Target Interacting Protein Information

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Target General Information
Target Interacting Proteins
References
Target General Information  Top
Target ID T99089 Target Info
Target Name Serine/threonine-protein kinase B-raf (BRAF)
Synonyms V-Raf murine sarcoma viral oncogene homolog B1; RAFB1; Proto-oncogene B-Raf; P94; BRAF1; BRAF(V599E); BRAF serine/threonine kinase; B-raf protein; B-Raf
Target Type Successful Target
Gene Name BRAF
Biochemical Class Kinase
UniProt ID
BRAF_HUMAN 
Target Interacting Proteins  Top
Acid anhydride hydrolases [+] 4 Acid anhydride hydrolases +
Interacting Target Name GTPase HRas (HRAS) Literature-reported Target Interacting Protein Info
UniProt ID
RASH_HUMAN 
Synonyms Transforming protein p21; p21ras; H-Ras-1; Ha-Ras; HaRas; GTPase HRas, Nterminally processed; GTPase HRas; c-H-ras; cHras
Evidence Score (E-score) 4 Experimental Evidences +
1 Affinity Capture-MS [1]
2 Fluorescence Resonance Energy Transfer [2]
3 Reconstituted Complex [3]
4 Yeast two hybrid [4]
Interacting Target Name GTPase NRas (NRAS) Clinical trial Target Interacting Protein Info
UniProt ID
RASN_HUMAN 
Synonyms Transforming protein N-Ras; HRAS1
Evidence Score (E-score) 2 Experimental Evidences +
1 Affinity Capture-Western [5]
2 Yeast two hybrid [6]
Interacting Target Name GTPase KRas (KRAS) Clinical trial Target Interacting Protein Info
UniProt ID
RASK_HUMAN 
Synonyms K-Ras 2; Ki-Ras; c-K-ras; c-Ki-ras
Evidence Score (E-score) 2 Experimental Evidences +
1 Affinity Capture-MS [7]
2 Fluorescence Resonance Energy Transfer [2]
Interacting Target Name Endoplasmic reticulum chaperone BiP (HSPA5) Clinical trial Target Interacting Protein Info
UniProt ID
BIP_HUMAN 
Synonyms Immunoglobulin heavy chain-binding protein; Immunoglobulin heavy chainbinding protein; HSP70 family protein 5; Heat shock protein family A member 5; Heat shock protein 70 family protein 5; Heat shock 70 kDa protein 5; GRP-78; GRP78; Endoplasmic reticulum lumenal Ca(2+)binding protein grp78; BiP; Binding-immunoglobulin protein; 78 kDa glucose-regulated protein
Evidence Score (E-score) 1 Experimental Evidences +
1 Affinity Capture-MS [8]
Auxiliary transporters [+] 1 Auxiliary transporters +
Interacting Protein Name 14-3-3 protein zeta/delta (YWHAZ) Interacting Protein Interacting Protein Info
UniProt ID
1433Z_HUMAN 
Synonyms 14.3.3.zeta; HEL.S.3; HEL4; KCIP.1; YWHAD
Evidence Score (E-score) 2 Experimental Evidences +
1 Affinity Capture-MS [8]
2 Fluorescence Resonance Energy Transfer [2]
Cell division cycle proteins [+] 1 Cell division cycle proteins +
Interacting Target Name Hsp90 co-chaperone Cdc37 (CDC37) Literature-reported Target Interacting Protein Info
UniProt ID
CDC37_HUMAN 
Synonyms p50Cdc37; Hsp90 co-chaperone Cdc37, N-terminally processed; Hsp90 chaperone protein kinase-targeting subunit; CDC37A
Evidence Score (E-score) 1 Experimental Evidences +
1 Affinity Capture-MS [8]
Heat shock proteins [+] 4 Heat shock proteins +
Interacting Target Name Mortalin (HSPA9) Clinical trial Target Interacting Protein Info
UniProt ID
GRP75_HUMAN 
Synonyms Stress-70 protein, mitochondrial; Peptide-binding protein 74; PBP74; mt-HSP70; MOT; HSPA9B; Heat shock 70 kDa protein 9; GRP-75; GRP75; 75 kDa glucose-regulated protein
Evidence Score (E-score) 1 Experimental Evidences +
1 Affinity Capture-MS [8]
Interacting Target Name Heat shock protein 90 beta (HSP90B) Clinical trial Target Interacting Protein Info
UniProt ID
HS90B_HUMAN 
Synonyms HSP84; HSP 84; Heat shock 84 kDa
Evidence Score (E-score) 1 Experimental Evidences +
1 Affinity Capture-MS [8]
Interacting Target Name Lipopolysaccharide-associated protein 1 (HSPA8) Literature-reported Target Interacting Protein Info
UniProt ID
HSP7C_HUMAN 
Synonyms LPS-associated protein 1; LAP-1; HSPA10; HSP73; HSC70; Heat shock protein 73; Heat shock cognate 71 kDa protein; Heat shock 70 kDa protein 8
Evidence Score (E-score) 1 Experimental Evidences +
1 Affinity Capture-MS [8]
Interacting Protein Name Heat shock 70 kDa protein 1A (HSPA1A) Interacting Protein Interacting Protein Info
UniProt ID
HS71A_HUMAN 
Synonyms HEL.S.103; HSP70.1; HSP70.1A; HSP70I; HSP72; HSPA1
Evidence Score (E-score) 1 Experimental Evidences +
1 Affinity Capture-MS [8]
Kinases [+] 5 Kinases +
Interacting Target Name RAC-alpha serine/threonine-protein kinase (AKT1) Successful Target Interacting Protein Info
UniProt ID
AKT1_HUMAN 
Synonyms RAC-PK-alpha; RAC; Proto-oncogene c-Akt; Protein kinase B alpha; Protein kinase B; PKB alpha; PKB
Evidence Score (E-score) 4 Experimental Evidences +
1 Affinity Capture-Western [9]
2 Biochemical Activity [9]
3 Co-localization [10]
4 Fluorescence Resonance Energy Transfer [2]
Interacting Target Name ERK activator kinase 1 (MEK1) Clinical trial Target Interacting Protein Info
UniProt ID
MP2K1_HUMAN 
Synonyms PRKMK1; MKK1; Mitogen-activated protein kinase kinase 1; MEK 1; MAPKK 1; MAPK/ERKkinase 1; MAPK/ERK kinase 1; MAP kinase kinase 1; Dual specificity mitogen-activated protein kinase kinase 1
Evidence Score (E-score) 4 Experimental Evidences +
1 Affinity Capture-MS [8]
2 Affinity Capture-Western [11]
3 Biochemical Activity [12]
4 Reconstituted Complex [13]
Interacting Target Name Proto-oncogene c-RAF (c-RAF) Clinical trial Target Interacting Protein Info
UniProt ID
RAF1_HUMAN 
Synonyms Raf-1; RAF proto-oncogene serine/threonine-protein kinase; RAF; cRaf
Evidence Score (E-score) 2 Experimental Evidences +
1 Affinity Capture-MS [14]
2 Affinity Capture-Western [15]
Interacting Protein Name Proto-oncogene A-Raf (ARAF ) Interacting Protein Interacting Protein Info
UniProt ID
ARAF_HUMAN 
Synonyms Serine/threonine-protein kinase A-Raf; Proto-oncogene Pks; Proto-oncogene A-Raf-1; Proto-oncogene A-Raf; PKS2; PKS; ARAF1
Evidence Score (E-score) 2 Experimental Evidences +
1 Affinity Capture-MS [8]
2 Fluorescence Resonance Energy Transfer [2]
Interacting Target Name ERK activator kinase 2 (MEK2) Clinical trial Target Interacting Protein Info
UniProt ID
MP2K2_HUMAN 
Synonyms PRKMK2; MKK2; MEK 2; MAPKK 2; MAPK/ERK kinase 2; MAP kinase kinase 2; Dual specificity mitogen-activated protein kinase kinase 2; Dual specificity mitogenactivated protein kinase kinase 2
Evidence Score (E-score) 1 Experimental Evidences +
1 Affinity Capture-MS [8]
Proteins 14-3-3 [+] 6 Proteins 14-3-3 +
Interacting Protein Name 14-3-3 protein beta/alpha (YWHAB) Interacting Protein Interacting Protein Info
UniProt ID
1433B_HUMAN 
Synonyms GW128; HEL.S.1; HS1; KCIP.1; YWHAA
Evidence Score (E-score) 2 Experimental Evidences +
1 Affinity Capture-MS [1]
2 Affinity Capture-Western [16]
Interacting Protein Name 14-3-3 protein gamma (YWHAG) Interacting Protein Interacting Protein Info
UniProt ID
1433G_HUMAN 
Synonyms 14.3.3GAMMA; PPP1R170
Evidence Score (E-score) 2 Experimental Evidences +
1 Affinity Capture-MS [8]
2 Fluorescence Resonance Energy Transfer [2]
Interacting Protein Name 14-3-3 protein sigma (SFN) Interacting Protein Interacting Protein Info
UniProt ID
1433S_HUMAN 
Synonyms YWHAS
Evidence Score (E-score) 2 Experimental Evidences +
1 Affinity Capture-MS [1]
2 Fluorescence Resonance Energy Transfer [2]
Interacting Protein Name 14-3-3 protein theta (YWHAQ) Interacting Protein Interacting Protein Info
UniProt ID
1433T_HUMAN 
Synonyms 14.3.3; 1C5; HS1
Evidence Score (E-score) 2 Experimental Evidences +
1 Affinity Capture-MS [8]
2 Affinity Capture-Western [11]
Interacting Target Name AS1 14-3-3 protein eta (YWHAH) Literature-reported Target Interacting Protein Info
UniProt ID
1433F_HUMAN 
Synonyms YWHA1; Protein AS1; 14-3-3 protein eta
Evidence Score (E-score) 1 Experimental Evidences +
1 Affinity Capture-MS [8]
Interacting Protein Name 14-3-3 protein epsilon (YWHAE) Interacting Protein Interacting Protein Info
UniProt ID
1433E_HUMAN 
Synonyms 14.3.3E; HEL2; KCIP.1; MDCR; MDS
Evidence Score (E-score) 1 Experimental Evidences +
1 Affinity Capture-MS [8]
Other proteins [+] 1 Other proteins +
Interacting Protein Name Ras GTPase-activating-like protein IQGAP1 (IQGAP1) Interacting Protein Interacting Protein Info
UniProt ID
IQGA1_HUMAN 
Synonyms HUMORFA01; SAR1; p195
Evidence Score (E-score) 2 Experimental Evidences +
1 Affinity Capture-MS [1]
2 Reconstituted Complex [17]
References  Top
REF 1 Phospho-proteomic analyses of B-Raf protein complexes reveal new regulatory principles. Oncotarget. 2016 May 3;7(18):26628-52.
REF 2 OncoPPi-informed discovery of mitogen-activated protein kinase kinase 3 as a novel binding partner of c-Myc. Oncogene. 2017 Oct 19;36(42):5852-5860.
REF 3 Biochemical analysis of MEK activation in NIH3T3 fibroblasts. Identification of B-Raf and other activators. J Biol Chem. 1995 Mar 31;270(13):7644-55.
REF 4 Interaction of activated Ras with Raf-1 alone may be sufficient for transformation of rat2 cells. Mol Cell Biol. 1997 Jun;17(6):3047-55.
REF 5 Inhibition of Ras/Raf interaction by anti-oncogenic mutants of neurofibromin, the neurofibromatosis type 1 (NF1) gene product, in cell-free systems. J Biol Chem. 1995 Dec 1;270(48):28834-8.
REF 6 Discrimination of amino acids mediating Ras binding from noninteracting residues affecting raf activation by double mutant analysis. J Biol Chem. 1997 Nov 21;272(47):29927-33.
REF 7 Genetic and Proteomic Interrogation of Lower Confidence Candidate Genes Reveals Signaling Networks in -Catenin-Active Cancers. Cell Syst. 2016 Sep 28;3(3):302-316.e4.
REF 8 A novel tandem affinity purification strategy for the efficient isolation and characterisation of native protein complexes. Proteomics. 2007 Dec;7(23):4228-34.
REF 9 Negative regulation of the serine/threonine kinase B-Raf by Akt. J Biol Chem. 2000 Sep 1;275(35):27354-9.
REF 10 Using an in situ proximity ligation assay to systematically profile endogenous protein-protein interactions in a pathway network. J Proteome Res. 2014 Dec 5;13(12):5339-46.
REF 11 IMP modulates KSR1-dependent multivalent complex formation to specify ERK1/2 pathway activation and response thresholds. J Biol Chem. 2008 May 9;283(19):12789-96.
REF 12 Mammalian Ste20-like kinase (Mst2) indirectly supports Raf-1/ERK pathway activity via maintenance of protein phosphatase-2A catalytic subunit levels and consequent suppression of inhibitory Raf-1 phosphorylation. J Biol Chem. 2010 May 14;285(20):15076-87.
REF 13 The crystal structure of BRAF in complex with an organoruthenium inhibitor reveals a mechanism for inhibition of an active form of BRAF kinase. Biochemistry. 2009 Jun 16;48(23):5187-98.
REF 14 Architecture of the human interactome defines protein communities and disease networks. Nature. 2017 May 25;545(7655):505-509.
REF 15 Active Ras induces heterodimerization of cRaf and BRaf. Cancer Res. 2001 May 1;61(9):3595-8.
REF 16 Cell type-specific regulation of B-Raf kinase by cAMP and 14-3-3 proteins. J Biol Chem. 2000 Oct 13;275(41):31921-9.
REF 17 IQGAP1 integrates Ca2+/calmodulin and B-Raf signaling. J Biol Chem. 2008 Aug 22;283(34):22972-82.

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