Target Binding Site Detail
| Target General Information | Top | ||||
|---|---|---|---|---|---|
| Target ID | T86773 | Target Info | |||
| Target Name | ATP-dependent protease Lon (LONP1) | ||||
| Synonyms | Serine protease 15; Mitochondrial ATP-dependent protease Lon; Lon protease-like protein; LONP1; LONP; LONHs | ||||
| Target Type | Literature-reported Target | ||||
| Gene Name | LONP1 | ||||
| Biochemical Class | Peptidase | ||||
| UniProt ID | |||||
| Ligand General Information | Top | ||||
|---|---|---|---|---|---|
| Ligand Name | adenosine diphosphate | Ligand Info | |||
| Canonical SMILES | C1=NC(=C2C(=N1)N(C=N2)C3C(C(C(O3)COP(=O)(O)OP(=O)(O)O)O)O)N | ||||
| InChI | 1S/C10H15N5O10P2/c11-8-5-9(13-2-12-8)15(3-14-5)10-7(17)6(16)4(24-10)1-23-27(21,22)25-26(18,19)20/h2-4,6-7,10,16-17H,1H2,(H,21,22)(H2,11,12,13)(H2,18,19,20)/t4-,6-,7-,10-/m1/s1 | ||||
| InChIKey | XTWYTFMLZFPYCI-KQYNXXCUSA-N | ||||
| PubChem Compound ID | 6022 | ||||
| Drug Binding Sites of Target | Top | |||||
|---|---|---|---|---|---|---|
| PDB ID: 7OXO human LonP1, R-state, incubated in AMPPCP | ||||||
| Method | Electron microscopy | Resolution | 3.90 Å | Mutation | No | [1] |
| PDB Sequence |
HLPLIAITRN
132 PVFPRFIKII142 EVKNKKLVEL152 LRRKVRLAQP162 YVGVFLKRDD172 SNESDVVESL 182 DEIYHTGTFA192 QIHEMQDLGD202 KLRMIVMGHR212 RVHISRQLEM272 VEVENVVHED 282 FQVTEEVKAL292 TAEIVKTIRD302 IIALNPLYRE312 SVLQMMQAGQ322 RVVDNPIYLS 332 DMGAALTGAE342 SHELQDVLEE352 TNIPKRLYKA362 LSLLKKEFEL372 SKLQQRLGRE 382 VEEKIKQTHR392 KYLLQEQLKI402 IKKELGLEKD412 DKDAIEEKFR422 ERLKELVVPK 432 HVMDVVDEEL442 SKLGLLDNHS452 SEFNVTRNYL462 DWLTSIPWGK472 YSNENLDLAR 482 AQAVLEEDHY492 GMEDVKKRIL502 EFIAVSQLRG512 STQGKILCFY522 GPPGVGKTSI 532 ARSIARALNR542 EYFRFSVGGM552 TDVAEIKGHR562 RTYVGAMPGK572 IIQCLKKTKT 582 ENPLILIDEV592 DKIGRGYQGD602 PSSALLELLD612 PEQNANFLDH622 YLDVPVDLSK 632 VLFICTANVT642 DTIPEPLRDR652 MEMINVSGYV662 AQEKLAIAER672 YLVPQARALC 682 GLDESKAKLS692 SDVLTLLIKQ702 YCRESGVRNL712 QKQVEKVLRK722 SAYKIVSGEA 732 ESVEVTPENL742 QDFVGKPVFT752 VERMYDVTPP762 GVVMGLAWTA772 MGGSTLFVET 782 SLRRPQDKDA792 KGDKDGSLEV802 TGQLGEVMKE812 SARIAYTFAR822 AFLMQHAPAN 832 DYLVTSHIHL842 HVPEGATPKD852 GPSAGCTIVT862 ALLSLAMGRP872 VRQNLAMTGE 882 VSLTGKILPV892 GGIKEKTIAA902 KRAGVTCIVL912 PAENKKDFYD922 LAAFITEGLE 932 VHFVEHYREI942 FDIAFP
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ASP490
2.761
HIS491
2.495
TYR492
2.655
GLY493
4.623
MET494
4.234
PRO525
4.490
GLY526
1.828
VAL527
3.485
GLY528
2.430
LYS529
2.920
THR530
2.146
|
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| PDB ID: 7P0B Human mitochondrial Lon protease without substrate | ||||||
| Method | Electron microscopy | Resolution | 4.11 Å | Mutation | No | [2] |
| PDB Sequence |
EKDDKDAIEE
419 KFRERLKELV429 VPKHVMDVVD439 EELSKLGLLD449 NHSSEFNVTR459 NYLDWLTSIP 469 WGKYSNENLD479 LARAQAVLEE489 DHYGMEDVKK499 RILEFIAVSQ509 LRGSTQGKIL 519 CFYGPPGVGK529 TSIARSIARA539 LNREYFRFSV549 GGMTDVAEIK559 GHRWTYVGAM 569 PGKIIQCLKK579 TKTENPLILI589 DEVDMIGRGY599 QGDPSSALLE609 LLDPEQNANF 619 LDHYLDVPVD629 LSKVLFICTA639 NVTDTIPEPL649 RDRMEMINVS659 GYVAQEKLAI 669 AERYLVPQAR679 ALCGLDESKA689 KLSSDVLTLL699 IKQYCRESGV709 RNLQKQVEKV 719 LRKSAYKIVS729 GEAESVEVTP739 ENLQDFVGKP749 VFTVERMYDV759 TPPGVVMGLA 769 WTAMGGSTLF779 VETSLRRKDG798 SLEVTGQLGE808 VMKESARIAY818 TFARAFLMQH 828 APANDYLVTS838 HIHLHVPEGA848 TPKDGPSAGC858 TIVTALLSLA868 MGRPVRQNLA 878 MTGEVSLTGK888 ILPVGGIKEK898 TIAAKRAGVT908 CIVLPAENKK918 DFYDLAAFIT 928 EGLEVHFVEH938 YREIFDIAFP948 D
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| PDB ID: 7NGL R-state of wild type human mitochondrial LONP1 protease bound to endogenous ADP | ||||||
| Method | Electron microscopy | Resolution | 3.80 Å | Mutation | No | [1] |
| PDB Sequence |
HLPLIAITRN
132 PVFPRFIKII142 EVKNKKLVEL152 LRRKVRLAQP162 YVGVFLKRDD172 SNESDVVESL 182 DEIYHTGTFA192 QIHEMQDLGD202 KLRMIVMGHR212 RVHISRQLEM272 VEVENVVHED 282 FQVTEEVKAL292 TAEIVKTIRD302 IIALNPLYRE312 SVLQMMQAGQ322 RVVDNPIYLS 332 DMGAALTGAE342 SHELQDVLEE352 TNIPKRLYKA362 LSLLKKEFEL372 SKLQQRLGRE 382 VEEKIKQTHR392 KYLLQEQLKI402 IKKELGLEKD412 DKDAIEEKFR422 ERLKELVVPK 432 HVMDVVDEEL442 SKLGLLDNHS452 SEFNVTRNYL462 DWLTSIPWGK472 YSNENLDLAR 482 AQAVLEEDHY492 GMEDVKKRIL502 EFIAVSQLRG512 STQGKILCFY522 GPPGVGKTSI 532 ARSIARALNR542 EYFRFSVGGM552 TDVAEIKGHR562 RTYVGAMPGK572 IIQCLKKTKT 582 ENPLILIDEV592 DKIGRGYQGD602 PSSALLELLD612 PEQNANFLDH622 YLDVPVDLSK 632 VLFICTANVT642 DTIPEPLRDR652 MEMINVSGYV662 AQEKLAIAER672 YLVPQARALC 682 GLDESKAKLS692 SDVLTLLIKQ702 YCRESGVRNL712 QKQVEKVLRK722 SAYKIVSGEA 732 ESVEVTPENL742 QDFVGKPVFT752 VERMYDVTPP762 GVVMGLAWTA772 MGGSTLFVET 782 SLRRPQDKDA792 KGDKDGSLEV802 TGQLGEVMKE812 SARIAYTFAR822 AFLMQHAPAN 832 DYLVTSHIHL842 HVPEGATPKD852 GPSAGCTIVT862 ALLSLAMGRP872 VRQNLAMTGE 882 VSLTGKILPV892 GGIKEKTIAA902 KRAGVTCIVL912 PAENKKDFYD922 LAAFITEGLE 932 VHFVEHYREI942 FDIAFP
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ASP490
3.359
HIS491
2.807
TYR492
2.176
GLY493
4.512
MET494
3.743
PRO525
4.447
GLY526
2.009
VAL527
2.881
GLY528
2.422
LYS529
1.790
THR530
2.153
|
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| PDB ID: 7KSL Substrate-free human mitochondrial LONP1 | ||||||
| Method | Electron microscopy | Resolution | 3.50 Å | Mutation | No | [3] |
| PDB Sequence |
FRERLKELVV
430 PKHVMDVVDE440 ELSKLGLLDN450 HSSEFNVTRN460 YLDWLTSIPW470 GKYSNENLDL 480 ARAQAVLEED490 HYGMEDVKKR500 ILEFIAVSQL510 RGSTQGKILC520 FYGPPGVGKT 530 SIARSIARAL540 NREYFRFSVG550 GMTDVAEIGK572 IIQCLKKTKT582 ENPLILIDEV 592 DKIPSSALLE609 LLDPEQNANF619 LDHYLDVPVD629 LSKVLFICTA639 NVTDTIPEPL 649 RDRMEMINVS659 GYVAQEKLAI669 AERYLVPQAR679 ALCGLDESSS693 DVLTLLIKQY 703 CRESGVRNLQ713 KQVEKVLRKS723 AYKIVSGENL742 QDFVGKPVFT752 VERMYDVTPP 762 GVVMGLAWTA772 MGGSTLFVET782 SLRRPQDKDG798 SLEVTGQLGE808 VMKESARIAY 818 TFARAFLMQH828 APANDYLVTS838 HIHLHVPEGA848 TPKDGPSAGC858 TIVTALLSLA 868 MGRPVRQNLA878 MTGEVSLTGK888 ILPVGGIKEK898 TIAAKRAGVT908 CIVLPAENKK 918 DFYDLAAFIT928 EGLEVHFVEH938 YREIFDIAF
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Click to Show 3D Structure of This Binding Site
set background white;style ions nothing; color 8e8e8e;style chemicals nothing; select .ADP or .ADP2 or .ADP3 or :3ADP;style chemicals stick;color identity;select .C:490 or .C:491 or .C:492 or .C:494 or .C:524 or .C:525 or .C:526 or .C:527 or .C:528 or .C:529 or .C:530 or .C:531 or .C:638 or .C:661 or .C:669 or .C:673 or .C:709 or .C:713; color #f3c393; zoom selection;set surface opacity 0.5;set surface Van der Waals surface;set mode all
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| References | Top | ||||
|---|---|---|---|---|---|
| REF 1 | Catalytic cycling of human mitochondrial Lon protease. Structure. 2022 Sep 1;30(9):1254-1268.e7. | ||||
| REF 2 | A dual allosteric pathway drives human mitochondrial Lon | ||||
| REF 3 | Structures of the human LONP1 protease reveal regulatory steps involved in protease activation. Nat Commun. 2021 May 28;12(1):3239. | ||||
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