002 Sep;22(17):6170-82.
REF 39 Identification of XBP1-u as a novel regulator of the MDM2/p53 axis using an shRNA library. Sci Adv. 2017 Oct 18;3(10):e1701383.
REF 40 p14 Arf promotes small ubiquitin-like modifier conjugation of Werners helicase. J Biol Chem. 2004 Nov 26;279(48):50157-66.
REF 41 Peptides derived from two dynamically disordered proteins self-assemble into amyloid-like fibrils. J Am Chem Soc. 2003 Mar 19;125(11):3200-1.
REF 42 Two arginine rich domains in the p14ARF tumour suppressor mediate nucleolar localization. Oncogene. 2000 Jun 15;19(26):2978-85.
REF 43 Tumor suppressor ARF degrades B23, a nucleolar protein involved in ribosome biogenesis and cell proliferation. Mol Cell. 2003 Nov;12(5):1151-64.
REF 44 Essential role of the B23/NPM core domain in regulating ARF binding and B23 stability. J Biol Chem. 2006 Jul 7;281(27):18463-72.
REF 45 The Arf tumor suppressor protein inhibits Miz1 to suppress cell adhesion and induce apoptosis. J Cell Biol. 2010 Mar 22;188(6):905-18.
REF 46 Identification of a novel nucleophosmin-interaction motif in the tumor suppressor p14arf. FEBS J. 2018 Mar;285(5):832-847.
REF 47 Proteomic dissection of the von Hippel-Lindau (VHL) interactome. J Proteome Res. 2011 Nov 4;10(11):5175-82.
REF 48 Characterization of the VHL tumor suppressor gene product: localization, complex formation, and the effect of natural inactivating mutations. Proc Natl Acad Sci U S A. 1995 Jul 3;92(14):6459-63.
REF 49 Isoform-specific interactions of the von Hippel-Lindau tumor suppressor protein. Sci Rep. 2015 Jul 27;5:12605.

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