Target Interacting Protein Information

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Target General Information
Target Interacting Proteins
References
Target General Information  Top
Target ID T01283 Target Info
Target Name Tumor susceptibility gene protein 101 (TSG101)
Synonyms Tumor susceptibility gene 101 protein; ESCRT-I complex subunit TSG101
Target Type Clinical trial Target
Gene Name TSG101
Biochemical Class Ubiquitin-conjugating enzyme
UniProt ID
TS101_HUMAN 
Target Interacting Proteins  Top
Acyltransferases [+] 2 Acyltransferases +
Interacting Protein Name E3 ubiquitin-protein ligase LRSAM1 (LRSAM1) Interacting Protein Interacting Protein Info
UniProt ID
LRSM1_HUMAN 
Synonyms CMT2P; RIFLE; TAL
Evidence Score (E-score) 9 Experimental Evidences +
1 Affinity Capture-MS [1]
2 Affinity Capture-Western [2]
3 Reconstituted Complex [3]
4 Reconstituted Complex [2]
5 Yeast two hybrid [3]
6 Yeast two hybrid [4]
7 Yeast two hybrid [5]
8 Yeast two hybrid [6]
9 Yeast two hybrid [7]
Interacting Protein Name E3 ubiquitin-protein ligase MGRN1 (MGRN1) Interacting Protein Interacting Protein Info
UniProt ID
MGRN1_HUMAN 
Synonyms RNF156
Evidence Score (E-score) 6 Experimental Evidences +
1 Affinity Capture-Western [8]
2 Affinity Capture-Western [9]
3 Reconstituted Complex [8]
4 Yeast two hybrid [8]
5 Yeast two hybrid [4]
6 Yeast two hybrid [6]
Adapter proteins [+] 1 Adapter proteins +
Interacting Protein Name Growth factor receptor-bound protein 2 (GRB2) Interacting Protein Interacting Protein Info
UniProt ID
GRB2_HUMAN 
Synonyms SH2/SH3 adapter GRB2; Protein Ash; Growth factor receptor-bound protein 2; ASH; Adapter protein GRB2
Evidence Score (E-score) 1 Experimental Evidences +
1 Affinity Capture-MS [10]
Nuclear hormone receptors [+] 2 Nuclear hormone receptors +
Interacting Target Name Androgen receptor (AR) Successful Target Interacting Protein Info
UniProt ID
ANDR_HUMAN 
Synonyms Testosterone receptor; Nuclear receptor subfamily 3 group C member 4; NR3C4; Dihydrotestosterone receptor; DHTR; Androgen receptor
Evidence Score (E-score) 2 Experimental Evidences +
1 Co-localization [11]
2 Yeast two hybrid [12]
Interacting Target Name Glucocorticoid receptor (NR3C1) Successful Target Interacting Protein Info
UniProt ID
GCR_HUMAN 
Synonyms Nuclear receptor subfamily 3 group C member 1; GRL; GR; Glucocorticoid receptor; Alpha-A
Evidence Score (E-score) 2 Experimental Evidences +
1 Affinity Capture-Western [13]
2 Yeast two hybrid [12]
Ubiquitin related proteins [+] 2 Ubiquitin related proteins +
Interacting Protein Name Ubiquitin-associated protein 1 (UBAP1) Interacting Protein Interacting Protein Info
UniProt ID
UBAP1_HUMAN 
Synonyms NAG20; UAP; UBAP; UBAP.1
Evidence Score (E-score) 6 Experimental Evidences +
1 Affinity Capture-MS [14]
2 Affinity Capture-Western [15]
3 Affinity Capture-Western [16]
4 Affinity Capture-Western [17]
5 Co-fractionation [18]
6 Reconstituted Complex [16]
Interacting Target Name Polyubiquitin-C (UBC) Patented-recorded Target Interacting Protein Info
UniProt ID
UBC_HUMAN 
Synonyms HMG20; ubiquitin C
Evidence Score (E-score) 3 Experimental Evidences +
1 Affinity Capture-MS [1]
2 Co-crystal Structure [19]
3 Co-crystal Structure [20]
Other proteins [+] 9 Other proteins +
Interacting Protein Name Hepatocyte growth factor-regulated tyrosine kinase substrate (HGS) Interacting Protein Interacting Protein Info
UniProt ID
HGS_HUMAN 
Synonyms HRS
Evidence Score (E-score) 12 Experimental Evidences +
1 Affinity Capture-MS [1]
2 Affinity Capture-Western [21]
3 Affinity Capture-Western [22]
4 Co-localization [22]
5 Protein-peptide [23]
6 Protein-peptide [1]
7 Reconstituted Complex [24]
8 Yeast two hybrid [21]
9 Yeast two hybrid [5]
10 Yeast two hybrid [24]
11 Yeast two hybrid [25]
12 Yeast two hybrid [26]
Interacting Protein Name Vacuolar protein sorting-associated protein 28 homolog (VPS28) Interacting Protein Interacting Protein Info
UniProt ID
VPS28_HUMAN 
Evidence Score (E-score) 8 Experimental Evidences +
1 Affinity Capture-MS [14]
2 Affinity Capture-Western [27]
3 Co-fractionation [18]
4 Reconstituted Complex [28]
5 Reconstituted Complex [16]
6 Yeast two hybrid [29]
7 Yeast two hybrid [4]
8 Yeast two hybrid [5]
Interacting Protein Name Centrosomal protein of 55 kDa (CEP55) Interacting Protein Interacting Protein Info
UniProt ID
CEP55_HUMAN 
Synonyms C10orf3; CT111; URCC6
Evidence Score (E-score) 7 Experimental Evidences +
1 Affinity Capture-MS [30]
2 Affinity Capture-Western [30]
3 Yeast two hybrid [30]
4 Yeast two hybrid [4]
5 Yeast two hybrid [31]
6 Yeast two hybrid [5]
7 Yeast two hybrid [32]
Interacting Protein Name Vacuolar protein sorting-associated protein 37A (VPS37A) Interacting Protein Interacting Protein Info
UniProt ID
VP37A_HUMAN 
Synonyms HCRP1; PQBP2; SPG53
Evidence Score (E-score) 7 Experimental Evidences +
1 Affinity Capture-MS [14]
2 Affinity Capture-Western [15]
3 Affinity Capture-Western [17]
4 Reconstituted Complex [16]
5 Yeast two hybrid [29]
6 Yeast two hybrid [4]
7 Yeast two hybrid [33]
Interacting Protein Name Vacuolar protein sorting-associated protein 37B (VPS37B) Interacting Protein Interacting Protein Info
UniProt ID
VP37B_HUMAN 
Evidence Score (E-score) 6 Experimental Evidences +
1 Affinity Capture-MS [14]
2 Affinity Capture-Western [17]
3 Co-fractionation [18]
4 Co-fractionation [34]
5 Protein-peptide [1]
6 Yeast two hybrid [29]
Interacting Protein Name Vacuolar protein sorting-associated protein 37C (VPS37C) Interacting Protein Interacting Protein Info
UniProt ID
VP37C_HUMAN 
Evidence Score (E-score) 6 Experimental Evidences +
1 Affinity Capture-MS [14]
2 Affinity Capture-Western [15]
3 Co-fractionation [18]
4 Reconstituted Complex [16]
5 Yeast two hybrid [35]
6 Yeast two hybrid [4]
Interacting Protein Name Programmed cell death 6-interacting protein (PDCD6IP) Interacting Protein Interacting Protein Info
UniProt ID
PDC6I_HUMAN 
Synonyms AIP1; ALIX; DRIP4; HP95
Evidence Score (E-score) 4 Experimental Evidences +
1 Affinity Capture-MS [1]
2 Affinity Capture-Western [1]
3 Affinity Capture-Western [36]
4 Protein-peptide [1]
Interacting Protein Name Vacuolar protein-sorting-associated protein 36 (VPS36) Interacting Protein Interacting Protein Info
UniProt ID
VPS36_HUMAN 
Synonyms C13orf9; EAP45
Evidence Score (E-score) 3 Experimental Evidences +
1 Affinity Capture-Western [22]
2 Co-localization [22]
3 Yeast two hybrid [37]
Interacting Protein Name Multivesicular body subunit 12A (MVB12A) Interacting Protein Interacting Protein Info
UniProt ID
MB12A_HUMAN 
Synonyms CFBP; FAM125A
Evidence Score (E-score) 2 Experimental Evidences +
1 Affinity Capture-MS [14]
2 Reconstituted Complex [16]
References  Top
REF 1 Proline-rich sequence recognition: II. Proteomics analysis of Tsg101 ubiquitin-E2-like variant (UEV) interactions. Mol Cell Proteomics. 2009 Nov;8(11):2474-86.
REF 2 TSG154-1054 splice variant increases TSG101 oncogenicity by inhibiting its E3-ligase-mediated proteasomal degradation. Oncotarget. 2016 Feb 16;7(7):8240-52.
REF 3 Tal, a Tsg101-specific E3 ubiquitin ligase, regulates receptor endocytosis and retrovirus budding. Genes Dev. 2004 Jul 15;18(14):1737-52.
REF 4 A proteome-scale map of the human interactome network. Cell. 2014 Nov 20;159(5):1212-1226.
REF 5 Towards a proteome-scale map of the human protein-protein interaction network. Nature. 2005 Oct 20;437(7062):1173-8.
REF 6 Analysis of the human E2 ubiquitin conjugating enzyme protein interaction network. Genome Res. 2009 Oct;19(10):1905-11.
REF 7 Regulation of Tsg101 expression by the steadiness box: a role of Tsg101-associated ligase. Mol Biol Cell. 2008 Feb;19(2):754-63.
REF 8 Spongiform neurodegeneration-associated E3 ligase Mahogunin ubiquitylates TSG101 and regulates endosomal trafficking. Mol Biol Cell. 2007 Apr;18(4):1129-42.
REF 9 Abnormal regulation of TSG101 in mice with spongiform neurodegeneration. Biochim Biophys Acta. 2009 Oct;1792(10):1027-35.
REF 10 Selected reaction monitoring mass spectrometry reveals the dynamics of signaling through the GRB2 adaptor. Nat Biotechnol. 2011 Jun 26;29(7):653-8.
REF 11 Zipper-interacting protein kinase is involved in regulation of ubiquitination of the androgen receptor, thereby contributing to dynamic transcription complex assembly. Oncogene. 2013 Oct 10;32(41):4981-8.
REF 12 Differential regulation of glucocorticoid receptor transcriptional activation via AF-1-associated proteins. EMBO J. 1999 Oct 1;18(19):5380-8.
REF 13 Stabilization of the unliganded glucocorticoid receptor by TSG101. J Biol Chem. 2005 Mar 25;280(12):11120-6.
REF 14 TNIP2 is a Hub Protein in the NF-B Network with Both Protein and RNA Mediated Interactions. Mol Cell Proteomics. 2016 Nov;15(11):3435-3449.
REF 15 UBAP1 is a component of an endosome-specific ESCRT-I complex that is essential for MVB sorting. Curr Biol. 2011 Jul 26;21(14):1245-50.
REF 16 The molecular basis for selective assembly of the UBAP1-containing endosome-specific ESCRT-I complex. J Cell Sci. 2014 Feb 1;127(Pt 3):663-72.
REF 17 The UBAP1 subunit of ESCRT-I interacts with ubiquitin via a SOUBA domain. Structure. 2012 Mar 7;20(3):414-28.
REF 18 Panorama of ancient metazoan macromolecular complexes. Nature. 2015 Sep 17;525(7569):339-44.
REF 19 Ubiquitin recognition by the human TSG101 protein. Mol Cell. 2004 Mar 26;13(6):783-9.
REF 20 Structure and functional interactions of the Tsg101 UEV domain. EMBO J. 2002 May 15;21(10):2397-406.
REF 21 TSG101 interaction with HRS mediates endosomal trafficking and receptor down-regulation. Proc Natl Acad Sci U S A. 2003 Jun 24;100(13):7626-31.
REF 22 ESCRT-I Protein Tsg101 Plays a Role in the Post-macropinocytic Trafficking and Infection of Endothelial Cells by Kaposi's Sarcoma-Associated Herpesvirus. PLoS Pathog. 2016 Oct 20;12(10):e1005960.
REF 23 Crystallographic and functional analysis of the ESCRT-I /HIV-1 Gag PTAP interaction. Structure. 2010 Nov 10;18(11):1536-47.
REF 24 HIV Gag mimics the Tsg101-recruiting activity of the human Hrs protein. J Cell Biol. 2003 Aug 4;162(3):425-34.
REF 25 Hrs regulates multivesicular body formation via ESCRT recruitment to endosomes. J Cell Biol. 2003 Aug 4;162(3):435-42.
REF 26 The C-terminal portion of the Hrs protein interacts with Tsg101 and interferes with human immunodeficiency virus type 1 Gag particle production. J Virol. 2007 Mar;81(6):2909-22.
REF 27 Role of ESCRT-I in retroviral budding. J Virol. 2003 Apr;77(8):4794-804.
REF 28 TSG101/mammalian VPS23 and mammalian VPS28 interact directly and are recruited to VPS4-induced endosomes. J Biol Chem. 2001 Apr 13;276(15):11735-42.
REF 29 The human endosomal sorting complex required for transport (ESCRT-I) and its role in HIV-1 budding. J Biol Chem. 2004 Aug 20;279(34):36059-71.
REF 30 Human ESCRT and ALIX proteins interact with proteins of the midbody and function in cytokinesis. EMBO J. 2007 Oct 3;26(19):4215-27.
REF 31 Pooled-matrix protein interaction screens using Barcode Fusion Genetics. Mol Syst Biol. 2016 Apr 22;12(4):863.
REF 32 rec-YnH enables simultaneous many-by-many detection of direct protein-protein and protein-RNA interactions. Nat Commun. 2018 Sep 14;9(1):3747.
REF 33 Automated yeast two-hybrid screening for nuclear receptor-interacting proteins. Mol Cell Proteomics. 2005 Feb;4(2):205-13.
REF 34 A census of human soluble protein complexes. Cell. 2012 Aug 31;150(5):1068-81.
REF 35 Identification of human VPS37C, a component of endosomal sorting complex required for transport-I important for viral budding. J Biol Chem. 2005 Jan 7;280(1):628-36.
REF 36 Decoding the intrinsic mechanism that prohibits ALIX interaction with ESCRT and viral proteins. Biochem J. 2010 Dec 15;432(3):525-34.
REF 37 Human ESCRT-II complex and its role in human immunodeficiency virus type 1 release. J Virol. 2006 Oct;80(19):9465-80.

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