Target Interacting Protein Information

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Target General Information
Target Interacting Proteins
References
Target General Information  Top
Target ID T04831 Target Info
Target Name Tyrosine-protein kinase EIF2AK2 (p68)
Synonyms p68 kinase; Proteinkinase RNA-activated; Interferon-inducible RNA-dependent protein kinase p68; Interferon-induced, double-stranded RNA-activated protein kinase p68
Target Type Patented-recorded Target
Gene Name EIF2AK2
Biochemical Class Kinase
UniProt ID
E2AK2_HUMAN 
Target Interacting Proteins  Top
Acid anhydride hydrolases [+] 2 Acid anhydride hydrolases +
Interacting Protein Name ATP-dependent RNA helicase A (DHX9) Interacting Protein Interacting Protein Info
UniProt ID
DHX9_HUMAN 
Synonyms DDX9; LKP; NDH2; NDHII; RHA
Evidence Score (E-score) 5 Experimental Evidences +
1 Affinity Capture-MS [1]
2 Affinity Capture-MS [2]
3 Affinity Capture-Western [1]
4 Biochemical Activity [1]
5 Reconstituted Complex [2]
Interacting Protein Name ATP-dependent RNA helicase DHX30 (DHX30) Interacting Protein Interacting Protein Info
UniProt ID
DHX30_HUMAN 
Synonyms DDX30; RETCOR
Evidence Score (E-score) 3 Experimental Evidences +
1 Affinity Capture-MS [3]
2 Affinity Capture-MS [2]
3 Reconstituted Complex [2]
Eukaryotic initiation factors [+] 1 Eukaryotic initiation factors +
Interacting Protein Name Eukaryotic translation initiation factor 2 subunit 1 (EIF2S1) Interacting Protein Interacting Protein Info
UniProt ID
IF2A_HUMAN 
Synonyms EIF.2; EIF.2A; EIF.2alpha; EIF2; EIF2A
Evidence Score (E-score) 5 Experimental Evidences +
1 Affinity Capture-Western [4]
2 Biochemical Activity [5]
3 Biochemical Activity [6]
4 Reconstituted Complex [7]
5 Reconstituted Complex [6]
Heat shock proteins [+] 1 Heat shock proteins +
Interacting Target Name Heat shock protein 90 alpha (HSP90A) Successful Target Interacting Protein Info
UniProt ID
HS90A_HUMAN 
Synonyms Renal carcinoma antigen NY-REN-38; LPS-associated protein 2; Lipopolysaccharide-associated protein 2; LAP-2; HSPCA; HSPC1; HSP90A; HSP86; HSP 86; Heat shock protein HSP 90-alpha; Heat shock 86 kDa
Evidence Score (E-score) 2 Experimental Evidences +
1 Affinity Capture-Luminescence [8]
2 Affinity Capture-Western [9]
Pore-forming toxins [+] 1 Pore-forming toxins +
Interacting Target Name Cellular tumor antigen p53 (TP53) Clinical trial Target Interacting Protein Info
UniProt ID
P53_HUMAN 
Synonyms Tumor suppressor p53; Phosphoprotein p53; P53; Antigen NY-CO-13
Evidence Score (E-score) 4 Experimental Evidences +
1 Affinity Capture-Western [10]
2 Affinity Capture-Western [11]
3 Biochemical Activity [10]
4 Reconstituted Complex [10]
Transcription regulators [+] 1 Transcription regulators +
Interacting Target Name Signal transducer and activator of transcription 1 (STAT1) Patented-recorded Target Interacting Protein Info
UniProt ID
STAT1_HUMAN 
Synonyms Transcription factor ISGF-3 components p91/p84; STAT-1; Signal transducers and activators of transcription factor; Signal transducer and activator of transcription 1-alpha/beta; P91
Evidence Score (E-score) 2 Experimental Evidences +
1 Affinity Capture-Western [12]
2 Reconstituted Complex [13]
Other proteins [+] 4 Other proteins +
Interacting Protein Name Interleukin enhancer-binding factor 3 (ILF3) Interacting Protein Interacting Protein Info
UniProt ID
ILF3_HUMAN 
Synonyms CBTF; DRBF; DRBP76; MMP4; MPHOSPH4; MPP4; NF.AT.90; NF110; NF110b; NF90; NF90a; NF90b; NFAR; NFAR.1; NFAR2; TCP110; TCP80
Evidence Score (E-score) 11 Experimental Evidences +
1 Affinity Capture-Western [14]
2 Affinity Capture-Western [15]
3 Biochemical Activity [14]
4 Biochemical Activity [15]
5 Biochemical Activity [16]
6 Biochemical Activity [17]
7 Reconstituted Complex [14]
8 Reconstituted Complex [15]
9 Reconstituted Complex [17]
10 Yeast two hybrid [14]
11 Yeast two hybrid [17]
Interacting Target Name Nucleophosmin (NPM1) Clinical trial Target Interacting Protein Info
UniProt ID
NPM_HUMAN 
Synonyms Numatrin; Nucleolar protein NO38; Nucleolar phosphoprotein B23; NPM
Evidence Score (E-score) 5 Experimental Evidences +
1 Affinity Capture-MS [6]
2 Affinity Capture-Western [18]
3 Affinity Capture-Western [6]
4 Biochemical Activity [6]
5 Reconstituted Complex [6]
Interacting Protein Name RISC-loading complex subunit TARBP2 (TARBP2) Interacting Protein Interacting Protein Info
UniProt ID
TRBP2_HUMAN 
Synonyms LOQS; TRBP; TRBP1; TRBP2
Evidence Score (E-score) 5 Experimental Evidences +
1 Affinity Capture-MS [2]
2 Far Western [19]
3 Reconstituted Complex [2]
4 Yeast two hybrid [19]
5 Yeast two hybrid [20]
Interacting Protein Name PKR-associated protein X (PRKRA) Interacting Protein Interacting Protein Info
UniProt ID
PRKRA_HUMAN 
Synonyms DYT16; PACT; RAX
Evidence Score (E-score) 4 Experimental Evidences +
1 Affinity Capture-MS [3]
2 Affinity Capture-MS [2]
3 Reconstituted Complex [21]
4 Yeast two hybrid [22]
References  Top
REF 1 An antiviral response directed by PKR phosphorylation of the RNA helicase A. PLoS Pathog. 2009 Feb;5(2):e1000311.
REF 2 Mapping a dynamic innate immunity protein interaction network regulating type I interferon production. Immunity. 2011 Sep 23;35(3):426-40.
REF 3 Interlaboratory reproducibility of large-scale human protein-complex analysis by standardized AP-MS. Nat Methods. 2013 Apr;10(4):307-14.
REF 4 Interferons induce tyrosine phosphorylation of the eIF2alpha kinase PKR through activation of Jak1 and Tyk2. EMBO Rep. 2007 Mar;8(3):265-70.
REF 5 Requirement for kinase-induced conformational change in eukaryotic initiation factor 2alpha (eIF2alpha) restricts phosphorylation of Ser51. Proc Natl Acad Sci U S A. 2011 Mar 15;108(11):4316-21.
REF 6 Nucleophosmin interacts with and inhibits the catalytic function of eukaryotic initiation factor 2 kinase PKR. J Biol Chem. 2003 Oct 24;278(43):41709-17.
REF 7 Distal recognition sites in substrates are required for efficient phosphorylation by the cAMP-dependent protein kinase. Genetics. 2009 Jun;182(2):529-39.
REF 8 Quantitative analysis of HSP90-client interactions reveals principles of substrate recognition. Cell. 2012 Aug 31;150(5):987-1001.
REF 9 The Hsp90 chaperone complex is both a facilitator and a repressor of the dsRNA-dependent kinase PKR. EMBO J. 2001 Jul 16;20(14):3771-80.
REF 10 The double-stranded RNA activated protein kinase PKR physically associates with the tumor suppressor p53 protein and phosphorylates human p53 on serine 392 in vitro. Oncogene. 1999 Apr 29;18(17):2690-702.
REF 11 Evidence of molecular links between PKR and mTOR signalling pathways in Abeta neurotoxicity: role of p53, Redd1 and TSC2. Neurobiol Dis. 2009 Oct;36(1):151-61.
REF 12 Physical association between STAT1 and the interferon-inducible protein kinase PKR and implications for interferon and double-stranded RNA signaling pathways. EMBO J. 1997 Mar 17;16(6):1291-304.
REF 13 Enhanced antiviral and antiproliferative properties of a STAT1 mutant unable to interact with the protein kinase PKR. J Biol Chem. 2001 Apr 27;276(17):13727-37.
REF 14 Characterization of two evolutionarily conserved, alternatively spliced nuclear phosphoproteins, NFAR-1 and -2, that function in mRNA processing and interact with the double-stranded RNA-dependent protein kinase, PKR. J Biol Chem. 2001 Aug 24;276(34):32300-12.
REF 15 Nuclear factor 90 is a substrate and regulator of the eukaryotic initiation factor 2 kinase double-stranded RNA-activated protein kinase. J Biol Chem. 2001 Aug 31;276(35):32522-30.
REF 16 Nuclear factor-90 of activated T-cells: A double-stranded RNA-binding protein and substrate for the double-stranded RNA-dependent protein kinase, PKR. Biochemistry. 1999 May 11;38(19):6361-8.
REF 17 DRBP76, a double-stranded RNA-binding nuclear protein, is phosphorylated by the interferon-induced protein kinase, PKR. J Biol Chem. 1999 Jul 16;274(29):20432-7.
REF 18 Antiviral action of the tumor suppressor ARF. EMBO J. 2006 Sep 20;25(18):4284-92.
REF 19 Double-stranded-RNA-dependent protein kinase and TAR RNA-binding protein form homo- and heterodimers in vivo. Proc Natl Acad Sci U S A. 1995 Oct 10;92(21):9445-9.
REF 20 Two dimerization domains in the trans-activation response RNA-binding protein (TRBP) individually reverse the protein kinase R inhibition of HIV-1 long terminal repeat expression. J Biol Chem. 2001 Sep 7;276(36):33899-905.
REF 21 The C-terminal, third conserved motif of the protein activator PACT plays an essential role in the activation of double-stranded-RNA-dependent protein kinase (PKR). Biochem J. 2002 Aug 15;366(Pt 1):175-86.
REF 22 PACT, a protein activator of the interferon-induced protein kinase, PKR. EMBO J. 1998 Aug 3;17(15):4379-90.

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