Target Interacting Protein Information

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Target General Information
Target Interacting Proteins
References
Target General Information  Top
Target ID T63220 Target Info
Target Name Activated CDC42 kinase 1 (ACK-1)
Synonyms Tyrosine kinase non-receptor protein 2; ACK1; ACK-1
Target Type Patented-recorded Target
Gene Name TNK2
Biochemical Class Kinase
UniProt ID
ACK1_HUMAN 
Target Interacting Proteins  Top
Acid anhydride hydrolases [+] 1 Acid anhydride hydrolases +
Interacting Protein Name Cell division control protein 42 homolog (CDC42) Interacting Protein Interacting Protein Info
UniProt ID
CDC42_HUMAN 
Synonyms CDC42Hs; G25K
Evidence Score (E-score) 8 Experimental Evidences +
1 Affinity Capture-Western [1]
2 Affinity Capture-Western [2]
3 Affinity Capture-Western [3]
4 Affinity Capture-Western [4]
5 Co-localization [3]
6 Fluorescence Resonance Energy Transfer [5]
7 Reconstituted Complex [6]
8 Reconstituted Complex [5]
Acyltransferases [+] 1 Acyltransferases +
Interacting Target Name E3 ubiquitin-protein ligase NEDD4 (NEDD4) Literature-reported Target Interacting Protein Info
UniProt ID
NEDD4_HUMAN 
Synonyms Neural precursor cell expressed developmentally down-regulated protein 4; NEDD4-1; NEDD-4; KIAA0093; HECT-type E3 ubiquitin transferase NEDD4; Cell proliferation-inducing gene 53 protein
Evidence Score (E-score) 3 Experimental Evidences +
1 Affinity Capture-Western [2]
2 Affinity Capture-Western [7]
3 Reconstituted Complex [2]
Adapter proteins [+] 1 Adapter proteins +
Interacting Protein Name Growth factor receptor-bound protein 2 (GRB2) Interacting Protein Interacting Protein Info
UniProt ID
GRB2_HUMAN 
Synonyms SH2/SH3 adapter GRB2; Protein Ash; Growth factor receptor-bound protein 2; ASH; Adapter protein GRB2
Evidence Score (E-score) 6 Experimental Evidences +
1 Affinity Capture-MS [8]
2 Affinity Capture-Western [9]
3 Affinity Capture-Western [2]
4 Reconstituted Complex [10]
5 Reconstituted Complex [11]
6 Yeast two hybrid [12]
Cell division cycle proteins [+] 1 Cell division cycle proteins +
Interacting Target Name Hsp90 co-chaperone Cdc37 (CDC37) Literature-reported Target Interacting Protein Info
UniProt ID
CDC37_HUMAN 
Synonyms p50Cdc37; Hsp90 co-chaperone Cdc37, N-terminally processed; Hsp90 chaperone protein kinase-targeting subunit; CDC37A
Evidence Score (E-score) 2 Experimental Evidences +
1 Affinity Capture-MS [8]
2 Affinity Capture-MS [13]
Heat shock proteins [+] 1 Heat shock proteins +
Interacting Target Name Heat shock protein 90 alpha (HSP90A) Successful Target Interacting Protein Info
UniProt ID
HS90A_HUMAN 
Synonyms Renal carcinoma antigen NY-REN-38; LPS-associated protein 2; Lipopolysaccharide-associated protein 2; LAP-2; HSPCA; HSPC1; HSP90A; HSP86; HSP 86; Heat shock protein HSP 90-alpha; Heat shock 86 kDa
Evidence Score (E-score) 5 Experimental Evidences +
1 Affinity Capture-Luminescence [14]
2 Affinity Capture-MS [15]
3 Affinity Capture-MS [8]
4 Affinity Capture-Western [15]
5 Affinity Capture-Western [8]
Kinases [+] 3 Kinases +
Interacting Target Name Epidermal growth factor receptor (EGFR) Successful Target Interacting Protein Info
UniProt ID
EGFR_HUMAN 
Synonyms Epidermal growth factor receptor variant III; Proto-oncogene c-ErbB-1 variant III; Receptor tyrosine-protein kinase erbB-1 variant III; EGFR variant III; ERBB variant III; ERBB1 variant III; HER1 variant III
Evidence Score (E-score) 5 Experimental Evidences +
1 Affinity Capture-MS [16]
2 Affinity Capture-Western [17]
3 Affinity Capture-Western [18]
4 Affinity Capture-Western [3]
5 Co-localization [3]
Interacting Target Name Proto-oncogene c-Src (SRC) Successful Target Interacting Protein Info
UniProt ID
SRC_HUMAN 
Synonyms Tyrosine kinase (pp60(src)); SRC1; Src tyrosine kinase; Proto-oncogene tyrosine-protein kinase Src; pp60c-src; Pp60(src); P60-Src; C-src TK; C-Src
Evidence Score (E-score) 2 Experimental Evidences +
1 Affinity Capture-Western [19]
2 Reconstituted Complex [20]
Interacting Target Name RAC-alpha serine/threonine-protein kinase (AKT1) Successful Target Interacting Protein Info
UniProt ID
AKT1_HUMAN 
Synonyms RAC-PK-alpha; RAC; Proto-oncogene c-Akt; Protein kinase B alpha; Protein kinase B; PKB alpha; PKB
Evidence Score (E-score) 2 Experimental Evidences +
1 Affinity Capture-Western [21]
2 Reconstituted Complex [22]
Other proteins [+] 2 Other proteins +
Interacting Protein Name Clathrin heavy chain 1 (CLTC) Interacting Protein Interacting Protein Info
UniProt ID
CLH1_HUMAN 
Synonyms CHC; CHC17; CLH.17; CLTCL2; Hc
Evidence Score (E-score) 3 Experimental Evidences +
1 Affinity Capture-MS [8]
2 Reconstituted Complex [23]
3 Reconstituted Complex [24]
Interacting Protein Name Nephrocystin-1 (NPHP1) Interacting Protein Interacting Protein Info
UniProt ID
NPHP1_HUMAN 
Synonyms JBTS4; NPH1; SLSN1
Evidence Score (E-score) 3 Experimental Evidences +
1 Affinity Capture-Western [25]
2 Reconstituted Complex [11]
3 Yeast two hybrid [25]
References  Top
REF 1 Epidermal growth factor stimulation of the ACK1/Dbl pathway in a Cdc42 and Grb2-dependent manner. Biochem Biophys Res Commun. 2001 Jun 8;284(2):470-7.
REF 2 HECT E3 ubiquitin ligase Nedd4-1 ubiquitinates ACK and regulates epidermal growth factor (EGF)-induced degradation of EGF receptor and ACK. Mol Cell Biol. 2010 Mar;30(6):1541-54.
REF 3 The non-receptor tyrosine kinase Ack1 regulates the fate of activated EGFR by inducing trafficking to the p62/NBR1 pre-autophagosome. J Cell Sci. 2014 Mar 1;127(Pt 5):994-1006.
REF 4 Expression, synaptic localization, and developmental regulation of Ack1/Pyk1, a cytoplasmic tyrosine kinase highly expressed in the developing and ... J Comp Neurol. 2005 Sep 19;490(2):119-32.
REF 5 A dock and coalesce mechanism driven by hydrophobic interactions governs Cdc42 binding with its effector protein ACK. J Biol Chem. 2017 Jul 7;292(27):11361-11373.
REF 6 Identification of the region in Cdc42 that confers the binding specificity to activated Cdc42-associated kinase. J Biol Chem. 2004 Jul 16;279(29):30507-13.
REF 7 Mutations in TNK2 in severe autosomal recessive infantile onset epilepsy. Ann Neurol. 2013 Sep;74(3):496-501.
REF 8 HSP90 is necessary for the ACK1-dependent phosphorylation of STAT1 and STAT3. Cell Signal. 2017 Nov;39:9-17.
REF 9 Tyrosine phosphorylation of ACK in response to temperature shift-down, hyperosmotic shock, and epidermal growth factor stimulation. FEBS Lett. 1996 May 20;386(2-3):230-4.
REF 10 The activation mechanism of ACK1 (activated Cdc42-associated tyrosine kinase 1). Biochem J. 2012 Jul 15;445(2):255-64.
REF 11 The Cdc42-associated kinase ACK1 is not autoinhibited but requires Src for activation. Biochem J. 2011 Apr 15;435(2):355-64.
REF 12 A human MAP kinase interactome. Nat Methods. 2010 Oct;7(10):801-5.
REF 13 Architecture of the human interactome defines protein communities and disease networks. Nature. 2017 May 25;545(7655):505-509.
REF 14 Quantitative analysis of HSP90-client interactions reveals principles of substrate recognition. Cell. 2012 Aug 31;150(5):987-1001.
REF 15 Activated tyrosine kinase Ack1 promotes prostate tumorigenesis: role of Ack1 in polyubiquitination of tumor suppressor Wwox. Cancer Res. 2005 Nov 15;65(22):10514-23.
REF 16 Tandem immunoprecipitation of phosphotyrosine-mass spectrometry (TIPY-MS) indicates C19ORF19 becomes tyrosine-phosphorylated and associated with activated epidermal growth factor receptor. J Proteome Res. 2008 Mar;7(3):1067-77.
REF 17 Cytoplasmic ACK1 interaction with multiple receptor tyrosine kinases is mediated by Grb2: an analysis of ACK1 effects on Axl signaling. J Biol Chem. 2009 Dec 11;284(50):34954-63.
REF 18 TNK2 preserves epidermal growth factor receptor expression on the cell surface and enhances migration and invasion of human breast cancer cells. Breast Cancer Res. 2008;10(2):R36.
REF 19 Ack1 mediates Cdc42-dependent cell migration and signaling to p130Cas. J Biol Chem. 2006 Dec 8;281(49):37527-35.
REF 20 Down-regulation of active ACK1 is mediated by association with the E3 ubiquitin ligase Nedd4-2. J Biol Chem. 2009 Mar 20;284(12):8185-94.
REF 21 Activated cdc42-associated kinase is up-regulated in non-small-cell lung cancer and necessary for FGFR-mediated AKT activation. Mol Carcinog. 2016 May;55(5):853-63.
REF 22 PDGFR--activated ACK1-AKT signaling promotes glioma tumorigenesis. Int J Cancer. 2015 Apr 15;136(8):1769-80.
REF 23 The tyrosine kinase ACK1 associates with clathrin-coated vesicles through a binding motif shared by arrestin and other adaptors. J Biol Chem. 2001 May 25;276(21):18392-8.
REF 24 Participation of Tom1L1 in EGF-stimulated endocytosis of EGF receptor. EMBO J. 2009 Nov 18;28(22):3485-99.
REF 25 Nephrocystin-1 interacts directly with Ack1 and is expressed in human collecting duct. Biochem Biophys Res Commun. 2008 Jul 11;371(4):877-82.

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