Target Interacting Protein Information

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Target General Information
Target Interacting Proteins
References
Target General Information  Top
Target ID T84616 Target Info
Target Name Beclin-1 (BECN1)
Synonyms Protein GT197; GT197; Coiledcoil myosinlike BCL2interacting protein; Coiled-coil myosin-like BCL2-interacting protein
Target Type Literature-reported Target
Gene Name BECN1
UniProt ID
BECN1_HUMAN 
Target Interacting Proteins  Top
Acyltransferases [+] 1 Acyltransferases +
Interacting Target Name E3 ubiquitin-protein ligase NEDD4 (NEDD4) Literature-reported Target Interacting Protein Info
UniProt ID
NEDD4_HUMAN 
Synonyms Neural precursor cell expressed developmentally down-regulated protein 4; NEDD4-1; NEDD-4; KIAA0093; HECT-type E3 ubiquitin transferase NEDD4; Cell proliferation-inducing gene 53 protein
Evidence Score (E-score) 5 Experimental Evidences +
1 Affinity Capture-Western [1]
2 Affinity Capture-Western [2]
3 Affinity Capture-Western [3]
4 Reconstituted Complex [3]
5 Yeast two hybrid [3]
Alpha-type channels [+] 1 Alpha-type channels +
Interacting Target Name Apoptosis regulator Bcl-xL (BCL-xL) Clinical trial Target Interacting Protein Info
UniProt ID
B2CL1_HUMAN 
Synonyms Bcl-XL; BCLX; Bcl-2-like protein 1; Bcl2like protein 1; Bcl2-L-1; Bcl2L1; BCL2L; Apoptosis regulator Bcl-X
Evidence Score (E-score) 8 Experimental Evidences +
1 Affinity Capture-Western [4]
2 Affinity Capture-Western [5]
3 Affinity Capture-Western [6]
4 Affinity Capture-Western [7]
5 Affinity Capture-Western [8]
6 Affinity Capture-Western [9]
7 Reconstituted Complex [6]
8 Yeast two hybrid [10]
Auxiliary transporters [+] 1 Auxiliary transporters +
Interacting Target Name Apoptosis regulator Bcl-2 (BCL-2) Successful Target Interacting Protein Info
UniProt ID
BCL2_HUMAN 
Synonyms Bcl-2; Apoptosis regulator Bcl-2
Evidence Score (E-score) 19 Experimental Evidences +
1 Affinity Capture-Western [11]
2 Affinity Capture-Western [12]
3 Affinity Capture-Western [13]
4 Affinity Capture-Western [14]
5 Affinity Capture-Western [15]
6 Affinity Capture-Western [16]
7 Affinity Capture-Western [17]
8 Affinity Capture-Western [18]
9 Affinity Capture-Western [19]
10 Affinity Capture-Western [20]
11 Affinity Capture-Western [21]
12 Affinity Capture-Western [22]
13 Affinity Capture-Western [23]
14 Affinity Capture-Western [2]
15 Affinity Capture-Western [24]
16 Co-purification [6]
17 Fluorescence Resonance Energy Transfer [17]
18 Reconstituted Complex [6]
19 Yeast two hybrid [10]
Kinases [+] 3 Kinases +
Interacting Protein Name Phosphatidylinositol 3-kinase catalytic subunit type 3 (PIK3C3) Interacting Protein Interacting Protein Info
UniProt ID
PK3C3_HUMAN 
Synonyms VPS34; hVps34
Evidence Score (E-score) 20 Experimental Evidences +
1 Affinity Capture-MS [25]
2 Affinity Capture-MS [26]
3 Affinity Capture-MS [27]
4 Affinity Capture-MS [18]
5 Affinity Capture-Western [16]
6 Affinity Capture-Western [28]
7 Affinity Capture-Western [17]
8 Affinity Capture-Western [18]
9 Affinity Capture-Western [29]
10 Affinity Capture-Western [30]
11 Affinity Capture-Western [31]
12 Affinity Capture-Western [32]
13 Affinity Capture-Western [33]
14 Affinity Capture-Western [34]
15 Affinity Capture-Western [2]
16 Affinity Capture-Western [24]
17 Affinity Capture-Western [9]
18 Affinity Capture-Western [3]
19 Affinity Capture-Western [35]
20 Affinity Capture-Western [36]
Interacting Protein Name Phosphoinositide 3-kinase regulatory subunit 4 (PIK3R4) Interacting Protein Interacting Protein Info
UniProt ID
PI3R4_HUMAN 
Synonyms VPS15; p150
Evidence Score (E-score) 7 Experimental Evidences +
1 Affinity Capture-MS [25]
2 Affinity Capture-MS [26]
3 Affinity Capture-MS [27]
4 Affinity Capture-MS [18]
5 Affinity Capture-Western [17]
6 Affinity Capture-Western [31]
7 Fluorescence Resonance Energy Transfer [17]
Interacting Target Name Serine/threonine-protein kinase ULK1 (ULK1) Literature-reported Target Interacting Protein Info
UniProt ID
ULK1_HUMAN 
Synonyms Unc-51-like kinase 1; KIAA0722; hATG1; Autophagy-related protein 1 homolog; ATG1
Evidence Score (E-score) 2 Experimental Evidences +
1 Affinity Capture-Western [37]
2 Biochemical Activity [38]
Phagophore-form transporters [+] 1 Phagophore-form transporters +
Interacting Protein Name Beclin 1-associated autophagy-related key regulator (ATG14) Interacting Protein Interacting Protein Info
UniProt ID
BAKOR_HUMAN 
Synonyms ATG14L; BARKOR; KIAA0831
Evidence Score (E-score) 17 Experimental Evidences +
1 Affinity Capture-MS [25]
2 Affinity Capture-MS [26]
3 Affinity Capture-MS [27]
4 Affinity Capture-MS [18]
5 Affinity Capture-Western [19]
6 Affinity Capture-Western [17]
7 Affinity Capture-Western [27]
8 Affinity Capture-Western [18]
9 Affinity Capture-Western [29]
10 Affinity Capture-Western [30]
11 Affinity Capture-Western [31]
12 Affinity Capture-Western [32]
13 Affinity Capture-Western [33]
14 Affinity Capture-Western [34]
15 Fluorescence Resonance Energy Transfer [17]
16 Reconstituted Complex [18]
17 Yeast two hybrid [27]
Other proteins [+] 5 Other proteins +
Interacting Protein Name UV radiation resistance-associated gene protein (UVRAG) Interacting Protein Interacting Protein Info
UniProt ID
UVRAG_HUMAN 
Synonyms DHTX; VPS38; p63
Evidence Score (E-score) 11 Experimental Evidences +
1 Affinity Capture-MS [25]
2 Affinity Capture-MS [26]
3 Affinity Capture-MS [27]
4 Affinity Capture-MS [18]
5 Affinity Capture-Western [27]
6 Affinity Capture-Western [18]
7 Affinity Capture-Western [30]
8 Affinity Capture-Western [31]
9 Reconstituted Complex [6]
10 Reconstituted Complex [18]
11 Yeast two hybrid [27]
Interacting Protein Name Activating molecule in BECN1-regulated autophagy protein 1 (AMBRA1) Interacting Protein Interacting Protein Info
UniProt ID
AMRA1_HUMAN 
Synonyms DCAF3; WDR94
Evidence Score (E-score) 9 Experimental Evidences +
1 Affinity Capture-MS [25]
2 Affinity Capture-Western [39]
3 Affinity Capture-Western [28]
4 Affinity Capture-Western [17]
5 Affinity Capture-Western [40]
6 Affinity Capture-Western [41]
7 Affinity Capture-Western [42]
8 Fluorescence Resonance Energy Transfer [17]
9 Reconstituted Complex [28]
Interacting Protein Name Beclin-1 associated RUN domain containing protein (RUBCN) Interacting Protein Interacting Protein Info
UniProt ID
RUBIC_HUMAN 
Synonyms RUBICON; SCAR15
Evidence Score (E-score) 8 Experimental Evidences +
1 Affinity Capture-MS [25]
2 Affinity Capture-MS [26]
3 Affinity Capture-MS [27]
4 Affinity Capture-Western [19]
5 Affinity Capture-Western [27]
6 Affinity Capture-Western [29]
7 Affinity Capture-Western [34]
8 Affinity Capture-Western [43]
Interacting Protein Name WASH complex subunit 1 (WASHC1) Interacting Protein Interacting Protein Info
UniProt ID
WASH1_HUMAN 
Synonyms FAM39E; WASH
Evidence Score (E-score) 3 Experimental Evidences +
1 Affinity Capture-Western [28]
2 Reconstituted Complex [28]
3 Yeast two hybrid [35]
Interacting Protein Name Hamartin (TSC1) Interacting Protein Interacting Protein Info
UniProt ID
TSC1_HUMAN 
Synonyms LAM; TSC
Evidence Score (E-score) 2 Experimental Evidences +
1 Affinity Capture-MS [25]
2 Fluorescence Resonance Energy Transfer [44]
References  Top
REF 1 The E3 ubiquitin ligase NEDD4 enhances killing of membrane-perturbing intracellular bacteria by promoting autophagy. Autophagy. 2017;13(12):2041-2055.
REF 2 Advanced glycation end products (AGE) potentiates cell death in p53 negative cells via upregulaion of NF-kappa B and impairment of autophagy. J Cell Physiol. 2017 Dec;232(12):3598-3610.
REF 3 Nedd4-dependent lysine-11-linked polyubiquitination of the tumour suppressor Beclin 1. Biochem J. 2012 Jan 1;441(1):399-406.
REF 4 Vitamin D3 induces autophagy of human myeloid leukemia cells. J Biol Chem. 2008 Sep 12;283(37):25596-605.
REF 5 ARF induces autophagy by virtue of interaction with Bcl-xl. J Biol Chem. 2009 Jan 30;284(5):2803-10.
REF 6 Bcl-xL and UVRAG cause a monomer-dimer switch in Beclin1. J Biol Chem. 2008 Sep 19;283(38):26274-82.
REF 7 Obatoclax and lapatinib interact to induce toxic autophagy through NOXA. Mol Pharmacol. 2012 Apr;81(4):527-40.
REF 8 DAP-kinase-mediated phosphorylation on the BH3 domain of beclin 1 promotes dissociation of beclin 1 from Bcl-XL and induction of autophagy. EMBO Rep. 2009 Mar;10(3):285-92.
REF 9 Apoptosis blocks Beclin 1-dependent autophagosome synthesis: an effect rescued by Bcl-xL. Cell Death Differ. 2010 Feb;17(2):268-77.
REF 10 Protection against fatal Sindbis virus encephalitis by beclin, a novel Bcl-2-interacting protein. J Virol. 1998 Nov;72(11):8586-96.
REF 11 The VMP1-Beclin 1 interaction regulates autophagy induction. Sci Rep. 2013;3:1055.
REF 12 S100A8-targeting siRNA enhances arsenic trioxide-induced myeloid leukemia cell death by down-regulating autophagy. Int J Mol Med. 2012 Jan;29(1):65-72.
REF 13 Hedgehog signaling pathway regulates autophagy in human hepatocellular carcinoma cells. Hepatology. 2013 Sep;58(3):995-1010.
REF 14 HMGB1 release and redox regulates autophagy and apoptosis in cancer cells. Oncogene. 2010 Sep 23;29(38):5299-310.
REF 15 The GST-BHMT assay reveals a distinct mechanism underlying proteasome inhibition-induced macroautophagy in mammalian cells. Autophagy. 2015;11(5):812-32.
REF 16 Bcl-2 antiapoptotic proteins inhibit Beclin 1-dependent autophagy. Cell. 2005 Sep 23;122(6):927-39.
REF 17 Defective CFTR induces aggresome formation and lung inflammation in cystic fibrosis through ROS-mediated autophagy inhibition. Nat Cell Biol. 2010 Sep;12(9):863-75.
REF 18 Identification of Barkor as a mammalian autophagy-specific factor for Beclin 1 and class III phosphatidylinositol 3-kinase. Proc Natl Acad Sci U S A. 2008 Dec 9;105(49):19211-6.
REF 19 IRGM governs the core autophagy machinery to conduct antimicrobial defense. Mol Cell. 2015 May 7;58(3):507-21.
REF 20 Removal of the BH4 domain from Bcl-2 protein triggers an autophagic process that impairs tumor growth. Neoplasia. 2013 Mar;15(3):315-27.
REF 21 Bcl-2 complexed with Beclin-1 maintains full anti-apoptotic function. Oncogene. 2009 May 28;28(21):2128-41.
REF 22 Omega-3 PUFA ethanolamides DHEA and EPEA induce autophagy through PPAR activation in MCF-7 breast cancer cells. J Cell Physiol. 2013 Jun;228(6):1314-22.
REF 23 The IKK complex contributes to the induction of autophagy. EMBO J. 2010 Feb 3;29(3):619-31.
REF 24 CaMKII-mediated Beclin 1 phosphorylation regulates autophagy that promotes degradation of Id and neuroblastoma cell differentiation. Nat Commun. 2017 Oct 27;8(1):1159.
REF 25 Network organization of the human autophagy system. Nature. 2010 Jul 1;466(7302):68-76.
REF 26 A human interactome in three quantitative dimensions organized by stoichiometries and abundances. Cell. 2015 Oct 22;163(3):712-23.
REF 27 Two Beclin 1-binding proteins, Atg14L and Rubicon, reciprocally regulate autophagy at different stages. Nat Cell Biol. 2009 Apr;11(4):385-96.
REF 28 WASH inhibits autophagy through suppression of Beclin 1 ubiquitination. EMBO J. 2013 Oct 16;32(20):2685-96.
REF 29 Dapper1 promotes autophagy by enhancing the Beclin1-Vps34-Atg14L complex formation. Cell Res. 2014 Aug;24(8):912-24.
REF 30 Beclin 1 forms two distinct phosphatidylinositol 3-kinase complexes with mammalian Atg14 and UVRAG. Mol Biol Cell. 2008 Dec;19(12):5360-72.
REF 31 Role of membrane association and Atg14-dependent phosphorylation in beclin-1-mediated autophagy. Mol Cell Biol. 2013 Sep;33(18):3675-88.
REF 32 The stress-responsive kinases MAPKAPK2/MAPKAPK3 activate starvation-induced autophagy through Beclin 1 phosphorylation. Elife. 2015 Feb 18;4.
REF 33 The BEACH-containing protein WDR81 coordinates p62 and LC3C to promote aggrephagy. J Cell Biol. 2017 May 1;216(5):1301-1320.
REF 34 The Effect of and Mechanism Underlying Autophagy in Hepatocellular Carcinoma Induced by CH12, a Monoclonal Antibody Directed Against Epidermal Growth Factor Receptor Variant III. Cell Physiol Biochem. 2018;46(1):226-237.
REF 35 RNF2 is recruited by WASH to ubiquitinate AMBRA1 leading to downregulation of autophagy. Cell Res. 2014 Aug;24(8):943-58.
REF 36 VMA21 deficiency prevents vacuolar ATPase assembly and causes autophagic vacuolar myopathy. Acta Neuropathol. 2013 Mar;125(3):439-57.
REF 37 RalB and the exocyst mediate the cellular starvation response by direct activation of autophagosome assembly. Cell. 2011 Jan 21;144(2):253-67.
REF 38 TRIMs and Galectins Globally Cooperate and TRIM16 and Galectin-3 Co-direct Autophagy in Endomembrane Damage Homeostasis. Dev Cell. 2016 Oct 10;39(1):13-27.
REF 39 Mitochondrial BCL-2 inhibits AMBRA1-induced autophagy. EMBO J. 2011 Apr 6;30(7):1195-208.
REF 40 The dynamic interaction of AMBRA1 with the dynein motor complex regulates mammalian autophagy. J Cell Biol. 2010 Oct 4;191(1):155-68.
REF 41 Ambra1 is an essential regulator of autophagy and apoptosis in SW620 cells: pro-survival role of Ambra1. PLoS One. 2014 Feb 26;9(2):e90151.
REF 42 AMBRA1 interplay with cullin E3 ubiquitin ligases regulates autophagy dynamics. Dev Cell. 2014 Dec 22;31(6):734-46.
REF 43 EGFR-targeted mAb therapy modulates autophagy in head and neck squamous cell carcinoma through NLRX1-TUFM protein complex. Oncogene. 2016 Sep 8;35(36):4698-707.
REF 44 OncoPPi-informed discovery of mitogen-activated protein kinase kinase 3 as a novel binding partner of c-Myc. Oncogene. 2017 Oct 19;36(42):5852-5860.

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