Target Binding Site Detail
| Target General Information | Top | ||||
|---|---|---|---|---|---|
| Target ID | T33367 | Target Info | |||
| Target Name | Cytoplasmic aspartate aminotransferase (GOT1) | ||||
| Synonyms | Glutamate oxaloacetate transaminase-1; GOT1 | ||||
| Target Type | Literature-reported Target | ||||
| Gene Name | GOT1 | ||||
| Biochemical Class | Transaminase | ||||
| UniProt ID | |||||
| Ligand General Information | Top | ||||
|---|---|---|---|---|---|
| Ligand Name | Pyridoxal phosphate | Ligand Info | |||
| Canonical SMILES | CC1=NC=C(C(=C1O)C=O)COP(=O)(O)O | ||||
| InChI | 1S/C8H10NO6P/c1-5-8(11)7(3-10)6(2-9-5)4-15-16(12,13)14/h2-3,11H,4H2,1H3,(H2,12,13,14) | ||||
| InChIKey | NGVDGCNFYWLIFO-UHFFFAOYSA-N | ||||
| PubChem Compound ID | 1051 | ||||
| Drug Binding Sites of Target | Top | |||||
|---|---|---|---|---|---|---|
| PDB ID: 6DND Crystal structure of wild-type (WT) human Glutamate oxaloacetate transaminase 1 (GOT1) | ||||||
| Method | X-ray diffraction | Resolution | 2.10 Å | Mutation | No | [1] |
| PDB Sequence |
PPSVFAEVPQ
12 AQPVLVFKLT22 ADFREDPDPR32 KVNLGVGAYR42 TDDCHPWVLP52 VVKKVEQKIA 62 NDNSLNHEYL72 PILGLAEFRS82 CASRLALGDD92 SPALKEKRVG102 GVQSLGGTGA 112 LRIGADFLAR122 WYNGTNNKNT132 PVYVSSPTWE142 NHNAVFSAAG152 FKDIRSYRYW 162 DAEKRGLDLQ172 GFLNDLENAP182 EFSIVVLHAC192 AHNPTGIDPT202 PEQWKQIASV 212 MKHRFLFPFF222 DSAYQGFASG232 NLERDAWAIR242 YFVSEGFEFF252 CAQSFSKNFG 262 LYNERVGNLT272 VVGKEPESIL282 QVLSQMEKIV292 RITWSNPPAQ302 GARIVASTLS 312 NPELFEEWTG322 NVKTMADRIL332 TMRSELRARL342 EALKTPGTWN352 HITDQIGMFS 362 FTGLNPKQVE372 YLVNEKHIYL382 LPSGRINVSG392 LTTKNLDYVA402 TSIHEAVTK |
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| PDB ID: 6DNA Crystal structure of T110A mutant human Glutamate oxaloacetate transaminase 1 (GOT1) | ||||||
| Method | X-ray diffraction | Resolution | 3.00 Å | Mutation | Yes | [1] |
| PDB Sequence |
VLVFKLTADF
25 REDPDPRKVN35 LGVGAYRTDD45 CHPWVLPVVK55 KVEQKIANDN65 SLNHEYLPIL 75 GLAEFRSCAS85 RLALGDDSPA95 LKEKRVGGVQ105 SLGGAGALRI115 GADFLARWYN 125 GTNNKNTPVY135 VSSPTWENHN145 AVFSAAGFKD155 IRSYRYWDAE165 KRGLDLQGFL 175 NDLENAPEFS185 IVVLHACAHN195 PTGIDPTPEQ205 WKQIASVMKH215 RFLFPFFDSA 225 YQGFSGNLER236 DAWAIRYFVS246 EGFEFFCAQS256 FSKNFGLYNE266 RVGNLTVVGK 276 EPESILQVLS286 QMEKIVRITW296 SNPPAQGARI306 VASTLSNPEL316 FEEWTGNVKT 326 MADRILTMRS336 ELRARLEALK346 TPGTWNHITD356 QIGMFSFTGL366 NPKQVEYLHI 380 YLLPSGRINV390 SGLTTKNLDY400 VATSIHEAVT410
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| PDB ID: 3II0 Crystal structure of human Glutamate oxaloacetate transaminase 1 (GOT1) | ||||||
| Method | X-ray diffraction | Resolution | 2.05 Å | Mutation | No | [2] |
| PDB Sequence |
PVLVFKLTAD
24 FREDPDPRKV34 NLGVGAYRTD44 DCHPWVLPVV54 KKVEQKIAND64 NSLNHEYLPI 74 LGLAEFRSCA84 SRLALGDDSP94 ALKEKRVGGV104 QSLGGTGALR114 IGADFLARWY 124 NGTNNKNTPV134 YVSSPTWENH144 NAVFSAAGFK154 DIRSYRYWDA164 EKRGLDLQGF 174 LNDLENAPEF184 SIVVLHACAH194 NPTGIDPTPE204 QWKQIASVMK214 HRFLFPFFDS 224 AYQGFASGNL234 ERDAWAIRYF244 VSEGFEFFCA254 QSFSKNFGLY264 NERVGNLTVV 274 GKEPESILQV284 LSQMEKIVRI294 TWSNPPAQGA304 RIVASTLSNP314 ELFEEWTGNV 324 KTMADRILTM334 RSELRARLEA344 LKTPGTWNHI354 TDQIGMFSFT364 GLNPKQVEYL 374 VNEKHIYLLP384 SGRINVSGLT394 TKNLDYVATS404 IHEAVTKIAE414 NLYFQ |
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| References | Top | ||||
|---|---|---|---|---|---|
| REF 1 | Biochemical Characterization and Structure-Based Mutational Analysis Provide Insight into the Binding and Mechanism of Action of Novel Aspartate Aminotransferase Inhibitors. Biochemistry. 2018 Nov 27;57(47):6604-6614. | ||||
| REF 2 | Crystal structure of human Glutamate oxaloacetate transaminase 1 (GOT1) | ||||
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