Target Interacting Protein Information

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Target General Information
Target Interacting Proteins
References
Target General Information  Top
Target ID T49898 Target Info
Target Name Cyclin-dependent kinase 1 (CDK1)
Synonyms P34CDC2; P34 protein kinase; CDKN1; CDC28A; CDC2
Target Type Clinical trial Target
Gene Name CDK1
Biochemical Class Kinase
UniProt ID
CDK1_HUMAN 
Target Interacting Proteins  Top
Adapter proteins [+] 1 Adapter proteins +
Interacting Target Name Sequestosome-1 p62 (SQSTM1) Literature-reported Target Interacting Protein Info
UniProt ID
SQSTM_HUMAN 
Synonyms Ubiquitin-binding protein p62; Sequestosome-1; Phosphotyrosine-independent ligand for the Lck SH2 domain of 62kDa; Phosphotyrosine-independent ligand for the Lck SH2 domain of 62 kDa; OSIL; ORCA; EBIAP; EBI3-associated protein of 60 kDa
Evidence Score (E-score) 4 Experimental Evidences +
1 Affinity Capture-Western [1]
2 Affinity Capture-Western [2]
3 Biochemical Activity [1]
4 Reconstituted Complex [1]
Cell cycle related proteins [+] 3 Cell cycle related proteins +
Interacting Target Name Cyclin A2 (CCNA2) Literature-reported Target Interacting Protein Info
UniProt ID
CCNA2_HUMAN 
Synonyms Cyclin-A2; Cyclin-A; Cyclin A; CCNA
Evidence Score (E-score) 3 Experimental Evidences +
1 Affinity Capture-MS [3]
2 Affinity Capture-MS [4]
3 Affinity Capture-Western [5]
Interacting Target Name G1/S-specific cyclin-D1 (CCND1) Clinical trial Target Interacting Protein Info
UniProt ID
CCND1_HUMAN 
Synonyms PRAD1 oncogene; PRAD1; Cyclin D1; BCL-1 oncogene; BCL-1; BCL1; B-cell lymphoma 1 protein
Evidence Score (E-score) 2 Experimental Evidences +
1 Affinity Capture-MS [6]
2 Affinity Capture-Western [7]
Interacting Target Name G1/S-specific cyclin-E1 (CCNE1) Literature-reported Target Interacting Protein Info
UniProt ID
CCNE1_HUMAN 
Synonyms G1/S-specific cyclin E; Cyclin E; CCNE
Evidence Score (E-score) 2 Experimental Evidences +
1 Affinity Capture-Western [8]
2 Co-purification [8]
Ester hydrolases [+] 2 Ester hydrolases +
Interacting Protein Name Cyclin-dependent kinase inhibitor 3 (CDKN3) Interacting Protein Interacting Protein Info
UniProt ID
CDKN3_HUMAN 
Synonyms CDI1; CIP2; KAP; KAP1
Evidence Score (E-score) 3 Experimental Evidences +
1 Affinity Capture-MS [9]
2 Affinity Capture-Western [10]
3 Yeast two hybrid [10]
Interacting Target Name M-phase inducer phosphatase 3 (MPIP3) Literature-reported Target Interacting Protein Info
UniProt ID
MPIP3_HUMAN 
Synonyms Dual specificity phosphatase Cdc25C; Cdc25C phosphatase
Evidence Score (E-score) 2 Experimental Evidences +
1 Affinity Capture-MS [11]
2 Reconstituted Complex [12]
Histones [+] 1 Histones +
Interacting Protein Name Histone H1.1 (HIST1H1A) Interacting Protein Interacting Protein Info
UniProt ID
H11_HUMAN 
Synonyms H1.1; H1A; H1F1; HIST1
Evidence Score (E-score) 13 Experimental Evidences +
1 Affinity Capture-Western [13]
2 Biochemical Activity [14]
3 Biochemical Activity [15]
4 Biochemical Activity [16]
5 Biochemical Activity [17]
6 Biochemical Activity [18]
7 Biochemical Activity [1]
8 Biochemical Activity [19]
9 Biochemical Activity [20]
10 Biochemical Activity [21]
11 Biochemical Activity [22]
12 Biochemical Activity [23]
13 Biochemical Activity [24]
Kinases [+] 2 Kinases +
Interacting Target Name Epidermal growth factor receptor (EGFR) Successful Target Interacting Protein Info
UniProt ID
EGFR_HUMAN 
Synonyms Epidermal growth factor receptor variant III; Proto-oncogene c-ErbB-1 variant III; Receptor tyrosine-protein kinase erbB-1 variant III; EGFR variant III; ERBB variant III; ERBB1 variant III; HER1 variant III
Evidence Score (E-score) 3 Experimental Evidences +
1 Affinity Capture-MS [25]
2 Protein-Fragment Complementation Assay [26]
3 Yeast two hybrid [27]
Interacting Target Name Tyrosine-protein kinase Lyn (JTK8) Clinical trial Target Interacting Protein Info
UniProt ID
LYN_HUMAN 
Synonyms V-yes-1 Yamaguchi sarcoma viral related oncogene homolog; p56Lyn; p53Lyn; Lck/Yes-related novel protein tyrosine kinase
Evidence Score (E-score) 2 Experimental Evidences +
1 Affinity Capture-Western [28]
2 Biochemical Activity [28]
Membrane-bounded channels [+] 2 Membrane-bounded channels +
Interacting Target Name G2/mitotic-specific cyclin B1 (CCNB1) Patented-recorded Target Interacting Protein Info
UniProt ID
CCNB1_HUMAN 
Synonyms G2/mitotic-specific cyclin-B1; Cyclin B1; CCNB
Evidence Score (E-score) 17 Experimental Evidences +
1 Affinity Capture-MS [6]
2 Affinity Capture-MS [4]
3 Affinity Capture-MS [9]
4 Affinity Capture-MS [29]
5 Affinity Capture-MS [3]
6 Affinity Capture-Western [30]
7 Affinity Capture-Western [31]
8 Affinity Capture-Western [32]
9 Affinity Capture-Western [33]
10 Affinity Capture-Western [1]
11 Biochemical Activity [34]
12 Biochemical Activity [19]
13 Co-fractionation [35]
14 Co-fractionation [19]
15 Co-purification [36]
16 Co-purification [17]
17 Reconstituted Complex [37]
Interacting Protein Name G2/mitotic-specific cyclin-B2 (CCNB2) Interacting Protein Interacting Protein Info
UniProt ID
CCNB2_HUMAN 
Synonyms HsT17299
Evidence Score (E-score) 6 Experimental Evidences +
1 Affinity Capture-MS [6]
2 Affinity Capture-MS [4]
3 Affinity Capture-MS [9]
4 Affinity Capture-MS [29]
5 Affinity Capture-MS [3]
6 Co-fractionation [35]
One-carbon transferases [+] 1 One-carbon transferases +
Interacting Target Name Enhancer of zeste homolog 2 (EZH2) Successful Target Interacting Protein Info
UniProt ID
EZH2_HUMAN 
Synonyms Lysine N-methyltransferase 6; Lysine Nmethyltransferase 6; KMT6; Histone-lysine N-methyltransferase EZH2; Histonelysine Nmethyltransferase EZH2; EZH2 Y641N mutations; EZH2 Y641F mutations; ENX-1; ENX1
Evidence Score (E-score) 7 Experimental Evidences +
1 Affinity Capture-Western [38]
2 Affinity Capture-Western [39]
3 Biochemical Activity [40]
4 Biochemical Activity [39]
5 Biochemical Activity [38]
6 Biochemical Activity [41]
7 Biochemical Activity [42]
Pore-forming toxins [+] 1 Pore-forming toxins +
Interacting Target Name Cellular tumor antigen p53 (TP53) Clinical trial Target Interacting Protein Info
UniProt ID
P53_HUMAN 
Synonyms Tumor suppressor p53; Phosphoprotein p53; P53; Antigen NY-CO-13
Evidence Score (E-score) 2 Experimental Evidences +
1 Affinity Capture-Western [43]
2 Biochemical Activity [44]
Transcription regulators [+] 2 Transcription regulators +
Interacting Target Name Transcription factor Sp1 (SP1) Clinical trial Target Interacting Protein Info
UniProt ID
SP1_HUMAN 
Synonyms TSFP1
Evidence Score (E-score) 3 Experimental Evidences +
1 Affinity Capture-Western [45]
2 Biochemical Activity [45]
3 Biochemical Activity [46]
Interacting Target Name Hypoxia-inducible factor 1 alpha (HIF-1A) Clinical trial Target Interacting Protein Info
UniProt ID
HIF1A_HUMAN 
Synonyms Transcription factor HIF-1; PASD8; PAS domain-containing protein 8; MOP1; Member of PAS protein 1; Hypoxia-inducible transcription factor (HIF)-1; Hypoxia-inducible factor 1-alpha; Hypoxia-inducible factor 1; Hypoxia inducible factor 1; HIF-1-alpha; HIF-1alpha; HIF1-alpha; HIF-1 alpha; HIF1 alpha; Class E basic helix-loop-helix protein 78; bHLHe78; Basic-helix-loop-helix-PAS protein MOP1; ARNT-interacting protein; ARNT interacting protein
Evidence Score (E-score) 2 Experimental Evidences +
1 Affinity Capture-MS [47]
2 Affinity Capture-Western [48]
Other proteins [+] 5 Other proteins +
Interacting Protein Name Cyclin-dependent kinases regulatory subunit 1 (CKS1B) Interacting Protein Interacting Protein Info
UniProt ID
CKS1_HUMAN 
Synonyms CKS1; PNAS.16; PNAS.18; ckshs1
Evidence Score (E-score) 9 Experimental Evidences +
1 Affinity Capture-MS [9]
2 Affinity Capture-MS [4]
3 Affinity Capture-MS [29]
4 Affinity Capture-MS [3]
5 Affinity Capture-Western [49]
6 Affinity Capture-Western [50]
7 Co-fractionation [35]
8 Co-fractionation [51]
9 Yeast two hybrid [52]
Interacting Target Name Melanoma differentiation-associated protein 6 (CDKN1A) Literature-reported Target Interacting Protein Info
UniProt ID
CDN1A_HUMAN 
Synonyms WAF1; SDI1; PIC1; P21(WAF1); P21; Melanoma differentiation associated protein 6; MDA-6; MDA6; Cyclin-dependent kinase inhibitor 1; CIP1; CDK-interacting protein 1; CAP20
Evidence Score (E-score) 5 Experimental Evidences +
1 Affinity Capture-MS [6]
2 Affinity Capture-MS [9]
3 Affinity Capture-MS [3]
4 Affinity Capture-Western [53]
5 Affinity Capture-Western [5]
Interacting Protein Name Cyclin-dependent kinases regulatory subunit 2 (CKS2) Interacting Protein Interacting Protein Info
UniProt ID
CKS2_HUMAN 
Synonyms CKSHS2
Evidence Score (E-score) 5 Experimental Evidences +
1 Affinity Capture-MS [9]
2 Affinity Capture-MS [4]
3 Affinity Capture-MS [29]
4 Affinity Capture-MS [3]
5 Affinity Capture-Western [49]
Interacting Protein Name DNA damage-inducible transcript 1 protein (GADD45A) Interacting Protein Interacting Protein Info
UniProt ID
GA45A_HUMAN 
Synonyms DDIT1; GADD45
Evidence Score (E-score) 5 Experimental Evidences +
1 Affinity Capture-Western [54]
2 Affinity Capture-Western [14]
3 Affinity Capture-Western [30]
4 Reconstituted Complex [54]
5 Reconstituted Complex [14]
Interacting Target Name CDK inhibitor 1B p27Kip1 (CDKN1B) Literature-reported Target Interacting Protein Info
UniProt ID
CDN1B_HUMAN 
Synonyms p27Kip1; KIP1; Cyclin-dependent kinase inhibitor p27; Cyclindependent kinase inhibitor p27; Cyclin-dependent kinase inhibitor 1B
Evidence Score (E-score) 4 Experimental Evidences +
1 Affinity Capture-MS [6]
2 Affinity Capture-MS [9]
3 Affinity Capture-MS [3]
4 Affinity Capture-Western [55]
References  Top
REF 1 Phosphorylation of p62 by cdk1 controls the timely transit of cells through mitosis and tumor cell proliferation. Mol Cell Biol. 2011 Jan;31(1):105-17.
REF 2 Both p62/SQSTM1-HDAC6-dependent autophagy and the aggresome pathway mediate CDK1 degradation in human breast cancer. Sci Rep. 2017 Aug 30;7(1):10078.
REF 3 The protein interaction landscape of the human CMGC kinase group. Cell Rep. 2013 Apr 25;3(4):1306-20.
REF 4 Integrative analysis of kinase networks in TRAIL-induced apoptosis provides a source of potential targets for combination therapy. Sci Signal. 2015 Apr 7;8(371):rs3.
REF 5 Destabilization of CARP mRNAs by aloe-emodin contributes to caspase-8-mediated p53-independent apoptosis of human carcinoma cells. J Cell Biochem. 2011 Apr;112(4):1176-91.
REF 6 Architecture of the human interactome defines protein communities and disease networks. Nature. 2017 May 25;545(7655):505-509.
REF 7 Proliferating cell nuclear antigen and p21 are components of multiple cell cycle kinase complexes. Mol Biol Cell. 1993 Sep;4(9):897-906.
REF 8 Formation and activation of a cyclin E-cdk2 complex during the G1 phase of the human cell cycle. Science. 1992 Sep 18;257(5077):1689-94.
REF 9 The BioPlex Network: A Systematic Exploration of the Human Interactome. Cell. 2015 Jul 16;162(2):425-440.
REF 10 KAP: a dual specificity phosphatase that interacts with cyclin-dependent kinases. Proc Natl Acad Sci U S A. 1994 Mar 1;91(5):1731-5.
REF 11 Phenotypic and Interaction Profiling of the Human Phosphatases Identifies Diverse Mitotic Regulators. Cell Rep. 2016 Nov 22;17(9):2488-2501.
REF 12 An essential phosphorylation-site domain of human cdc25C interacts with both 14-3-3 and cyclins. J Biol Chem. 2000 Sep 15;275(37):28849-57.
REF 13 RGC-32 increases p34CDC2 kinase activity and entry of aortic smooth muscle cells into S-phase. J Biol Chem. 2002 Jan 4;277(1):502-8.
REF 14 Association with Cdc2 and inhibition of Cdc2/Cyclin B1 kinase activity by the p53-regulated protein Gadd45. Oncogene. 1999 May 6;18(18):2892-900.
REF 15 The cyclin box and C-terminus of cyclins A and E specify CDK activation and substrate specificity. Oncogene. 1997 Jan 30;14(4):491-8.
REF 16 PRC1: a human mitotic spindle-associated CDK substrate protein required for cytokinesis. Mol Cell. 1998 Dec;2(6):877-85.
REF 17 Reconstitution of cyclin-dependent cdc2 and cdk2 kinase activities in vitro. J Biol Chem. 1993 Sep 25;268(27):20433-42.
REF 18 BRCA1 regulates the G2/M checkpoint by activating Chk1 kinase upon DNA damage. Nat Genet. 2002 Mar;30(3):285-9.
REF 19 Characterization of the in vitro reconstituted cyclin A or B1-dependent cdk2 and cdc2 kinase activities. J Biol Chem. 1993 Sep 25;268(27):20443-51.
REF 20 Cell cycle regulation of the activity and subcellular localization of Plk1, a human protein kinase implicated in mitotic spindle function. J Cell Biol. 1995 Jun;129(6):1617-28.
REF 21 Hip2 ubiquitin-conjugating enzyme overcomes radiation-induced G2/M arrest. Biochim Biophys Acta. 2013 Dec;1833(12):2911-2921.
REF 22 Phosphorylation regulates SIRT1 function. PLoS One. 2008;3(12):e4020.
REF 23 The anaphase-promoting complex or cyclosome supports cell survival in response to endoplasmic reticulum stress. PLoS One. 2012;7(4):e35520.
REF 24 Phosphorylation of Plk1 at Ser326 regulates its functions during mitotic progression. Oncogene. 2008 Nov 6;27(52):6635-45.
REF 25 Tandem immunoprecipitation of phosphotyrosine-mass spectrometry (TIPY-MS) indicates C19ORF19 becomes tyrosine-phosphorylated and associated with activated epidermal growth factor receptor. J Proteome Res. 2008 Mar;7(3):1067-77.
REF 26 The mammalian-membrane two-hybrid assay (MaMTH) for probing membrane-protein interactions in human cells. Nat Methods. 2014 May;11(5):585-92.
REF 27 In silico prediction of physical protein interactions and characterization of interactome orphans. Nat Methods. 2015 Jan;12(1):79-84.
REF 28 Activation of Src-like p56/p53lyn tyrosine kinase by ionizing radiation. J Biol Chem. 1994 Aug 12;269(32):20739-43.
REF 29 A human interactome in three quantitative dimensions organized by stoichiometries and abundances. Cell. 2015 Oct 22;163(3):712-23.
REF 30 Identification of a functional domain in a GADD45-mediated G2/M checkpoint. J Biol Chem. 2000 Nov 24;275(47):36892-8.
REF 31 Cyclin F regulates the nuclear localization of cyclin B1 through a cyclin-cyclin interaction. EMBO J. 2000 Mar 15;19(6):1378-88.
REF 32 Bortezomib potentially inhibits cellular growth of vascular endothelial cells through suppression of G2/M transition. Cancer Sci. 2010 Jun;101(6):1403-8.
REF 33 cdc2-cyclin B regulates eEF2 kinase activity in a cell cycle- and amino acid-dependent manner. EMBO J. 2008 Apr 9;27(7):1005-16.
REF 34 A novel RING finger protein, human enhancer of invasion 10, alters mitotic progression through regulation of cyclin B levels. Mol Cell Biol. 2003 Mar;23(6):2109-22.
REF 35 Panorama of ancient metazoan macromolecular complexes. Nature. 2015 Sep 17;525(7569):339-44.
REF 36 Response gene to complement 32 is required for C5b-9 induced cell cycle activation in endothelial cells. Exp Mol Pathol. 2009 Apr;86(2):87-94.
REF 37 Effects of phosphorylation by CAK on cyclin binding by CDC2 and CDK2. Mol Cell Biol. 1995 Jan;15(1):345-50.
REF 38 CDK1-dependent phosphorylation of EZH2 suppresses methylation of H3K27 and promotes osteogenic differentiation of human mesenchymal stem cells. Nat Cell Biol. 2011 Jan;13(1):87-94.
REF 39 Fine-tuning the stimulation of MLL1 methyltransferase activity by a histone H3-based peptide mimetic. FASEB J. 2011 Mar;25(3):960-7.
REF 40 Cyclin-dependent kinases regulate epigenetic gene silencing through phosphorylation of EZH2. Nat Cell Biol. 2010 Nov;12(11):1108-14.
REF 41 Phosphorylation of the PRC2 component Ezh2 is cell cycle-regulated and up-regulates its binding to ncRNA. Genes Dev. 2010 Dec 1;24(23):2615-20.
REF 42 PCAF-primed EZH2 acetylation regulates its stability and promotes lung adenocarcinoma progression. Nucleic Acids Res. 2015 Apr 20;43(7):3591-604.
REF 43 Downregulation of the cdc2/cyclin B protein kinase activity by binding of p53 to p34(cdc2). Biochem Biophys Res Commun. 2001 May 4;283(2):507-12.
REF 44 The C-terminal regulatory domain of p53 contains a functional docking site for cyclin A. J Mol Biol. 2000 Jul 14;300(3):503-18.
REF 45 Sp1 phosphorylation by cyclin-dependent kinase 1/cyclin B1 represses its DNA-binding activity during mitosis in cancer cells. Oncogene. 2012 Nov 22;31(47):4946-59.
REF 46 Pin1-mediated Sp1 phosphorylation by CDK1 increases Sp1 stability and decreases its DNA-binding activity during mitosis. Nucleic Acids Res. 2014 Dec 16;42(22):13573-87.
REF 47 The TIP60 Complex Is a Conserved Coactivator of HIF1A. Cell Rep. 2016 Jun 28;16(1):37-47.
REF 48 Cyclin-dependent kinases regulate lysosomal degradation of hypoxia-inducible factor 1 to promote cell-cycle progression. Proc Natl Acad Sci U S A. 2014 Aug 12;111(32):E3325-34.
REF 49 Cdc20 and Cks direct the spindle checkpoint-independent destruction of cyclin A. Mol Cell. 2008 May 9;30(3):290-302.
REF 50 Ectopic expression of cdc2/cdc28 kinase subunit Homo sapiens 1 uncouples cyclin B metabolism from the mitotic spindle cell cycle checkpoint. Mol Cell Biol. 1998 Nov;18(11):6224-37.
REF 51 A census of human soluble protein complexes. Cell. 2012 Aug 31;150(5):1068-81.
REF 52 A proteome-scale map of the human interactome network. Cell. 2014 Nov 20;159(5):1212-1226.
REF 53 APC/C(Cdc20) controls the ubiquitin-mediated degradation of p21 in prometaphase. Mol Cell. 2007 Aug 3;27(3):462-73.
REF 54 The GADD45 inhibition of Cdc2 kinase correlates with GADD45-mediated growth suppression. J Biol Chem. 2000 Jun 2;275(22):16602-8.
REF 55 S10 phosphorylation of p27 mediates atRA induced growth arrest in ovarian carcinoma cell lines. J Cell Physiol. 2008 Nov;217(2):558-68.

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