Target Interacting Protein Information

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Target General Information
Target Interacting Proteins
References
Target General Information  Top
Target ID T20953 Target Info
Target Name BUB1 mitotic checkpoint serine/threonine kinase (BUB1)
Synonyms hBUB1; Mitotic checkpoint serine/threonine-protein kinase BUB1; BUB1L; BUB1A
Target Type Patented-recorded Target
Gene Name BUB1
Biochemical Class Kinase
UniProt ID
BUB1_HUMAN 
Target Interacting Proteins  Top
Beta-transducin repeats [+] 2 Beta-transducin repeats +
Interacting Protein Name Mitotic checkpoint protein BUB3 (BUB3) Interacting Protein Interacting Protein Info
UniProt ID
BUB3_HUMAN 
Synonyms BUB3L; hBUB3
Evidence Score (E-score) 6 Experimental Evidences +
1 Affinity Capture-MS [1]
2 Affinity Capture-MS [2]
3 Affinity Capture-MS [3]
4 Yeast two hybrid [4]
5 Yeast two hybrid [5]
6 Yeast two hybrid [6]
Interacting Protein Name Cell division cycle protein 20 homolog (CDC20) Interacting Protein Interacting Protein Info
UniProt ID
CDC20_HUMAN 
Synonyms CDC20A; bA276H19.3; p55CDC
Evidence Score (E-score) 5 Experimental Evidences +
1 Affinity Capture-MS [7]
2 Affinity Capture-MS [8]
3 Affinity Capture-MS [2]
4 Biochemical Activity [9]
5 Protein-peptide [10]
Kinases [+] 1 Kinases +
Interacting Protein Name Mitotic checkpoint serine/threonine-protein kinase BUB1 beta (BUB1B) Interacting Protein Interacting Protein Info
UniProt ID
BUB1B_HUMAN 
Synonyms BUB1beta; BUBR1; Bub1A; MAD3L; MVA1; SSK1; hBUBR1
Evidence Score (E-score) 6 Experimental Evidences +
1 Affinity Capture-MS [11]
2 Affinity Capture-MS [7]
3 Affinity Capture-MS [8]
4 Affinity Capture-MS [3]
5 Co-localization [12]
6 Yeast two hybrid [4]
Membrane-bounded channels [+] 1 Membrane-bounded channels +
Interacting Protein Name mRNA export factor (RAE1) Interacting Protein Interacting Protein Info
UniProt ID
RAE1L_HUMAN 
Synonyms MIG14; MRNP41; Mnrp41; dJ481F12.3; dJ800J21.1
Evidence Score (E-score) 3 Experimental Evidences +
1 Affinity Capture-MS [7]
2 Affinity Capture-MS [8]
3 Affinity Capture-Western [13]
Other proteins [+] 3 Other proteins +
Interacting Protein Name Kinetochore scaffold 1 (KNL1) Interacting Protein Interacting Protein Info
UniProt ID
KNL1_HUMAN 
Synonyms AF15Q14; CT29; D40; KNL1; PPP1R55; Spc7; hKNL.1; hSpc105
Evidence Score (E-score) 7 Experimental Evidences +
1 Affinity Capture-MS [11]
2 Affinity Capture-MS [7]
3 Affinity Capture-MS [8]
4 Affinity Capture-MS [2]
5 Affinity Capture-MS [3]
6 Affinity Capture-Western [14]
7 Yeast two hybrid [15]
Interacting Protein Name Adenomatous polyposis coli protein (APC) Interacting Protein Interacting Protein Info
UniProt ID
APC_HUMAN 
Synonyms BTPS2; DP2; DP2.5; DP3; GS; PPP1R46
Evidence Score (E-score) 3 Experimental Evidences +
1 Affinity Capture-Western [16]
2 Biochemical Activity [16]
3 Co-localization [17]
Interacting Protein Name Kinetochore-associated protein NSL1 homolog (NSL1) Interacting Protein Interacting Protein Info
UniProt ID
NSL1_HUMAN 
Synonyms C1orf48; MIS14
Evidence Score (E-score) 2 Experimental Evidences +
1 Affinity Capture-MS [11]
2 Co-localization [18]
References  Top
REF 1 Phosphorylation- and polo-box-dependent binding of Plk1 to Bub1 is required for the kinetochore localization of Plk1. Mol Biol Cell. 2006 Aug;17(8):3705-16.
REF 2 A human interactome in three quantitative dimensions organized by stoichiometries and abundances. Cell. 2015 Oct 22;163(3):712-23.
REF 3 Dissecting the roles of human BUB1 in the spindle assembly checkpoint. J Cell Sci. 2015 Aug 15;128(16):2975-82.
REF 4 Defining the molecular basis of BubR1 kinetochore interactions and APC/C-CDC20 inhibition. J Biol Chem. 2010 May 7;285(19):14764-76.
REF 5 A proteome-scale map of the human interactome network. Cell. 2014 Nov 20;159(5):1212-1226.
REF 6 Toward an understanding of the protein interaction network of the human liver. Mol Syst Biol. 2011 Oct 11;7:536.
REF 7 Architecture of the human interactome defines protein communities and disease networks. Nature. 2017 May 25;545(7655):505-509.
REF 8 The BioPlex Network: A Systematic Exploration of the Human Interactome. Cell. 2015 Jul 16;162(2):425-440.
REF 9 The Bub1-Plk1 kinase complex promotes spindle checkpoint signalling through Cdc20 phosphorylation. Nat Commun. 2016 Feb 25;7:10818.
REF 10 The ABBA motif binds APC/C activators and is shared by APC/C substrates and regulators. Dev Cell. 2015 Feb 9;32(3):358-372.
REF 11 Systematic analysis of human protein complexes identifies chromosome segregation proteins. Science. 2010 Apr 30;328(5978):593-9.
REF 12 The hBUB1 and hBUBR1 kinases sequentially assemble onto kinetochores during prophase with hBUBR1 concentrating at the kinetochore plates in mitosis. Chromosoma. 1998 Dec;107(6-7):386-96.
REF 13 The mitotic checkpoint protein hBUB3 and the mRNA export factor hRAE1 interact with GLE2p-binding sequence (GLEBS)-containing proteins. J Biol Chem. 2001 Jul 13;276(28):26559-67.
REF 14 MPS1/Mph1 phosphorylates the kinetochore protein KNL1/Spc7 to recruit SAC components. Nat Cell Biol. 2012 Jun 3;14(7):746-52.
REF 15 The crystal structure of the N-terminal region of BUB1 provides insight into the mechanism of BUB1 recruitment to kinetochores. Structure. 2009 Jan 14;17(1):105-16.
REF 16 A role for the Adenomatous Polyposis Coli protein in chromosome segregation. Nat Cell Biol. 2001 Apr;3(4):429-32.
REF 17 Using an in situ proximity ligation assay to systematically profile endogenous protein-protein interactions in a pathway network. J Proteome Res. 2014 Dec 5;13(12):5339-46.
REF 18 Inner centromere formation requires hMis14, a trident kinetochore protein that specifically recruits HP1 to human chromosomes. J Cell Biol. 2010 Mar 22;188(6):791-807.

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