Target Interacting Protein Information

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Target General Information
Target Interacting Proteins
References
Target General Information  Top
Target ID T35799 Target Info
Target Name DNA mismatch repair protein Mlh1 (MLH1)
Synonyms MutL protein homolog 1; COCA2
Target Type Literature-reported Target
Gene Name MLH1
UniProt ID
MLH1_HUMAN 
Target Interacting Proteins  Top
Acid anhydride hydrolases [+] 1 Acid anhydride hydrolases +
Interacting Protein Name Bloom syndrome protein (BLM) Interacting Protein Interacting Protein Info
UniProt ID
BLM_HUMAN 
Synonyms BS; RECQ2; RECQL2; RECQL3
Evidence Score (E-score) 4 Experimental Evidences +
1 Affinity Capture-Western [1]
2 Co-purification [2]
3 Protein-peptide [3]
4 Yeast two hybrid [3]
Ester hydrolases [+] 7 Ester hydrolases +
Interacting Protein Name Mismatch repair endonuclease PMS2 (PMS2) Interacting Protein Interacting Protein Info
UniProt ID
PMS2_HUMAN 
Synonyms HNPCC4; PMS2CL; PMSL2
Evidence Score (E-score) 17 Experimental Evidences +
1 Affinity Capture-MS [4]
2 Affinity Capture-Western [4]
3 Affinity Capture-Western [5]
4 Affinity Capture-Western [6]
5 Affinity Capture-Western [7]
6 Affinity Capture-Western [8]
7 Co-fractionation [9]
8 Reconstituted Complex [10]
9 Reconstituted Complex [11]
10 Reconstituted Complex [12]
11 Reconstituted Complex [13]
12 Reconstituted Complex [14]
13 Reconstituted Complex [15]
14 Yeast two hybrid [10]
15 Yeast two hybrid [16]
16 Yeast two hybrid [3]
17 Yeast two hybrid [12]
Interacting Protein Name Exonuclease 1 (EXO1) Interacting Protein Interacting Protein Info
UniProt ID
EXO1_HUMAN 
Synonyms HEX1; hExoI
Evidence Score (E-score) 6 Experimental Evidences +
1 Affinity Capture-MS [4]
2 Affinity Capture-Western [17]
3 Protein-peptide [3]
4 Reconstituted Complex [3]
5 Reconstituted Complex [18]
6 Reconstituted Complex [11]
Interacting Target Name DNA mismatch repair protein PMS1 (PMS1) Literature-reported Target Interacting Protein Info
UniProt ID
PMS1_HUMAN 
Synonyms PMSL1; PMS1 protein homolog 1
Evidence Score (E-score) 4 Experimental Evidences +
1 Affinity Capture-MS [4]
2 Affinity Capture-MS [19]
3 Affinity Capture-Western [5]
4 Co-localization [20]
Interacting Target Name DNA mismatch repair protein MSH2 (MSH2) Literature-reported Target Interacting Protein Info
UniProt ID
MSH2_HUMAN 
Synonyms MutS protein homolog 2; Mismatch repair gene Msh2; hMSH2
Evidence Score (E-score) 3 Experimental Evidences +
1 Affinity Capture-Western [8]
2 Co-localization [21]
3 Co-purification [2]
Interacting Protein Name Fanconi-associated nuclease 1 (FAN1) Interacting Protein Interacting Protein Info
UniProt ID
FAN1_HUMAN 
Synonyms KIAA1018; KMIN; MTMR15
Evidence Score (E-score) 2 Experimental Evidences +
1 Affinity Capture-MS [4]
2 Affinity Capture-Western [22]
Interacting Protein Name DNA mismatch repair protein Mlh3 (MLH3) Interacting Protein Interacting Protein Info
UniProt ID
MLH3_HUMAN 
Synonyms HNPCC7
Evidence Score (E-score) 2 Experimental Evidences +
1 Reconstituted Complex [23]
2 Yeast two hybrid [24]
Interacting Protein Name DNA mismatch repair protein Msh3 (MSH3) Interacting Protein Interacting Protein Info
UniProt ID
MSH3_HUMAN 
Synonyms DUP; MRP1
Evidence Score (E-score) 2 Experimental Evidences +
1 Affinity Capture-MS [4]
2 Yeast two hybrid [25]
Oxidoreductases [+] 1 Oxidoreductases +
Interacting Protein Name Programmed cell death protein 8 (AIFM1) Interacting Protein Interacting Protein Info
UniProt ID
AIFM1_HUMAN 
Synonyms AIF; CMT2D; CMTX4; COWCK; COXPD6; NADMR; NAMSD; PDCD8
Evidence Score (E-score) 2 Experimental Evidences +
1 Affinity Capture-MS [4]
2 Yeast two hybrid [16]
Other proteins [+] 1 Other proteins +
Interacting Protein Name Alpha-II spectrin (SPTAN1) Interacting Protein Interacting Protein Info
UniProt ID
SPTN1_HUMAN 
Synonyms EIEE5; NEAS; SPTA2
Evidence Score (E-score) 3 Experimental Evidences +
1 Affinity Capture-Western [16]
2 Co-fractionation [26]
3 Yeast two hybrid [16]
References  Top
REF 1 Direct association of Bloom's syndrome gene product with the human mismatch repair protein MLH1. Nucleic Acids Res. 2001 Nov 1;29(21):4378-86.
REF 2 BASC, a super complex of BRCA1-associated proteins involved in the recognition and repair of aberrant DNA structures. Genes Dev. 2000 Apr 15;14(8):927-39.
REF 3 Characterization of a highly conserved binding site of Mlh1 required for exonuclease I-dependent mismatch repair. Mol Cell Biol. 2009 Feb;29(3):907-18.
REF 4 Characterization of the interactome of the human MutL homologues MLH1, PMS1, and PMS2. J Biol Chem. 2007 Feb 2;282(5):2976-86.
REF 5 Identification of mismatch repair protein complexes in HeLa nuclear extracts and their interaction with heteroduplex DNA. J Biol Chem. 2000 Jun 9;275(23):17808-13.
REF 6 An MLH1 mutation links BACH1/FANCJ to colon cancer, signaling, and insight toward directed therapy. Cancer Prev Res (Phila). 2010 Nov;3(11):1409-16.
REF 7 The FANCJ/MutLalpha interaction is required for correction of the cross-link response in FA-J cells. EMBO J. 2007 Jul 11;26(13):3238-49.
REF 8 N-terminus of hMLH1 confers interaction of hMutLalpha and hMutLbeta with hMutSalpha. Nucleic Acids Res. 2003 Jun 15;31(12):3217-26.
REF 9 A census of human soluble protein complexes. Cell. 2012 Aug 31;150(5):1068-81.
REF 10 The interacting domains of three MutL heterodimers in man: hMLH1 interacts with 36 homologous amino acid residues within hMLH3, hPMS1 and hPMS2. Nucleic Acids Res. 2001 Apr 15;29(8):1695-702.
REF 11 HNPCC mutations in the human DNA mismatch repair gene hMLH1 influence assembly of hMutLalpha and hMLH1-hEXO1 complexes. Oncogene. 2001 Jun 14;20(27):3590-5.
REF 12 Interactions of the DNA mismatch repair proteins MLH1 and MSH2 with c-MYC and MAX. Oncogene. 2003 Feb 13;22(6):819-25.
REF 13 The interaction of the human MutL homologues in hereditary nonpolyposis colon cancer. J Biol Chem. 1999 Mar 5;274(10):6336-41.
REF 14 A defined human system that supports bidirectional mismatch-provoked excision. Mol Cell. 2004 Jul 2;15(1):31-41.
REF 15 Direct visualization of asymmetric adenine-nucleotide-induced conformational changes in MutL alpha. Mol Cell. 2008 Jan 18;29(1):112-21.
REF 16 Cytoskeletal scaffolding proteins interact with Lynch-Syndrome associated mismatch repair protein MLH1. Proteomics. 2010 Sep;10(18):3343-55.
REF 17 14-3-3 Proteins regulate exonuclease 1-dependent processing of stalled replication forks. PLoS Genet. 2011 Apr;7(4):e1001367.
REF 18 14-3-3 checkpoint regulatory proteins interact specifically with DNA repair protein human exonuclease 1 (hEXO1) via a semi-conserved motif. DNA Repair (Amst). 2012 Mar 1;11(3):267-77.
REF 19 A human interactome in three quantitative dimensions organized by stoichiometries and abundances. Cell. 2015 Oct 22;163(3):712-23.
REF 20 Nuclear reorganization of DNA mismatch repair proteins in response to DNA damage. DNA Repair (Amst). 2010 Feb 4;9(2):120-33.
REF 21 Using an in situ proximity ligation assay to systematically profile endogenous protein-protein interactions in a pathway network. J Proteome Res. 2014 Dec 5;13(12):5339-46.
REF 22 A genetic screen identifies FAN1, a Fanconi anemia-associated nuclease necessary for DNA interstrand crosslink repair. Mol Cell. 2010 Jul 9;39(1):36-47.
REF 23 The Saccharomyces cerevisiae Mlh1-Mlh3 heterodimer is an endonuclease that preferentially binds to Holliday junctions. J Biol Chem. 2014 Feb 28;289(9):5674-86.
REF 24 Systematic interactome mapping of acute lymphoblastic leukemia cancer gene products reveals EXT-1 tumor suppressor as a Notch1 and FBWX7 common interactor. BMC Cancer. 2016 May 26;16:335.
REF 25 A proteome-scale map of the human interactome network. Cell. 2014 Nov 20;159(5):1212-1226.
REF 26 Panorama of ancient metazoan macromolecular complexes. Nature. 2015 Sep 17;525(7569):339-44.

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