Target Interacting Protein Information

Content Navigation (  All   None )                                      
Target General Information
Target Interacting Proteins
References
Target General Information  Top
Target ID T09826 Target Info
Target Name DNA topoisomerase I (TOP1)
Synonyms DNA topoisomerase I
Target Type Successful Target
Gene Name TOP1
Biochemical Class Topoisomerase
UniProt ID
TOP1_HUMAN 
Target Interacting Proteins  Top
Acid anhydride hydrolases [+] 1 Acid anhydride hydrolases +
Interacting Target Name X-ray repair cross-complementing 5 (Ku80) Literature-reported Target Interacting Protein Info
UniProt ID
XRCC5_HUMAN 
Synonyms X-ray repair cross-complementing protein 5; X-ray repair complementing defective repair in Chinese hamster cells 5 (double-strand-break rejoining); TLAA; Thyroid-lupus autoantigen; Nuclear factor IV; Lupus Ku autoantigen protein p86; Ku86; G22P2; DNA repair protein XRCC5; CTCBF; CTC85; CTC box-binding factor 85 kDa subunit; ATP-dependent DNA helicase II 80 kDa subunit; ATP-dependent DNA helicase 2 subunit 2; 86 kDa subunit of Ku antigen
Evidence Score (E-score) 2 Experimental Evidences +
1 Affinity Capture-MS [1]
2 Affinity Capture-Western [2]
Cell cycle related proteins [+] 1 Cell cycle related proteins +
Interacting Target Name Multiple tumor suppressor 1 (CDKN2A) Literature-reported Target Interacting Protein Info
UniProt ID
CDN2A_HUMAN 
Synonyms Tumour suppressor gene p16; P16-INK4a; p16INK4A; P16-INK4; P16 gene; MTS-1; Cyclin-dependent kinase inhibitor 2A; Cyclin-dependent kinase 4 inhibitor A; CDK4I
Evidence Score (E-score) 2 Experimental Evidences +
1 Affinity Capture-Western [3]
2 Co-localization [4]
Pore-forming toxins [+] 1 Pore-forming toxins +
Interacting Target Name Cellular tumor antigen p53 (TP53) Clinical trial Target Interacting Protein Info
UniProt ID
P53_HUMAN 
Synonyms Tumor suppressor p53; Phosphoprotein p53; P53; Antigen NY-CO-13
Evidence Score (E-score) 2 Experimental Evidences +
1 Affinity Capture-Western [5]
2 Yeast two hybrid [6]
Splicing factors [+] 1 Splicing factors +
Interacting Protein Name Serine/arginine-rich splicing factor 1 (SRSF1) Interacting Protein Interacting Protein Info
UniProt ID
SRSF1_HUMAN 
Synonyms ASF; SF2; SF2p33; SFRS1; SRp30a
Evidence Score (E-score) 3 Experimental Evidences +
1 Affinity Capture-MS [7]
2 Far Western [8]
3 Reconstituted Complex [9]
Ubiquitin related proteins [+] 1 Ubiquitin related proteins +
Interacting Target Name Ubiquitin-protein ligase E3 Mdm2 (MDM2) Clinical trial Target Interacting Protein Info
UniProt ID
MDM2_HUMAN 
Synonyms RING-type E3 ubiquitin transferase Mdm2; P53-binding protein Mdm2; Oncoprotein Mdm2; MDM2 protein; Hdm2; E3 ubiquitin-protein ligase Mdm2; Double minute 2 protein
Evidence Score (E-score) 2 Experimental Evidences +
1 Affinity Capture-MS [10]
2 Protein-peptide [11]
Other proteins [+] 4 Other proteins +
Interacting Target Name Poly [ADP-ribose] polymerase 1 (PARP1) Successful Target Interacting Protein Info
UniProt ID
PARP1_HUMAN 
Synonyms Protein poly-ADP-ribosyltransferase PARP1; PPOL; Poly[ADP-ribose] synthetase-1; Poly[ADP-ribose] synthase 1; Poly(ADP-ribose)polymerase-1; PARP-1; NAD(+)Poly [ADP-ribose] polymerase-1 ADP-ribosyltransferase-1; NAD(+) ADP-ribosyltransferase-1; NAD(+) ADP-ribosyltransferase 1; DNA ADP-ribosyltransferase PARP1; ARTD1; ADPRT 1; ADPRT; ADP-ribosyltransferase diphtheria toxin-like 1
Evidence Score (E-score) 5 Experimental Evidences +
1 Affinity Capture-MS [7]
2 Affinity Capture-Western [12]
3 Co-fractionation [12]
4 Fluorescence Resonance Energy Transfer [12]
5 Reconstituted Complex [12]
Interacting Target Name Nucleolin (NCL) Clinical trial Target Interacting Protein Info
UniProt ID
NUCL_HUMAN 
Synonyms Protein C23; Nucleolin
Evidence Score (E-score) 3 Experimental Evidences +
1 Affinity Capture-MS [7]
2 Affinity Capture-Western [13]
3 Reconstituted Complex [13]
Interacting Target Name Spliceosome-associated protein 155 (SF3B1) Clinical trial Target Interacting Protein Info
UniProt ID
SF3B1_HUMAN 
Synonyms Spliceosome-associated protein 155; SF3b155; SAP155; SAP 155; Pre-mRNA-splicing factor SF3b 155 kDa subunit
Evidence Score (E-score) 2 Experimental Evidences +
1 Affinity Capture-MS [7]
2 Protein-peptide [14]
Interacting Protein Name PTB-associated-splicing factor (SFPQ) Interacting Protein Interacting Protein Info
UniProt ID
SFPQ_HUMAN 
Synonyms POMP100; PPP1R140; PSF
Evidence Score (E-score) 2 Experimental Evidences +
1 Affinity Capture-MS [7]
2 Affinity Capture-Western [15]
References  Top
REF 1 Concordant and opposite roles of DNA-PK and the "facilitator of chromatin transcription" (FACT) in DNA repair, apoptosis and necrosis after cisplatin. Mol Cancer. 2011 Jun 16;10:74.
REF 2 Camptothecin resistance is determined by the regulation of topoisomerase I degradation mediated by ubiquitin proteasome pathway. Oncotarget. 2017 Jul 4;8(27):43733-43751.
REF 3 Human Arf tumor suppressor specifically interacts with chromatin containing the promoter of rRNA genes. Oncogene. 2004 Oct 21;23(49):8097-104.
REF 4 Delineation of the domains required for physical and functional interaction of p14ARF with human topoisomerase I. Oncogene. 2003 Apr 3;22(13):1945-54.
REF 5 The interaction between p53 and DNA topoisomerase I is regulated differently in cells with wild-type and mutant p53. Proc Natl Acad Sci U S A. 1999 Aug 31;96(18):10355-60.
REF 6 Subnuclear distribution of topoisomerase I is linked to ongoing transcription and p53 status. Proc Natl Acad Sci U S A. 2002 Feb 5;99(3):1235-40.
REF 7 Proteomic analysis of complexes formed by human topoisomerase I. Biochim Biophys Acta. 2005 May 20;1749(1):133-41.
REF 8 Interaction between the N-terminal domain of human DNA topoisomerase I and the arginine-serine domain of its substrate determines phosphorylation of SF2/ASF splicing factor. Nucleic Acids Res. 1998 Jun 15;26(12):2955-62.
REF 9 The RNA splicing factor ASF/SF2 inhibits human topoisomerase I mediated DNA relaxation. J Mol Biol. 2002 Sep 27;322(4):677-86.
REF 10 Endogenous human MDM2-C is highly expressed in human cancers and functions as a p53-independent growth activator. PLoS One. 2013 Oct 11;8(10):e77643.
REF 11 A systems wide mass spectrometric based linear motif screen to identify dominant in-vivo interacting proteins for the ubiquitin ligase MDM2. Cell Signal. 2014 Jun;26(6):1243-57.
REF 12 Poly(ADP-ribosyl)ation as a DNA damage-induced post-translational modification regulating poly(ADP-ribose) polymerase-1-topoisomerase I interaction. J Biol Chem. 2004 Sep 17;279(38):39686-96.
REF 13 Identification of a nucleolin binding site in human topoisomerase I. J Biol Chem. 1996 Jan 26;271(4):1993-7.
REF 14 Potential protein partners for the N-terminal domain of human topoisomerase I revealed by phage display. Mol Biol Rep. 2002 Dec;29(4):347-52.
REF 15 The RNA-splicing factor PSF/p54 controls DNA-topoisomerase I activity by a direct interaction. J Biol Chem. 1998 Oct 9;273(41):26261-4.

If You Find Any Error in Data or Bug in Web Service, Please Kindly Report It to Dr. Zhou and Dr. Zhang.