Target Binding Site Detail
| Target General Information | Top | ||||
|---|---|---|---|---|---|
| Target ID | T64553 | Target Info | |||
| Target Name | Tyrosine-protein kinase ZAP-70 (ZAP-70) | ||||
| Synonyms | Syk-related tyrosine kinase; SRK; 70 kDa zeta-chain associated protein | ||||
| Target Type | Patented-recorded Target | ||||
| Gene Name | ZAP70 | ||||
| Biochemical Class | Kinase | ||||
| UniProt ID | |||||
| Ligand General Information | Top | ||||
|---|---|---|---|---|---|
| Ligand Name | AMP-PNP | Ligand Info | |||
| Canonical SMILES | C1=NC(=C2C(=N1)N(C=N2)C3C(C(C(O3)COP(=O)(O)OP(=O)(NP(=O)(O)O)O)O)O)N | ||||
| InChI | 1S/C10H17N6O12P3/c11-8-5-9(13-2-12-8)16(3-14-5)10-7(18)6(17)4(27-10)1-26-31(24,25)28-30(22,23)15-29(19,20)21/h2-4,6-7,10,17-18H,1H2,(H,24,25)(H2,11,12,13)(H4,15,19,20,21,22,23)/t4-,6-,7-,10-/m1/s1 | ||||
| InChIKey | PVKSNHVPLWYQGJ-KQYNXXCUSA-N | ||||
| PubChem Compound ID | 33113 | ||||
| Drug Binding Sites of Target | Top | |||||
|---|---|---|---|---|---|---|
| PDB ID: 4K2R Structural basis for activation of ZAP-70 by phosphorylation of the SH2-kinase linker | ||||||
| Method | X-ray diffraction | Resolution | 3.00 Å | Mutation | Yes | [1] |
| PDB Sequence |
PDPAAHLPFF
11 YGSISRAEAE21 EHLKLAGMAD31 GLFLLRQCLR41 SLGGYVLSLV51 HDVRFHHFPI 61 ERQLNGTYAI71 AGGKAHCGPA81 ELCEFYSRDP91 DGLPCNLRKP101 CNRPSGLEPQ 111 PGVFDCLRDA121 MVRDYVRQTW131 KLEGEALEQA141 IISQAPQVEK151 LIATTAHERM 161 PWYHSSLTRE171 EAERKLYSGA181 QTDGKFLLRP191 RKEQGTYALS201 LIYGKTVYHY 211 LISQDKAGKY221 CIPEGTKFDT231 LWQLVEYLKL241 KADGLIYCLK251 EACPNSDKPR 306 PMPMDTSVYE316 SPYSDPEELK326 DKKLFLKRDN336 LLIADIELGC346 GNFGSVRQGV 356 YRMRKKQIDV366 AIKVLKQGTE376 KADTEEMMRE386 AQIMHQLDNP396 YIVRLIGVCQ 406 AEALMLVMEM416 AGGGPLHKFL426 VGKREEIPVS436 NVAELLHQVS446 MGMKYLEEKN 456 FVHRNLAARN466 VLLVNRHYAK476 ISDFGLSWPL503 KWYAPECINF513 RKFSSRSDVW 523 SYGVTMWEAL533 SYGQKPYKKM543 KGPEVMAFIE553 QGKRMECPPE563 CPPELYALMS 573 DCWIYKWEDR583 PDFLTVEQRM593 RACYYSLASK603 VEGGSGLEVL613 F |
|||||
|
|
LEU344
3.518
GLY345
3.951
CYS346
4.078
GLY347
3.652
ASN348
3.756
PHE349
3.692
GLY350
4.946
VAL352
3.430
ALA367
3.274
LYS369
2.826
VAL399
4.122
MET414
3.724
GLU415
2.957
|
|||||
| PDB ID: 2OZO Autoinhibited intact human ZAP-70 | ||||||
| Method | X-ray diffraction | Resolution | 2.60 Å | Mutation | Yes | [2] |
| PDB Sequence |
PDPAAHLPFF
11 YGSISRAEAE21 EHLKLAGMAD31 GLFLLRQCLR41 SLGGYVLSLV51 HDVRFHHFPI 61 ERQLNGTYAI71 AGGKAHCGPA81 ELCEFYSRDP91 DGLPCNLRKP101 CNRPSGLEPQ 111 PGVFDCLRDA121 MVRDYVRQTW131 KLEGEALEQA141 IISQAPQVEK151 LIATTAHERM 161 PWYHSSLTRE171 EAERKLYSGA181 QTDGKFLLRP191 RKEQGTYALS201 LIYGKTVYHY 211 LISQDKAGKY221 CIPEGTKFDT231 LWQLVEYLKL241 KADGLIYCLK251 EACPNMDTSV 314 FESPFSDPEE324 LKDKKLFLKR334 DNLLIADIEL344 GCGNFGSVRQ354 GVYRMRKKQI 364 DVAIKVLKQG374 TEKADTEEMM384 REAQIMHQLD394 NPYIVRLIGV404 CQAEALMLVM 414 EMAGGGPLHK424 FLVGKREEIP434 VSNVAELLHQ444 VSMGMKYLEE454 KNFVHRNLAA 464 RNVLLVNRHY474 AKISDFGLSK484 ALGPLKWYAP508 ECINFRKFSS518 RSDVWSYGVT 528 MWEALSYGQK538 PYKKMKGPEV548 MAFIEQGKRM558 ECPPECPPEL568 YALMSDCWIY 578 KWEDRPDFLT588 VEQRMRACYY598 SLASKVEGGS608 ALEVA
|
|||||
|
|
LEU344
3.402
GLY345
4.302
CYS346
3.629
GLY347
3.282
ASN348
3.087
PHE349
3.524
GLY350
4.218
VAL352
3.567
ALA367
3.315
LYS369
3.180
VAL399
4.035
MET414
3.900
GLU415
3.000
|
|||||
| References | Top | ||||
|---|---|---|---|---|---|
| REF 1 | Structural basis for activation of ZAP-70 by phosphorylation of the SH2-kinase linker. Mol Cell Biol. 2013 Jun;33(11):2188-201. | ||||
| REF 2 | Structural basis for the inhibition of tyrosine kinase activity of ZAP-70. Cell. 2007 May 18;129(4):735-46. | ||||
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