Target Interacting Protein Information

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Target General Information
Target Interacting Proteins
References
Target General Information  Top
Target ID T35940 Target Info
Target Name ERK activator kinase 1 (MEK1)
Synonyms PRKMK1; Mitogen-activated protein kinase kinase 1; MKK1; MEK 1; MAPKK 1; MAPK/ERKkinase 1; MAPK/ERK kinase 1; MAP kinase kinase 1; Dual specificity mitogen-activated protein kinase kinase 1
Target Type Clinical trial Target
Gene Name MAP2K1
Biochemical Class Kinase
UniProt ID
MP2K1_HUMAN 
Target Interacting Proteins  Top
Auxiliary transporters [+] 1 Auxiliary transporters +
Interacting Protein Name 14-3-3 protein zeta/delta (YWHAZ) Interacting Protein Interacting Protein Info
UniProt ID
1433Z_HUMAN 
Synonyms 14.3.3.zeta; HEL.S.3; HEL4; KCIP.1; YWHAD
Evidence Score (E-score) 2 Experimental Evidences +
1 Affinity Capture-MS [1]
2 Affinity Capture-MS [2]
Heat shock proteins [+] 1 Heat shock proteins +
Interacting Target Name Lipopolysaccharide-associated protein 1 (HSPA8) Literature-reported Target Interacting Protein Info
UniProt ID
HSP7C_HUMAN 
Synonyms LPS-associated protein 1; LAP-1; HSPA10; HSP73; HSC70; Heat shock protein 73; Heat shock cognate 71 kDa protein; Heat shock 70 kDa protein 8
Evidence Score (E-score) 2 Experimental Evidences +
1 Affinity Capture-MS [1]
2 Affinity Capture-MS [2]
Kinases [+] 6 Kinases +
Interacting Target Name Extracellular signal-regulated kinase 2 (ERK2) Clinical trial Target Interacting Protein Info
UniProt ID
MK01_HUMAN 
Synonyms PRKM2; PRKM1; P42-MAPK; P42 Mitogen-activated protein kinase; Mitogen-activated protein kinase 2; Mitogen-activated protein kinase 1; MAPK 2; MAPK 1; MAP kinase isoform p42; MAP kinase 2; MAP kinase 1; ERT1; ERK-2
Evidence Score (E-score) 7 Experimental Evidences +
1 Affinity Capture-Western [3]
2 Biochemical Activity [4]
3 Biochemical Activity [3]
4 Biochemical Activity [5]
5 Reconstituted Complex [6]
6 Reconstituted Complex [7]
7 Yeast two hybrid [8]
Interacting Target Name Proto-oncogene c-RAF (c-RAF) Clinical trial Target Interacting Protein Info
UniProt ID
RAF1_HUMAN 
Synonyms Raf-1; RAF proto-oncogene serine/threonine-protein kinase; RAF; cRaf
Evidence Score (E-score) 6 Experimental Evidences +
1 Affinity Capture-MS [1]
2 Affinity Capture-MS [9]
3 Affinity Capture-Western [10]
4 Affinity Capture-Western [11]
5 Reconstituted Complex [6]
6 Reconstituted Complex [11]
Interacting Target Name Extracellular signal-regulated kinase 1 (ERK1) Clinical trial Target Interacting Protein Info
UniProt ID
MK03_HUMAN 
Synonyms PRKM3; P44-MAPK; P44-ERK1; P44 Mitogen-activated protein kinase; Mitogen-activated protein kinase 3; Microtubule-associated protein-2 kinase; Microtubule-associated protein 2 kinase; MAPK 3; MAP kinase isoform p44; MAP kinase 3; Insulin-stimulated MAP2 kinase; ERT2; ERK-1
Evidence Score (E-score) 4 Experimental Evidences +
1 Biochemical Activity [12]
2 Biochemical Activity [13]
3 Reconstituted Complex [13]
4 Yeast two hybrid [14]
Interacting Target Name Serine/threonine-protein kinase B-raf (BRAF) Successful Target Interacting Protein Info
UniProt ID
BRAF_HUMAN 
Synonyms V-Raf murine sarcoma viral oncogene homolog B1; Serine/threonine-protein kinase B-raf; RAFB1; Proto-oncogene B-Raf; P94; BRAF1; BRAF(V599E); BRAF serine/threonine kinase; B-raf protein; B-Raf
Evidence Score (E-score) 3 Experimental Evidences +
1 Affinity Capture-MS [1]
2 Affinity Capture-MS [9]
3 Affinity Capture-Western [15]
Interacting Target Name Kinase suppressor of Ras-1 (KSR1) Literature-reported Target Interacting Protein Info
UniProt ID
KSR1_HUMAN 
Synonyms KSR; Kinase suppressor of Ras 1
Evidence Score (E-score) 3 Experimental Evidences +
1 Affinity Capture-MS [1]
2 Affinity Capture-MS [9]
3 Affinity Capture-Western [16]
Interacting Protein Name Proto-oncogene A-Raf (ARAF ) Interacting Protein Interacting Protein Info
UniProt ID
ARAF_HUMAN 
Synonyms Serine/threonine-protein kinase A-Raf; Proto-oncogene Pks; Proto-oncogene A-Raf-1; Proto-oncogene A-Raf; PKS2; PKS; ARAF1
Evidence Score (E-score) 2 Experimental Evidences +
1 Affinity Capture-MS [1]
2 Affinity Capture-MS [9]
Nuclear hormone receptors [+] 1 Nuclear hormone receptors +
Interacting Target Name Peroxisome proliferator-activated receptor gamma (PPAR-gamma) Successful Target Interacting Protein Info
UniProt ID
PPARG_HUMAN 
Synonyms Transcription factor PPAR gamma receptor; PPAR-gamma; PPARgamma; Peroxisome proliferator-activated receptor gamma; Nuclear receptor subfamily 1 group C member 3; NR1C3
Evidence Score (E-score) 3 Experimental Evidences +
1 Affinity Capture-Western [17]
2 Co-fractionation [17]
3 Reconstituted Complex [18]
Transcription regulators [+] 1 Transcription regulators +
Interacting Target Name Yes-associated protein 1 (YAP1) Literature-reported Target Interacting Protein Info
UniProt ID
YAP1_HUMAN 
Synonyms Yorkie homolog; Yes-associated protein YAP65 homolog; YAP65; Transcriptional coactivator YAP1; Protein yorkie homolog; 65 kDa Yes-associated protein
Evidence Score (E-score) 2 Experimental Evidences +
1 Affinity Capture-MS [2]
2 Affinity Capture-Western [19]
References  Top
REF 1 A novel tandem affinity purification strategy for the efficient isolation and characterisation of native protein complexes. Proteomics. 2007 Dec;7(23):4228-34.
REF 2 Genetic and Proteomic Interrogation of Lower Confidence Candidate Genes Reveals Signaling Networks in -Catenin-Active Cancers. Cell Syst. 2016 Sep 28;3(3):302-316.e4.
REF 3 Hydrophobic as well as charged residues in both MEK1 and ERK2 are important for their proper docking. J Biol Chem. 2001 Jul 13;276(28):26509-15.
REF 4 p38alpha isoform Mxi2 binds to extracellular signal-regulated kinase 1 and 2 mitogen-activated protein kinase and regulates its nuclear activity by sustaining its phosphorylation levels. Mol Cell Biol. 2003 May;23(9):3079-90.
REF 5 Regulation of stress-responsive mitogen-activated protein (MAP) kinase pathways by TAO2. J Biol Chem. 2001 May 11;276(19):16070-5.
REF 6 Mechanism of suppression of the Raf/MEK/extracellular signal-regulated kinase pathway by the raf kinase inhibitor protein. Mol Cell Biol. 2000 May;20(9):3079-85.
REF 7 A docking site in MKK4 mediates high affinity binding to JNK MAPKs and competes with similar docking sites in JNK substrates. J Biol Chem. 2003 Aug 29;278(35):32662-72.
REF 8 Identification of novel point mutations in ERK2 that selectively disrupt binding to MEK1. J Biol Chem. 2002 Apr 26;277(17):14844-52.
REF 9 A human interactome in three quantitative dimensions organized by stoichiometries and abundances. Cell. 2015 Oct 22;163(3):712-23.
REF 10 Identification of Raf-1 S471 as a novel phosphorylation site critical for Raf-1 and B-Raf kinase activities and for MEK binding. Mol Biol Cell. 2005 Oct;16(10):4733-44.
REF 11 Disruption of the Rb--Raf-1 interaction inhibits tumor growth and angiogenesis. Mol Cell Biol. 2004 Nov;24(21):9527-41.
REF 12 Characterization of ERK1 activation site mutants and the effect on recognition by MEK1 and MEK2. J Biol Chem. 1996 Feb 23;271(8):4230-5.
REF 13 Properties of MEKs, the kinases that phosphorylate and activate the extracellular signal-regulated kinases. J Biol Chem. 1993 Nov 15;268(32):23933-9.
REF 14 Actin-binding protein-280 binds the stress-activated protein kinase (SAPK) activator SEK-1 and is required for tumor necrosis factor-alpha activation of SAPK in melanoma cells. J Biol Chem. 1997 Jan 31;272(5):2620-8.
REF 15 Glucocorticoids cause rapid dissociation of a T-cell-receptor-associated protein complex containing LCK and FYN. EMBO Rep. 2006 Oct;7(10):1023-9.
REF 16 Leukocyte-specific protein 1 targets the ERK/MAP kinase scaffold protein KSR and MEK1 and ERK2 to the actin cytoskeleton. J Cell Sci. 2004 Apr 15;117(Pt 10):2151-7.
REF 17 The Ras inhibitors caveolin-1 and docking protein 1 activate peroxisome proliferator-activated receptor through spatial relocalization at helix 7 of its ligand-binding domain. Mol Cell Biol. 2011 Aug;31(16):3497-510.
REF 18 Interaction with MEK causes nuclear export and downregulation of peroxisome proliferator-activated receptor gamma. Mol Cell Biol. 2007 Feb;27(3):803-17.
REF 19 MEK1 promotes YAP and their interaction is critical for tumorigenesis in liver cancer. FEBS Lett. 2013 Dec 11;587(24):3921-7.

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