Binding Site Information of Target
| Target General Information | Top | ||||
|---|---|---|---|---|---|
| Target ID | T63083 | Target Info | |||
| Target Name | DNA repair protein RAD51 homolog 1 (RAD51) | ||||
| Synonyms | hRAD51; RECA; RAD51A; RAD51 homolog A; HsRAD51 | ||||
| Target Type | Clinical trial Target | ||||
| Gene Name | RAD51 | ||||
| UniProt ID | |||||
| Drug Binding Sites of Target | Top | |||||
|---|---|---|---|---|---|---|
| Ligand Name: Adenosine triphosphate | Ligand Info | |||||
| Structure Description | Crystal structure of a human RAD51-ATP filament. | PDB:5NWL | ||||
| Method | X-ray diffraction | Resolution | 3.93 Å | Mutation | No | [1] |
| PDB Sequence |
GPQPISRLEQ
30 CGINANDVKK40 LEEAGFHTVE50 AVAYAPKKEL60 INIKGISEAK70 ADKILAEAAK 80 LVPMGFTTAT90 EFHQRRSEII100 QITTGSKELD110 KLLQGGIETG120 SITEMFGEFR 130 TGKTQICHTL140 AVTCQLPIDR150 GGGEGKAMYI160 DTEGTFRPER170 LLAVAERYGL 180 SGSDVLDNVA190 YARAFNTDHQ200 TQLLYQASAM210 MVESRYALLI220 VDSATALYRT 230 DYSGRGELSA240 RQMHLARFLR250 MLLRLADEFG260 VAVVITNQVV270 GNIIAHASTT 298 RLYLRKGRGE308 TRICKIYDSP318 CLPEAEAMFA328 INADGVGDAK338 D |
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| Ligand Name: AMP-PNP | Ligand Info | |||||
| Structure Description | Human RAD51 post-synaptic complexes mutant (V273P, D274G) | PDB:7C9A | ||||
| Method | Electron microscopy | Resolution | 3.43 Å | Mutation | Yes | [2] |
| PDB Sequence |
PQPISRLEQC
31 GINANDVKKL41 EEAGFHTVEA51 VAYAPKKELI61 NIKGISEAKA71 DKILAEAAKL 81 VPMGFTTATE91 FHQRRSEIIQ101 ITTGSKELDK111 LLQGGIETGS121 ITEMFGEFRT 131 GKTQICHTLA141 VTCQLPIDRG151 GGEGKAMYID161 TEGTFRPERL171 LAVAERYGLS 181 GSDVLDNVAY191 ARAFNTDHQT201 QLLYQASAMM211 VESRYALLIV221 DSATALYRTD 231 YSGRGELSAR241 QMHLARFLRM251 LLRLADEFGV261 AVVITNQVVA271 QPGGAADPKK 285 PIGGNIIAHA295 STTRLYLRKG305 RGETRICKIY315 DSPCLPEAEA325 MFAINADGVG 335 D
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PHE129
2.838
ARG130
3.650
THR131
3.353
GLY132
3.171
LYS133
2.645
THR134
2.675
GLN135
3.811
GLU163
3.897
ARG170
3.320
ALA293
3.782
HIS294
3.405
SER296
4.088
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| Click to View More Binding Site Information of This Target and Ligand Pair | ||||||
| Ligand Name: 3-(benzylsulfamoyl)-4-bromo-N-(4-bromophenyl)benzamide | Ligand Info | |||||
| Structure Description | human RAD51 presynaptic complex | PDB:7EJC | ||||
| Method | Electron microscopy | Resolution | 2.97 Å | Mutation | Yes | [3] |
| PDB Sequence |
PQPISRLEQC
31 GINANDVKKL41 EEAGFHTVEA51 VAYAPKKELI61 NIKGISEAKA71 DKILAEAAKL 81 VPMGFTTATE91 FHQRRSEIIQ101 ITTGSKELDK111 LLQGGIETGS121 ITEMFGEFRT 131 GKTQICHTLA141 VTCQLPIDRG151 GGEGKAMYID161 TEGTFRPERL171 LAVAERYGLS 181 GSDVLDNVAY191 ARAFNTDHQT201 QLLYQASAMM211 VESRYALLIV221 DSATALYRTD 231 YSGRGELSAR241 QMHLARFLRM251 LLRLADEFGV261 AVVITNQVVA271 QVDGPKKPIG 288 GNIIAHASTT298 RLYLRKGRGE308 TRICQIYDSP318 CLPEAEAMFA328 INADGVGDAK 338
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Click to Show 3D Structure of This Binding Site
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| References | Top | ||||
|---|---|---|---|---|---|
| REF 1 | Two distinct conformational states define the interaction of human RAD51-ATP with single-stranded DNA. EMBO J. 2018 Apr 3;37(7):e98162. | ||||
| REF 2 | Identification of fidelity-governing factors in human recombinases DMC1 and RAD51 from cryo-EM structures. Nat Commun. 2021 Jan 14;12(1):115. | ||||
| REF 3 | Mechanisms of distinctive mismatch tolerance between Rad51 and Dmc1 in homologous recombination. Nucleic Acids Res. 2021 Dec 16;49(22):13135-13149. | ||||
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