Binding Site Information of Target
| Target General Information | Top | ||||
|---|---|---|---|---|---|
| Target ID | T25200 | Target Info | |||
| Target Name | Ribonuclease L (RNASEL) | ||||
| Synonyms | Ribonuclease 4; RNase L; RNS4; 25Adependent ribonuclease; 25Adependent RNase; 2-5A-dependent ribonuclease; 2-5A-dependent RNase | ||||
| Target Type | Literature-reported Target | ||||
| Gene Name | RNASEL | ||||
| Biochemical Class | Endoribonucleases | ||||
| UniProt ID | |||||
| Drug Binding Sites of Target | Top | |||||
|---|---|---|---|---|---|---|
| Ligand Name: L-betagamma-meATP | Ligand Info | |||||
| Structure Description | Complete human RNase L in complex with 2-5A (5'-ppp heptamer), AMPPCP and RNA substrate. | PDB:4OAV | ||||
| Method | X-ray diffraction | Resolution | 2.10 Å | Mutation | Yes | [1] |
| PDB Sequence |
EDNHLLIKAV
33 QNEDVDLVQQ43 LLEGGANVNF53 QEEEGGWTPL63 HNAVQMSRED73 IVELLLRHGA 83 DPVLRKKNGA93 TPFILAAIAG103 SVKLLKLFLS113 KGADVNECDF123 YGFTAFMEAA 133 VYGKVKALKF143 LYKRGANVNL153 RRKTKEDQER163 LRKGGATALM173 DAAEKGHVEV 183 LKILLDEMGA193 DVNACDNMGR203 NALIHALLSS213 DDSDVEAITH223 LLLDHGADVN 233 VRGERGKTPL243 ILAVEKKHLG253 LVQRLLEQEH263 IEINDTDSDG273 KTALLLAVEL 283 KLKKIAELLC293 KRGASTDCGD303 LVMTARRNYD313 HSLVKVLLSH323 GAKEWKPQSS 341 HWGAALKDLH351 RIYRPMIGKL361 KFFIDEKYKI371 ADTSEGGIYL381 GFYEKQEVAV 391 KTFCEGSPRA401 QREVSCLQSS411 RENSHLVTFY421 GSESHRGHLF431 VCVTLCEQTL 441 EACLDVEEDE459 FARNVLSSIF469 KAVQELHLSC479 GYTHQDLQPQ489 NILIDSKKAA 499 HLADFDKSIK509 WAGDPQEVKR519 DLEDLGRLVL529 YVVKKGSISF539 EDLKAQSNEE 549 VVQLSPDEET559 KDLIHRLFHP569 GEHVRDCLSD579 LLGHPFFWTW589 ESRYRTLRNV 599 GNESDIKTRK609 SESEILRLLQ619 PGPSEHSKSF629 DKWTTKINEC639 VMKKMNKFYE 649 KRGNFYQNTV659 GDLLKFIRNL669 GENIDKMKLK684 IGDPSLYFQK694 TFPDLVIYVY 704 TKLQNTEYRK714 HFP
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TRP343
4.567
ILE371
3.629
ALA372
3.303
ASP373
4.865
THR374
2.988
SER375
2.218
ILE379
3.372
ALA390
3.263
LYS392
2.853
ARG400
2.924
GLU404
4.913
VAL418
4.383
VAL434
3.370
|
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| Ligand Name: adenosine diphosphate | Ligand Info | |||||
| Structure Description | Complete human RNase L in complex with biological activators. | PDB:4OAU | ||||
| Method | X-ray diffraction | Resolution | 2.60 Å | Mutation | Yes | [1] |
| PDB Sequence |
DNHLLIKAVQ
34 NEDVDLVQQL44 LEGGANVNFQ54 EEEGGWTPLH64 NAVQMSREDI74 VELLLRHGAD 84 PVLRKKNGAT94 PFILAAIAGS104 VKLLKLFLSK114 GADVNECDFY124 GFTAFMEAAV 134 YGKVKALKFL144 YKRGANVNLR154 RKTKEDQERL164 RKGGATALMD174 AAEKGHVEVL 184 KILLDEMGAD194 VNACDNMGRN204 ALIHALLSSD214 DSDVEAITHL224 LLDHGADVNV 234 RGERGKTPLI244 LAVEKKHLGL254 VQRLLEQEHI264 EINDTDSDGK274 TALLLAVELK 284 LKKIAELLCK294 RGASTDCGDL304 VMTARRNYDH314 SLVKVLLSHG324 AKEDFHPPAE 334 DWKPQSSHWG344 AALKDLHRIY354 RPMIGKLKFF364 IDEKYKIADT374 SEGGIYLGFY 384 EKQEVAVKTF394 CEGSPRAQRE404 VSCLQSSREN414 SHLVTFYGSE424 SHRGHLFVCV 434 TLCEQTLEAC444 LDVHRGEDVE454 NEEDEFARNV464 LSSIFKAVQE474 LHLSCGYTHQ 484 DLQPQNILID494 SKKAAHLADF504 DKSIKWAGDP514 QEVKRDLEDL524 GRLVLYVVKK 534 GSISFEDLKA544 QSNEEVVQLS554 PDEETKDLIH564 RLFHPGEHVR574 DCLSDLLGHP 584 FFWTWESRYR594 TLRNVGNESD604 IKTRKSESEI614 LRLLQPEHSK627 SFDKWTTKIN 637 ECVMKKMNKF647 YEKRGNFYQN657 TVGDLLKFIR667 NLGENIDEEK677 HKKMKLKIGD 687 PSLYFQKTFP697 DLVIYVYTKL707 QNTEYRKHFP717 QT
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TRP343
4.824
ILE371
3.177
ALA372
3.315
ASP373
4.887
THR374
2.298
SER375
4.209
ILE379
3.147
ALA390
3.347
LYS392
2.993
ARG400
4.344
GLU404
4.882
VAL418
4.571
VAL434
3.405
|
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| Ligand Name: 5'-O-Monophosphoryladenylyl(2'->5')adenylyl(2'->5')adenosine | Ligand Info | |||||
| Structure Description | Crystal structure of ribonuclease | PDB:1WDY | ||||
| Method | X-ray diffraction | Resolution | 1.80 Å | Mutation | Yes | [2] |
| PDB Sequence |
AAVEDNHLLI
30 KAVQNEDVDL40 VQQLLEGGAN50 VNFQEEEGGW60 TPLHNAVQMS70 REDIVELLLR 80 HGADPVLRKK90 NGATPFLLAA100 IAGSVKLLKL110 FLSKGADVNE120 CDFYGFTAFM 130 EAAVYGKVKA140 LKFLYKRGAN150 VNLRRKTKED160 QERLRKGGAT170 ALMDAAEKGH 180 VEVLKILLDE190 MGADVNACDN200 MGRNALIHAL210 LSSDDSDVEA220 ITHLLLDHGA 230 DVNVRGERGK240 TPLILAVEKK250 HLGLVQRLLE260 QEHIEINDTD270 SDGKTALLLA 280 VELKLKKIAE290 LLCKRGASTD300 CGDLV
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Click to Show 3D Structure of This Binding Site
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| Click to View More Binding Site Information of This Target and Ligand Pair | ||||||
| References | Top | ||||
|---|---|---|---|---|---|
| REF 1 | Structure of human RNase L reveals the basis for regulated RNA decay in the IFN response. Science. 2014 Mar 14;343(6176):1244-8. | ||||
| REF 2 | Structural basis for recognition of 2',5'-linked oligoadenylates by human ribonuclease L. EMBO J. 2004 Oct 13;23(20):3929-38. | ||||
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