Target Information
| Target General Information | Top | |||||
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| Target ID |
T08280
(Former ID: TTDR00635)
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| Target Name |
Trypanosoma Pyrophosphate-dependent phosphofructokinase (Trypano pfk)
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| Synonyms |
Pyrophosphate-dependent 6-phosphofructose-1-kinase; Pyrophosphate--fructose 6-phosphate 1-phosphotransferase alpha subunit; PPi-PFK; PFP-ALPHA; PFP; 6-phosphofructokinase, pyrophosphate dependent
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| Gene Name |
Trypano pfk
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| Target Type |
Literature-reported target
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[1] | ||||
| Function |
Regulatory subunit of pyrophosphate--fructose 6- phosphate 1-phosphotransferase.
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| BioChemical Class |
Kinase
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| UniProt ID | ||||||
| EC Number |
EC 2.7.1.11
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| Sequence |
MAVESRSRVTSKLVKAHRAMLNSVTQEDLKVDRLPGADYPNPSKKYSSRTEFRDKTDYIM
YNPRPRDEPSSENPVSVSPLLCELAAARSRIHFNPTETTIGIVTCGGICPGLNDVIRSIT LTGINVYNVKRVIGFRFGYWGLSKKGSQTAIELHRGRVTNIHHYGGTILGSSRGPQDPKE MVDTLERLGVNILFTVGGDGTQRGALVISQEAKRRGVDISVFGVPKTIDNDLSFSHRTFG FQTAVEKAVQAIRAAYAEAVSANYGVGVVKLMGRDSGFIAAQAAVASAQANICLVPENPI SEQEVMSLLERRFCHSRSCVIIVAEGFGQDWGRGSGGYDASGNKKLIDIGVILTEKVKAF LKANKSRYPDSTVKYIDPSYMIRACPPSANDALFCATLATLAVHEAMAGATGCIIAMRHN NYILVPIKVATSVRRVLDLRGQLWRQVREITVDLGSDVRLARKLEIRRELEAINRNRDRL HEELAKL Click to Show/Hide
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| 3D Structure | Click to Show 3D Structure of This Target | PDB | ||||
| Drug Binding Sites of Target | Top | |||||
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| Ligand Name: Adenosine triphosphate | Ligand Info | |||||
| Structure Description | Crystal Structure of ATP-Bound Phosphofructokinase from Trypanosoma brucei | PDB:3F5M | ||||
| Method | X-ray diffraction | Resolution | 2.70 Å | Mutation | No | [2] |
| PDB Sequence |
TSKLVKAHRA
19 MLNSVTQEDL29 KVDRLPGADY39 PNPSKKKTDY58 IMYNPRPREN73 PVSVSPLLCE 83 LAAARSRIHF93 NPTETTIGIV103 TCGGICPGLN113 DVIRSITLTG123 INVYNVKRVI 133 GFRFGYWGLS143 KKGSQTAIEL153 HRGRVTNIHH163 YGGTILGSSR173 GPQDPKEMVD 183 TLERLGVNIL193 FTVGGDGTQR203 GALVISQEAK213 RRGVDISVFG223 VPKTIDNDLS 233 FSHRTFGFQT243 AVEKAVQAIR253 AAYAEAVSAN263 YGVGVVKLMG273 RDSGFIAAQA 283 AVASAQANIC293 LVPENPISEQ303 EVMSLLERRF313 CHSRSCVIIV323 AEGFGQDWGR 333 YDASGNKKLI347 DIGVILTEKV357 KAFLKANKSR367 YPDSTVKYID377 PSYMIRACPP 387 SANDALFCAT397 LATLAVHEAM407 AGATGCIIAM417 RHNNYILVPI427 KVATSVRRVL 437 DLRGQLWRQV447 REITVDLGSD457 VRLARKLEIR467 RELEAINRNR477 DRLHEELA |
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CYS105
3.843
GLY106
3.203
GLY107
2.722
TYR139
3.552
SER172
4.167
ARG173
2.709
GLY174
2.452
PRO175
2.843
GLY197
3.821
GLY198
2.663
ASP199
3.673
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| Ligand Name: Adenosine monophosphate | Ligand Info | |||||
| Structure Description | Structure of Trypanosome Brucei Phosphofructokinase in complex with AMP. | PDB:6SY7 | ||||
| Method | X-ray diffraction | Resolution | 2.75 Å | Mutation | No | [3] |
| PDB Sequence |
MLNSVTQEDL
29 KVDRLPGADY39 PNPSKKYSSR49 TEFRDKTDYI59 MYNPRPRDEP69 SSENPVSVSP 79 LLCELAAARS89 RIHFNPTETT99 IGIVTCGGIC109 PGLNDVIRSI119 TLTGINVYNV 129 KRVIGFRFGY139 WGLSKKGSQT149 AIELHRGRVT159 NIHHYGGTIL169 GSSRGPQDPK 179 EMVDTLERLG189 VNILFTVGGD199 GTQRGALVIS209 QEAKRRGVDI219 SVFGVPKTID 229 NDLSFSHRTF239 GFQTAVEKAV249 QAIRAAYAEA259 VSANYGVGVV269 KLMGRDSGFI 279 AAQAAVASAQ289 ANICLVPENP299 ISEQEVMSLL309 ERRFCHSRSC319 VIIVAEGFGQ 329 DWGLIDIGVI352 LTEKVKAFLK362 ANKSRYPDST372 VKYIDPSYMI382 RACPPSANDA 392 LFCATLATLA402 VHEAMAGATG412 CIIAMRHNNY422 ILVPIKVATS432 VRRVLDLRGQ 442 LWRQVREITV452 DLGSDVRLAR462 KLEIRRELEA472 INRNRDRLHE482 ELAKL |
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| Click to View More Binding Site Information of This Target with Different Ligands | ||||||
| Different Human System Profiles of Target | Top |
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Human Similarity Proteins
of target is determined by comparing the sequence similarity of all human proteins with the target based on BLAST. The similarity proteins for a target are defined as the proteins with E-value < 0.005 and outside the protein families of the target.
A target that has fewer human similarity proteins outside its family is commonly regarded to possess a greater capacity to avoid undesired interactions and thus increase the possibility of finding successful drugs
(Brief Bioinform, 21: 649-662, 2020).
Human Similarity Proteins
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There is no similarity protein (E value < 0.005) for this target
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| Chemical Structure based Activity Landscape of Target | Top |
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| Target Poor or Non Binders | Top | |||||
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| Target Poor or Non Binders | ||||||
| Target-Related Models and Studies | Top | |||||
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| Target Validation | ||||||
| References | Top | |||||
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| REF 1 | Antitrypanosomal alkaloids from Polyalthia suaveolens (Annonaceae): their effects on three selected glycolytic enzymes of Trypanosoma brucei. Bioorg Med Chem Lett. 2010 Jun 15;20(12):3495-8. | |||||
| REF 2 | The crystal structure of ATP-bound phosphofructokinase from Trypanosoma brucei reveals conformational transitions different from those of other phosphofructokinases. J Mol Biol. 2009 Feb 6;385(5):1519-33. | |||||
| REF 3 | Kinetic and structural studies of Trypanosoma and Leishmania phosphofructokinases show evolutionary divergence and identify AMP as a switch regulating glycolysis versus gluconeogenesis. FEBS J. 2020 Jul;287(13):2847-2861. | |||||
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