Target Interacting Protein Information

Content Navigation (  All   None )                                      
Target General Information
Target Interacting Proteins
References
Target General Information  Top
Target ID T88304 Target Info
Target Name DNA [cytosine-5]-methyltransferase 1 (DNMT1)
Synonyms MCMT; M.HsaI; Dnmt1; DNMT; DNA methyltransferase HsaI; DNA MTase HsaI; DNA (cytosine5)methyltransferase 1; DNA (cytosine-5)-methyltransferase 1; CXXCtype zinc finger protein 9; CXXC9; CXXC-type zinc finger protein 9; AIM
Target Type Clinical trial Target
Gene Name DNMT1
Biochemical Class Methyltransferase
UniProt ID
DNMT1_HUMAN 
Target Interacting Proteins  Top
Acid anhydride hydrolases [+] 1 Acid anhydride hydrolases +
Interacting Protein Name Chromodomain-helicase-DNA-binding protein 4 (CHD4) Interacting Protein Interacting Protein Info
UniProt ID
CHD4_HUMAN 
Synonyms CHD.4; Mi.2b; Mi2.BETA
Evidence Score (E-score) 4 Experimental Evidences +
1 Affinity Capture-MS [1]
2 Affinity Capture-Western [1]
3 Affinity Capture-Western [2]
4 Co-localization [1]
Acyltransferases [+] 1 Acyltransferases +
Interacting Protein Name E3 ubiquitin-protein ligase UHRF1 (UHRF1) Interacting Protein Interacting Protein Info
UniProt ID
UHRF1_HUMAN 
Synonyms ICBP90; Np95; RNF106; hNP95; hUHRF1; huNp95
Evidence Score (E-score) 12 Experimental Evidences +
1 Affinity Capture-Western [3]
2 Affinity Capture-Western [4]
3 Affinity Capture-Western [5]
4 Affinity Capture-Western [6]
5 Affinity Capture-Western [7]
6 Affinity Capture-Western [8]
7 Affinity Capture-Western [9]
8 Co-localization [10]
9 Co-localization [11]
10 Reconstituted Complex [5]
11 Reconstituted Complex [8]
12 Reconstituted Complex [12]
Carbon-nitrogen hydrolases [+] 3 Carbon-nitrogen hydrolases +
Interacting Target Name Histone deacetylase 1 (HDAC1) Successful Target Interacting Protein Info
UniProt ID
HDAC1_HUMAN 
Synonyms RPD3L1; HD1
Evidence Score (E-score) 10 Experimental Evidences +
1 Affinity Capture-Western [3]
2 Affinity Capture-Western [1]
3 Affinity Capture-Western [2]
4 Affinity Capture-Western [13]
5 Affinity Capture-Western [14]
6 Affinity Capture-Western [15]
7 Affinity Capture-Western [16]
8 Co-fractionation [17]
9 Co-localization [18]
10 Reconstituted Complex [19]
Interacting Target Name Histone deacetylase 2 (HDAC2) Clinical trial Target Interacting Protein Info
UniProt ID
HDAC2_HUMAN 
Synonyms HD2
Evidence Score (E-score) 4 Experimental Evidences +
1 Affinity Capture-Western [1]
2 Affinity Capture-Western [2]
3 Reconstituted Complex [19]
4 Yeast two hybrid [20]
Interacting Target Name NAD-dependent deacetylase sirtuin-1 (SIRT1) Clinical trial Target Interacting Protein Info
UniProt ID
SIR1_HUMAN 
Synonyms SIR2-like protein 1; SIR2L1; Regulatory protein SIR2 homolog 1; NAD-dependent protein deacetylase sirtuin-1; hSIRT1; hSIR2
Evidence Score (E-score) 2 Experimental Evidences +
1 Affinity Capture-Western [21]
2 Biochemical Activity [21]
Cell division cycle proteins [+] 1 Cell division cycle proteins +
Interacting Protein Name Retinoblastoma-associated protein (RB1) Interacting Protein Interacting Protein Info
UniProt ID
RB_HUMAN 
Synonyms OSRC; PPP1R130; RB; p105.Rb; pRb; pp110
Evidence Score (E-score) 2 Experimental Evidences +
1 Affinity Capture-Western [22]
2 Co-fractionation [17]
One-carbon transferases [+] 4 One-carbon transferases +
Interacting Target Name DNA [cytosine-5]-methyltransferase 3B (DNMT3B) Clinical trial Target Interacting Protein Info
UniProt ID
DNM3B_HUMAN 
Synonyms M.HsaIIIB; Dnmt3b; DNA MTase HsaIIIB; DNA methyltransferase HsaIIIB; DNA methyltransferase 3B; DNA (cytosine-5)-methyltransferase 3B
Evidence Score (E-score) 4 Experimental Evidences +
1 Affinity Capture-MS [23]
2 Affinity Capture-Western [24]
3 Affinity Capture-Western [25]
4 Reconstituted Complex [25]
Interacting Target Name Enhancer of zeste homolog 2 (EZH2) Successful Target Interacting Protein Info
UniProt ID
EZH2_HUMAN 
Synonyms Lysine N-methyltransferase 6; Lysine Nmethyltransferase 6; KMT6; Histone-lysine N-methyltransferase EZH2; Histonelysine Nmethyltransferase EZH2; EZH2 Y641N mutations; EZH2 Y641F mutations; ENX-1; ENX1
Evidence Score (E-score) 3 Experimental Evidences +
1 Affinity Capture-MS [26]
2 Affinity Capture-Western [27]
3 Reconstituted Complex [27]
Interacting Target Name Histone-lysine N-methyltransferase SETD7 (SETD7) Literature-reported Target Interacting Protein Info
UniProt ID
SETD7_HUMAN 
Synonyms SET9; SET7/9; SET7; SET domain-containing protein 7; Lysine N-methyltransferase 7; KMT7; KIAA1717; Histone H3-K4 methyltransferase SETD7; H3-K4-HMTase SETD7
Evidence Score (E-score) 3 Experimental Evidences +
1 Affinity Capture-Western [28]
2 Biochemical Activity [28]
3 Co-crystal Structure [29]
Interacting Target Name Histone-lysine N-methyltransferase EHMT2 (EHMT2) Preclinical Target Interacting Protein Info
UniProt ID
EHMT2_HUMAN 
Synonyms Protein G9a; NG36; Lysine N-methyltransferase 1C; KMT1C; HLA-B-associated transcript 8; Histone H3-K9 methyltransferase 3; H3-K9-HMTase 3; G9A; Euchromatic histone-lysine N-methyltransferase 2; C6orf30; BAT8
Evidence Score (E-score) 2 Experimental Evidences +
1 Affinity Capture-MS [1]
2 Affinity Capture-Western [30]
Peptidases [+] 1 Peptidases +
Interacting Target Name Herpesvirus ubiquitin-specific protease (HAUSP) Preclinical Target Interacting Protein Info
UniProt ID
UBP7_HUMAN 
Synonyms Ubiquitin-specific-processing protease 7; Ubiquitin thioesterase 7; Ubiquitin carboxyl-terminal hydrolase 7; Herpesvirus-associated ubiquitin-specific protease; Deubiquitinating enzyme 7
Evidence Score (E-score) 11 Experimental Evidences +
1 Affinity Capture-MS [5]
2 Affinity Capture-MS [6]
3 Affinity Capture-Western [5]
4 Affinity Capture-Western [6]
5 Affinity Capture-Western [31]
6 Affinity Capture-Western [32]
7 Affinity Capture-Western [33]
8 Biochemical Activity [31]
9 Co-crystal Structure [33]
10 Reconstituted Complex [5]
11 Reconstituted Complex [33]
Transcription regulators [+] 1 Transcription regulators +
Interacting Target Name Metastasis associated gene-1 (MTA1) Literature-reported Target Interacting Protein Info
UniProt ID
MTA1_HUMAN 
Synonyms Metastasis-associated protein MTA1; Metastasisassociated protein MTA1
Evidence Score (E-score) 3 Experimental Evidences +
1 Affinity Capture-Western [1]
2 Affinity Capture-Western [2]
3 Co-fractionation [34]
Other proteins [+] 2 Other proteins +
Interacting Target Name Proliferating cell nuclear antigen (PCNA) Clinical trial Target Interacting Protein Info
UniProt ID
PCNA_HUMAN 
Synonyms Cyclin
Evidence Score (E-score) 7 Experimental Evidences +
1 Affinity Capture-MS [1]
2 Affinity Capture-Western [35]
3 Affinity Capture-Western [36]
4 Affinity Capture-Western [4]
5 Protein-peptide [36]
6 Reconstituted Complex [37]
7 Reconstituted Complex [36]
Interacting Protein Name Metastasis-associated protein MTA2 (MTA2) Interacting Protein Interacting Protein Info
UniProt ID
MTA2_HUMAN 
Synonyms MTA1L1; PID
Evidence Score (E-score) 3 Experimental Evidences +
1 Affinity Capture-MS [1]
2 Affinity Capture-Western [1]
3 Affinity Capture-Western [2]
References  Top
REF 1 The NuRD complex cooperates with DNMTs to maintain silencing of key colorectal tumor suppressor genes. Oncogene. 2014 Apr 24;33(17):2157-68.
REF 2 Nuclear receptors TR2 and TR4 recruit multiple epigenetic transcriptional corepressors that associate specifically with the embryonic -type globin promoters in differentiated adult erythroid cells. Mol Cell Biol. 2011 Aug;31(16):3298-311.
REF 3 The interaction of the SRA domain of ICBP90 with a novel domain of DNMT1 is involved in the regulation of VEGF gene expression. Oncogene. 2008 Apr 3;27(15):2187-97.
REF 4 Disruption of Dnmt1/PCNA/UHRF1 interactions promotes tumorigenesis from human and mice glial cells. PLoS One. 2010 Jun 29;5(6):e11333.
REF 5 The USP7/Dnmt1 complex stimulates the DNA methylation activity of Dnmt1 and regulates the stability of UHRF1. Nucleic Acids Res. 2011 Oct;39(19):8355-65.
REF 6 DNMT1 stability is regulated by proteins coordinating deubiquitination and acetylation-driven ubiquitination. Sci Signal. 2010 Nov 2;3(146):ra80.
REF 7 S phase-dependent interaction with DNMT1 dictates the role of UHRF1 but not UHRF2 in DNA methylation maintenance. Cell Res. 2011 Dec;21(12):1723-39.
REF 8 UHRF1 plays a role in maintaining DNA methylation in mammalian cells. Science. 2007 Sep 21;317(5845):1760-4.
REF 9 DNA methylation requires a DNMT1 ubiquitin interacting motif (UIM) and histone ubiquitination. Cell Res. 2015 Aug;25(8):911-29.
REF 10 UHRF1 is associated with epigenetic silencing of BRCA1 in sporadic breast cancer. Breast Cancer Res Treat. 2010 Sep;123(2):359-73.
REF 11 Tandem virtual screening targeting the SRA domain of UHRF1 identifies a novel chemical tool modulating DNA methylation. Eur J Med Chem. 2016 May 23;114:390-6.
REF 12 Hemi-methylated DNA opens a closed conformation of UHRF1 to facilitate its histone recognition. Nat Commun. 2016 Apr 5;7:11197.
REF 13 Methyl-CpG-binding protein, MeCP2, is a target molecule for maintenance DNA methyltransferase, Dnmt1. J Biol Chem. 2003 Feb 14;278(7):4806-12.
REF 14 STAT3- and DNA methyltransferase 1-mediated epigenetic silencing of SHP-1 tyrosine phosphatase tumor suppressor gene in malignant T lymphocytes. Proc Natl Acad Sci U S A. 2005 May 10;102(19):6948-53.
REF 15 Epigenetic changes induced by oxidative stress in colorectal cancer cells: methylation of tumor suppressor RUNX3. Tumour Biol. 2012 Apr;33(2):403-12.
REF 16 HBV-induced ROS accumulation promotes hepatocarcinogenesis through Snail-mediated epigenetic silencing of SOCS3. Cell Death Differ. 2016 Apr;23(4):616-27.
REF 17 DNMT1 forms a complex with Rb, E2F1 and HDAC1 and represses transcription from E2F-responsive promoters. Nat Genet. 2000 Jul;25(3):338-42.
REF 18 HDAC1 bound to the Cyp1a1 promoter blocks histone acetylation associated with Ah receptor-mediated trans-activation. Biochim Biophys Acta. 2007 Sep-Oct;1769(9-10):569-78.
REF 19 LSH cooperates with DNA methyltransferases to repress transcription. Mol Cell Biol. 2008 Jan;28(1):215-26.
REF 20 DNMT1 binds HDAC2 and a new co-repressor, DMAP1, to form a complex at replication foci. Nat Genet. 2000 Jul;25(3):269-77.
REF 21 SIRT1 deacetylates the DNA methyltransferase 1 (DNMT1) protein and alters its activities. Mol Cell Biol. 2011 Dec;31(23):4720-34.
REF 22 The retinoblastoma gene product interacts with maintenance human DNA (cytosine-5) methyltransferase and modulates its activity. EMBO J. 2002 Feb 15;21(4):779-88.
REF 23 System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation. Sci Signal. 2011 Mar 15;4(164):rs3.
REF 24 Suv39h-mediated histone H3 lysine 9 methylation directs DNA methylation to major satellite repeats at pericentric heterochromatin. Curr Biol. 2003 Jul 15;13(14):1192-200.
REF 25 Co-operation and communication between the human maintenance and de novo DNA (cytosine-5) methyltransferases. EMBO J. 2002 Aug 1;21(15):4183-95.
REF 26 TRIM28 interacts with EZH2 and SWI/SNF to activate genes that promote mammosphere formation. Oncogene. 2017 May 25;36(21):2991-3001.
REF 27 The Polycomb group protein EZH2 directly controls DNA methylation. Nature. 2006 Feb 16;439(7078):871-4.
REF 28 Regulation of DNMT1 stability through SET7-mediated lysine methylation in mammalian cells. Proc Natl Acad Sci U S A. 2009 Mar 31;106(13):5076-81.
REF 29 A methylation and phosphorylation switch between an adjacent lysine and serine determines human DNMT1 stability. Nat Struct Mol Biol. 2011 Jan;18(1):42-8.
REF 30 Direct interaction between DNMT1 and G9a coordinates DNA and histone methylation during replication. Genes Dev. 2006 Nov 15;20(22):3089-103.
REF 31 Usp7 and Uhrf1 control ubiquitination and stability of the maintenance DNA methyltransferase Dnmt1. J Cell Biochem. 2011 Feb;112(2):439-44.
REF 32 M phase phosphorylation of the epigenetic regulator UHRF1 regulates its physical association with the deubiquitylase USP7 and stability. Proc Natl Acad Sci U S A. 2012 Mar 27;109(13):4828-33.
REF 33 Molecular mechanism for USP7-mediated DNMT1 stabilization by acetylation. Nat Commun. 2015 May 11;6:7023.
REF 34 Panorama of ancient metazoan macromolecular complexes. Nature. 2015 Sep 17;525(7569):339-44.
REF 35 Rapid and transient recruitment of DNMT1 to DNA double-strand breaks is mediated by its interaction with multiple components of the DNA damage response machinery. Hum Mol Genet. 2011 Jan 1;20(1):126-40.
REF 36 Versatile toolbox for high throughput biochemical and functional studies with fluorescent fusion proteins. PLoS One. 2012;7(5):e36967.
REF 37 PCNA clamp facilitates action of DNA cytosine methyltransferase 1 on hemimethylated DNA. Genes Cells. 2002 Oct;7(10):997-1007.

If You Find Any Error in Data or Bug in Web Service, Please Kindly Report It to Dr. Zhou and Dr. Zhang.