Target Binding Site Detail
Target General Information | Top | ||||
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Target ID | T54582 | Target Info | |||
Target Name | Nicotinamide phosphoribosyltransferase (NAMPT) | ||||
Synonyms | Visfatin; PreBcell colonyenhancing factor 1; PreB cellenhancing factor; Pre-B-cell colony-enhancing factor 1; Pre-B cell-enhancing factor; PBEF1; PBEF; Nampt; NAmPRTase | ||||
Target Type | Clinical trial Target | ||||
Gene Name | NAMPT | ||||
Biochemical Class | Glycosyltransferases | ||||
UniProt ID |
Ligand General Information | Top | ||||
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Ligand Name | Alpha-Phosphoribosylpyrophosphoric Acid | Ligand Info | |||
Canonical SMILES | C(C1C(C(C(O1)OP(=O)(O)OP(=O)(O)O)O)O)OP(=O)(O)O | ||||
InChI | 1S/C5H13O14P3/c6-3-2(1-16-20(8,9)10)17-5(4(3)7)18-22(14,15)19-21(11,12)13/h2-7H,1H2,(H,14,15)(H2,8,9,10)(H2,11,12,13)/t2-,3-,4-,5-/m1/s1 | ||||
InChIKey | PQGCEDQWHSBAJP-TXICZTDVSA-N | ||||
PubChem Compound ID | 7339 |
Drug Binding Sites of Target | Top | |||||
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PDB ID: 3DKJ Crystal structure of human NAMPT complexed with benzamide and phosphoribosyl pyrophosphate | ||||||
Method | X-ray diffraction | Resolution | 2.00 Å | Mutation | No | [1] |
PDB Sequence |
FNILLATDSY
18 KVTHYKQYPP28 NTSKVYSYFE38 CREYEETVFY60 GLQYILNKYL70 KGKVVTKEKI 80 QEAKDVYKEH90 FQDDVFNEKG100 WNYILEKYDG110 HLPIEIKAVP120 EGFVIPRGNV 130 LFTVENTDPE140 CYWLTNWIET150 ILVQSWYPIT160 VATNSREQKK170 ILAKYLLETS 180 GNLDGLEYKL190 HDFGYRGVSS200 QETAGIGASA210 HLVNFKGTDT220 VAGLALIKKY 230 YGTKDPVPGY240 SVPAAEHSTI250 TAWGKDHEKD260 AFEHIVTQFS270 SVPVSVVSDS 280 YDIYNACEKI290 WGEDLRHLIV300 SRSTQAPLII310 RPDSGNPLDT320 VLKVLEILGK 330 KFPVTENSKG340 YKLLPPYLRV350 IQGDGVDINT360 LQEIVEGMKQ370 KMWSIENIAF 380 GSGGGLLQKL390 TRDLLNCSFK400 CSYVVTNGLG410 INVFKDPVAD420 PNKRSKKGRL 430 SLHRTPAGNF440 VTLEEGKGDL450 EEYGQDLLHT460 VFKNGKVTKS470 YSFDEIRKNA 480 QLNI
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PDB ID: 2E5C Crystal structure of Human NMPRTase complexed with 5'-phosphoribosyl-1'-pyrophosphate | ||||||
Method | X-ray diffraction | Resolution | 2.20 Å | Mutation | No | [2] |
PDB Sequence |
EFNILLATDS
17 YKVTHYKQYP27 PNTSKVYSYF37 ECREKKYEET57 VFYGLQYILN67 KYLKGKVVTK 77 EKIQEAKDVY87 KEHFQDDVFN97 EKGWNYILEK107 YDGHLPIEIK117 AVPEGFVIPR 127 GNVLFTVENT137 DPECYWLTNW147 IETILVQSWY157 PITVATNSRE167 QKKILAKYLL 177 ETSGNLDGLE187 YKLHDFGYRG197 VSSQETAGIG207 ASAHLVNFKG217 TDTVAGLALI 227 KKYYGTKDPV237 PGYSVPAAEH247 STITAWGKDH257 EKDAFEHIVT267 QFSSVPVSVV 277 SDSYDIYNAC287 EKIWGEDLRH297 LIVSRSTQAP307 LIIRPDSGNP317 LDTVLKVLEI 327 LGKKFPVTEN337 SKGYKLLPPY347 LRVIQGDGVD357 INTLQEIVEG367 MKQKMWSIEN 377 IAFGSGGGLL387 QKLTRDLLNC397 SFKCSYVVTN407 GLGINVFKDP417 VADPNKRSKK 427 GRLSLHRTPA437 GNFVTLEEGK447 GDLEEYGQDL457 LHTVFKNGKV467 TKSYSFDEIR 477 KNAQLN
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PDB ID: 6TA0 Human NAMPT in complex with nicotinic acid and phosphoribosyl pyrophosphate | ||||||
Method | X-ray diffraction | Resolution | 1.58 Å | Mutation | No | [3] |
PDB Sequence |
EFNILLATDS
17 YKVTHYKQYP27 PNTSKVYSYF37 ECREKKYEET57 VFYGLQYILN67 KYLKGKVVTK 77 EKIQEAKDVY87 KEHFQDDVFN97 EKGWNYILEK107 YDGHLPIEIK117 AVPEGFVIPR 127 GNVLFTVENT137 DPECYWLTNW147 IETILVQSWY157 PITVATNSRE167 QKKILAKYLL 177 ETSGNLDGLE187 YKLHDFGYRG197 VSSQETAGIG207 ASAHLVNFKG217 TDTVAGLALI 227 KKYYGTKDPV237 PGYSVPAAEH247 STITAWGKDH257 EKDAFEHIVT267 QFSSVPVSVV 277 SDSYDIYNAC287 EKIWGEDLRH297 LIVSRSTQAP307 LIIRPDSGNP317 LDTVLKVLEI 327 LGKKFPVTEN337 SKGYKLLPPY347 LRVIQGDGVD357 INTLQEIVEG367 MKQKMWSIEN 377 IAFGSGGGLL387 QKLTRDLLNC397 SFKCSYVVTN407 GLGINVFKDP417 VADPNKRSKK 427 GRLSLHRTPA437 GNFVTLEEGK447 GDLEEYGQDL457 LHTVFKNGKV467 TKSYSFDEIR 477 KNAQLNI
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TYR18
4.000
ARG40
2.502
GLU149
4.407
PHE193
2.847
GLY194
4.856
ARG196
2.089
GLY197
4.221
HIS247
3.901
VAL277
4.960
ARG311
2.192
ASP313
1.907
GLY353
2.540
ASP354
2.665
GLY355
4.023
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References | Top | ||||
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REF 1 | A phosphoenzyme mimic, overlapping catalytic sites and reaction coordinate motion for human NAMPT. Proc Natl Acad Sci U S A. 2009 Aug 18;106(33):13748-53. | ||||
REF 2 | Structure and reaction mechanism of human nicotinamide phosphoribosyltransferase. J Biochem. 2010 Jan;147(1):95-107. | ||||
REF 3 | Identification of structural determinants of NAMPT activity and substrate selectivity |
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