Target Interacting Protein Information

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Target General Information
Target Interacting Proteins
References
Target General Information  Top
Target ID T96311 Target Info
Target Name NEDD8-activating enzyme E1C (UBA3)
Synonyms Ubiquitin-like modifier-activating enzyme 3; Ubiquitin-activating enzyme E1C; Ubiquitin-activating enzyme 3; UBE1C; NEDD8-activating enzyme E1 catalytic subunit
Target Type Literature-reported Target
Gene Name UBA3
Biochemical Class Carbon-sulfur ligase
UniProt ID
UBA3_HUMAN 
Target Interacting Proteins  Top
Acyltransferases [+] 1 Acyltransferases +
Interacting Protein Name NEDD8-conjugating enzyme Ubc12 (UBE2M) Interacting Protein Interacting Protein Info
UniProt ID
UBC12_HUMAN 
Synonyms UBC.RS2; UBC12; hUbc12
Evidence Score (E-score) 7 Experimental Evidences +
1 Biochemical Activity [1]
2 Biochemical Activity [2]
3 Biochemical Activity [3]
4 Biochemical Activity [4]
5 Biochemical Activity [5]
6 Biochemical Activity [6]
7 Reconstituted Complex [6]
Ubiquitin related proteins [+] 1 Ubiquitin related proteins +
Interacting Protein Name NEDD8-activating enzyme E1 regulatory subunit (NAE1) Interacting Protein Interacting Protein Info
UniProt ID
ULA1_HUMAN 
Synonyms A.116A10.1; APPBP1; ula.1
Evidence Score (E-score) 5 Experimental Evidences +
1 Affinity Capture-Western [7]
2 Co-fractionation [8]
3 Co-fractionation [9]
4 Fluorescence Resonance Energy Transfer [3]
5 Reconstituted Complex [4]
References  Top
REF 1 A unique E1-E2 interaction required for optimal conjugation of the ubiquitin-like protein NEDD8. Nat Struct Mol Biol. 2004 Oct;11(10):927-35.
REF 2 E2-RING expansion of the NEDD8 cascade confers specificity to cullin modification. Mol Cell. 2009 Feb 27;33(4):483-95.
REF 3 Dissecting Distinct Roles of NEDDylation E1 Ligase Heterodimer APPBP1 and UBA3 Reveals Potential Evolution Process for Activation of Ubiquitin-related Pathways. Sci Rep. 2018 Jul 4;8(1):10108.
REF 4 Identification of the activating and conjugating enzymes of the NEDD8 conjugation pathway. J Biol Chem. 1999 Apr 23;274(17):12036-42.
REF 5 Neddylation requires glycyl-tRNA synthetase to protect activated E2. Nat Struct Mol Biol. 2016 Aug;23(8):730-7.
REF 6 Structural dissection of a gating mechanism preventing misactivation of ubiquitin by NEDD8's E1. Biochemistry. 2008 Aug 26;47(34):8961-9.
REF 7 The amyloid precursor protein-binding protein APP-BP1 drives the cell cycle through the S-M checkpoint and causes apoptosis in neurons. J Biol Chem. 2000 Mar 24;275(12):8929-35.
REF 8 A high-throughput approach for measuring temporal changes in the interactome. Nat Methods. 2012 Sep;9(9):907-9.
REF 9 A census of human soluble protein complexes. Cell. 2012 Aug 31;150(5):1068-81.

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