Target Information

Target General Information

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Target ID

T92208

Target Name

Serine/threonine-protein kinase Nek8 (NEK8)

Synonyms

Nima-related protein kinase 12a; NimA-related protein kinase 8; Never in mitosis A-related kinase 8; NEK12A; JCK

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Gene Name

NEK8

Target Type

Literature-reported target

[1]

Function

Required for renal tubular integrity. May regulate local cytoskeletal structure in kidney tubule epithelial cells. May regulate ciliary biogenesis through targeting of proteins to the cilia. Plays a role in organogenesis and is involved in the regulation of the Hippo signaling pathway.

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BioChemical Class

Protein kinase superfamily. NEK Ser/Thr protein kinase family

UniProt ID

NEK8_HUMAN

EC Number

EC 2.7.11.1

Sequence

MEKYERIRVVGRGAFGIVHLCLRKADQKLVIIKQIPVEQMTKEERQAAQNECQVLKLLNH
PNVIEYYENFLEDKALMIAMEYAPGGTLAEFIQKRCNSLLEEETILHFFVQILLALHHVH
THLILHRDLKTQNILLDKHRMVVKIGDFGISKILSSKSKAYTVVGTPCYISPELCEGKPY
NQKSDIWALGCVLYELASLKRAFEAANLPALVLKIMSGTFAPISDRYSPELRQLVLSLLS
LEPAQRPPLSHIMAQPLCIRALLNLHTDVGSVRMRRAEKSVAPSNTGSRTTSVRCRGIPR
GPVRPAIPPPLSSVYAWGGGLGTPLRLPMLNTEVVQVAAGRTQKAGVTRSGRLILWEAPP
LGAGGGSLLPGAVEQPQPQFISRFLEGQSGVTIKHVACGDFFTACLTDRGIIMTFGSGSN
GCLGHGSLTDISQPTIVEALLGYEMVQVACGASHVLALSTERELFAWGRGDSGRLGLGTR
ESHSCPQQVPMPPGQEAQRVVCGIDSSMILTVPGQALACGSNRFNKLGLDHLSLGEEPVP
HQQVEEALSFTLLGSAPLDQEPLLSIDLGTAHSAAVTASGDCYTFGSNQHGQLGTNTRRG
SRAPCKVQGLEGIKMAMVACGDAFTVAIGAESEVYSWGKGARGRLGRRDEDAGLPRPVQL
DETHPYTVTSVSCCHGNTLLAVRSVTDEPVPP

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3D Structure

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AlphaFold

Cell-based Target Expression Variations

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Cell-based Target Expression Variations

Cell-based Target Expression Variations Info

Different Human System Profiles of Target	Top
Human Similarity Proteins of target is determined by comparing the sequence similarity of all human proteins with the target based on BLAST. The similarity proteins for a target are defined as the proteins with E-value < 0.005 and outside the protein families of the target. A target that has fewer human similarity proteins outside its family is commonly regarded to possess a greater capacity to avoid undesired interactions and thus increase the possibility of finding successful drugs (Brief Bioinform, 21: 649-662, 2020). Human Similarity Proteins

Different Human System Profiles of Target

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Human Similarity Proteins of target is determined by comparing the sequence similarity of all human proteins with the target based on BLAST. The similarity proteins for a target are defined as the proteins with E-value < 0.005 and outside the protein families of the target.

A target that has fewer human similarity proteins outside its family is commonly regarded to possess a greater capacity to avoid undesired interactions and thus increase the possibility of finding successful drugs (Brief Bioinform, 21: 649-662, 2020).

Human Similarity Proteins

Protein Name	Pfam ID	Percentage of Identity (%)	E value
TGF-beta-activated kinase 1 (MAP3K7)	PF07714	31.621 (80/253)	8.14E-25
LIM domain kinase-2 (LIMK-2)	PF00412 ; PF00595 ; PF07714 More >>	28.519 (77/270)	2.24E-24
Mitogen-activated protein kinase kinase kinase 13 (MAP3K13)	PF07714	27.106 (74/273)	2.49E-24
Mitogen-activated protein kinase kinase kinase 12 (MAP3K12)	PF07714	27.106 (74/273)	6.16E-24
Mixed lineage kinase 3 (MAP3K11)	PF07714 ; PF14604	27.203 (71/261)	1.79E-23
LIM domain kinase-1 (LIMK-1)	PF00412 ; PF00595 ; PF07714 More >>	25.887 (73/282)	3.09E-22
Titin (TTN)		26.429 (74/280)	1.82E-21
Janus kinase 2 (JAK-2)	PF18379 ; PF18377 ; PF17887 ; PF07714 ; PF00017 More >>	31.034 (63/203)	1.15E-20
Tyrosine-protein kinase CSK (CSK)	PF07714 ; PF00017 ; PF00018 More >>	26.357 (68/258)	2.86E-20
TYK2 tyrosine kinase (TYK2)	PF18379 ; PF18377 ; PF17887 ; PF07714 More >>	31.527 (64/203)	5.69E-20
Janus kinase 3 (JAK-3)	PF18379 ; PF18377 ; PF17887 ; PF07714 More >>	32.338 (65/201)	1.65E-19
Serine/threonine-protein kinase A-Raf (ARAF)	PF00130 ; PF07714 ; PF02196 More >>	25.517 (74/290)	2.08E-19
Mixed lineage kinase 2 (MAP3K10)	PF07714 ; PF14604	26.054 (68/261)	2.66E-19
Serine/threonine-protein kinase B-raf (BRAF)	PF00130 ; PF07714 ; PF02196 More >>	25.714 (72/280)	3.93E-19
Tyrosine-protein kinase BMX (BMX)	PF00779 ; PF00169 ; PF07714 ; PF00017 More >>	26.459 (68/257)	8.19E-19
Megakaryocyte-associated tyrosine-protein kinase (MATK)	PF07714 ; PF00017 ; PF00018 More >>	28.112 (70/249)	1.22E-18
Focal adhesion kinase 1 (FAK)	PF00373 ; PF18038 ; PF03623 ; PF07714 More >>	24.688 (79/320)	1.65E-18
Ephrin type-A receptor 3 (EPHA3)	PF14575 ; PF01404 ; PF07699 ; PF00041 ; PF07714 ; PF07647 More >>	27.059 (69/255)	3.73E-18
T-cell-specific kinase (ITK)	PF00779 ; PF00169 ; PF07714 ; PF00017 ; PF00018 More >>	26.190 (66/252)	3.88E-18
Ephrin type-A receptor 5 (EPHA5)	PF14575 ; PF01404 ; PF00041 ; PF07714 ; PF00536 More >>	25.714 (72/280)	4.57E-18
Testis-specific kinase 1 (TESK1)	PF07714	24.806 (64/258)	4.60E-18
Tyrosine-protein kinase ABL2 (ABL2)	PF08919 ; PF07714 ; PF00017 ; PF00018 More >>	26.210 (65/248)	5.98E-18
Ephrin type-A receptor 7 (EPHA7)	PF14575 ; PF01404 ; PF07699 ; PF00041 ; PF07714 ; PF00536 More >>	26.772 (68/254)	5.99E-18
Janus kinase 1 (JAK-1)	PF18379 ; PF18377 ; PF17887 ; PF07714 More >>	30.244 (62/205)	1.28E-17
MLK-related kinase (MLTK)	PF07714 ; PF00536	26.904 (53/197)	1.35E-17
Proto-oncogene c-RAF (c-RAF)	PF00130 ; PF07714 ; PF02196 More >>	26.336 (69/262)	3.32E-17
B lymphocyte kinase (BLK)	PF07714 ; PF00017 ; PF00018 More >>	26.295 (66/251)	3.69E-17
Ephrin type-A receptor 1 (EPHA1)	PF14575 ; PF01404 ; PF07699 ; PF00041 ; PF07714 ; PF00536 More >>	24.000 (66/275)	4.33E-17
Focal adhesion kinase 2 (PTK2B)	PF00373 ; PF18038 ; PF03623 ; PF07714 More >>	23.175 (73/315)	6.60E-17
Tyrosine-protein kinase BRK (PTK6)	PF07714 ; PF00017 ; PF00018 More >>	25.806 (64/248)	7.86E-17
Tie-1 tyrosine kinase receptor (TIE1)	PF00041 ; PF00047 ; PF07714 More >>	25.094 (67/267)	1.09E-16
Testis-specific kinase 2 (TESK2)	PF07714	24.498 (61/249)	1.10E-16
Ephrin type-A receptor 2 (EPHA2)	PF14575 ; PF01404 ; PF00041 ; PF07714 ; PF00536 More >>	24.272 (75/309)	1.46E-16
BDNF/NT-3 growth factors receptor (TrkB)	PF07679 ; PF13855 ; PF16920 ; PF01462 ; PF07714 More >>	26.374 (72/273)	1.76E-16
Mixed lineage kinase 4 (MAP3K21)	PF07714 ; PF14604	27.982 (61/218)	3.97E-16
Ephrin type-A receptor 8 (EPHA8)	PF14575 ; PF01404 ; PF00041 ; PF07714 ; PF00536 More >>	25.114 (55/219)	4.04E-16
Tropomyosin-related kinase A (TrkA)	PF13855 ; PF16920 ; PF07714 ; PF18613 More >>	25.926 (70/270)	4.92E-16
Ephrin type-A receptor 4 (EPHA4)	PF14575 ; PF01404 ; PF00041 ; PF07714 ; PF07647 More >>	25.984 (66/254)	5.75E-16
Epithelial discoidin domain receptor 1 (DDR1)	PF00754 ; PF07714	26.644 (77/289)	7.26E-16
Fyn tyrosine protein kinase (FYN)	PF07714 ; PF00017 ; PF00018 More >>	23.438 (60/256)	7.30E-16
Proto-oncogene c-Src (SRC)	PF07714 ; PF00017 ; PF00018 More >>	25.806 (64/248)	1.17E-15
Tyrosine-protein kinase ABL1 (ABL)	PF08919 ; PF07714 ; PF00017 ; PF00018 More >>	25.654 (49/191)	1.73E-15
Neurotrophic tyrosine kinase ROR1 (ROR1)	PF01392 ; PF07679 ; PF00051 ; PF07714 More >>	24.719 (66/267)	2.50E-15
Proto-oncogene c-Fes (FES)	PF07714 ; PF00017	26.437 (69/261)	8.15E-15
Angiopoietin 1 receptor (TEK)	PF00041 ; PF10430 ; PF07714 More >>	27.273 (72/264)	8.65E-15
Tyrosine-protein kinase Fgr (FGR)	PF07714 ; PF00017 ; PF00018 More >>	24.609 (63/256)	9.28E-15
Kinase suppressor of Ras 2 (KSR2)	PF07714 ; PF13543 ; PF20406 More >>	23.970 (64/267)	1.14E-14
Tyrosine-protein kinase FRK (FRK)	PF07714 ; PF00017 ; PF00018 More >>	22.439 (46/205)	1.33E-14
Ephrin type-B receptor 2 (EPHB2)	PF14575 ; PF01404 ; PF07699 ; PF00041 ; PF07714 ; PF00536 More >>	24.219 (62/256)	1.35E-14
Tyrosine-protein kinase ZAP-70 (ZAP-70)	PF07714 ; PF00017	24.590 (60/244)	1.40E-14
Non-receptor tyrosine-protein kinase TNK1 (TNK1)	PF07714	27.336 (79/289)	2.32E-14
Hematopoietic cell kinase (HCK)	PF07714 ; PF00017 ; PF00018 More >>	23.810 (60/252)	2.33E-14
Tyrosine-protein kinase TXK (TXK)	PF07714 ; PF00017 ; PF00018 More >>	25.385 (66/260)	8.21E-14
Epidermal growth factor receptor (EGFR)	PF00757 ; PF14843 ; PF07714 ; PF01030 More >>	26.923 (56/208)	9.45E-14
Neurotrophic tyrosine kinase ROR2 (ROR2)	PF01392 ; PF07679 ; PF00051 ; PF07714 More >>	22.823 (76/333)	1.16E-13
Tyrosine-protein kinase Tec (PSCTK4)	PF00779 ; PF00169 ; PF07714 ; PF00017 ; PF00018 More >>	24.400 (61/250)	1.22E-13
Proto-oncogene c-Ret (RET)	PF00028 ; PF07714 ; PF17756 ; PF17812 ; PF17813 More >>	26.354 (73/277)	1.25E-13
Tyrosine-protein kinase BTK (ATK)	PF00779 ; PF00169 ; PF07714 ; PF00017 ; PF00018 More >>	27.136 (54/199)	1.45E-13
Receptor-interacting protein 1 (RIPK1)	PF00531 ; PF07714 ; PF12721 More >>	24.332 (82/337)	1.47E-13
Tyrosine-protein kinase Lyn (JTK8)	PF07714 ; PF00017 ; PF00018 More >>	23.320 (59/253)	2.12E-13
Erbb3 tyrosine kinase receptor (Erbb-3)	PF00757 ; PF14843 ; PF07714 ; PF01030 More >>	23.500 (47/200)	2.54E-13
Yes tyrosine kinase (YES)	PF07714 ; PF00017 ; PF00018 More >>	23.790 (59/248)	3.65E-13
Erbb4 tyrosine kinase receptor (Erbb-4)	PF00757 ; PF14843 ; PF07714 ; PF01030 More >>	23.881 (48/201)	4.18E-13
Tyrosine-protein kinase Mer (MERTK)	PF00041 ; PF00047 ; PF07714 More >>	26.794 (56/209)	4.26E-13
Inactive tyrosine-protein kinase 7 (PTK7)	PF07679 ; PF13927 ; PF07714 More >>	24.060 (64/266)	4.65E-13
Renal carcinoma antigen NY-REN-64 (IRAK-4)	PF07714	29.032 (63/217)	4.97E-13
Receptor-interacting serine/threonine-protein kinase 2 (RIPK2)	PF00619 ; PF07714	28.571 (76/266)	5.19E-13
Ephrin type-B receptor 1 (EPHB1)	PF14575 ; PF01404 ; PF00041 ; PF07714 ; PF00536 More >>	23.137 (59/255)	5.71E-13
Ankyrin repeat and protein kinase domain-containing protein 1 (ANKK1)	PF12796 ; PF13637 ; PF07714 More >>	27.941 (57/204)	6.30E-13
NT-3 growth factor receptor (TrkC)	PF07679 ; PF00047 ; PF13855 ; PF16920 ; PF01462 ; PF07714 More >>	24.476 (70/286)	6.35E-13
Fibroblast growth factor receptor 2 (FGFR2)	PF07679 ; PF13927 ; PF07714 More >>	20.847 (64/307)	6.67E-13
Tyrosine-protein kinase Srms (SRMS)	PF07714 ; PF00017	25.200 (63/250)	6.88E-13
Vascular endothelial growth factor receptor 2 (KDR)	PF07679 ; PF13927 ; PF07714 ; PF17988 More >>	28.205 (55/195)	1.18E-12
LCK tyrosine protein kinase (LCK)	PF07714 ; PF00017 ; PF00018 More >>	23.438 (60/256)	1.43E-12
Kinase suppressor of Ras-1 (KSR1)	PF07714 ; PF13543 ; PF20406 More >>	23.048 (62/269)	1.57E-12
Activated CDC42 kinase 1 (ACK-1)	PF09027 ; PF11555 ; PF07714 ; PF14604 More >>	24.088 (66/274)	2.40E-12
Ephrin type-A receptor 6 (EPHA6)	PF14575 ; PF01404 ; PF07699 ; PF00041 ; PF07714 ; PF00536 More >>	26.829 (55/205)	2.42E-12
Tyrosine-protein kinase SYK (SYK)	PF07714 ; PF00017	26.500 (53/200)	3.67E-12
Discoidin domain-containing receptor 2 (DDR2)	PF00754 ; PF07714	26.728 (58/217)	4.66E-12
Ephrin type-B receptor 4 (EPHB4)	PF14575 ; PF01404 ; PF07699 ; PF00041 ; PF07714 ; PF00536 More >>	23.438 (60/256)	5.40E-12
Macrophage-stimulating protein receptor (RON)	PF07714 ; PF01403 ; PF01833 More >>	24.031 (62/258)	8.40E-12
Ephrin type-B receptor 3 (EPHB3)	PF14575 ; PF01404 ; PF07699 ; PF00041 ; PF07714 ; PF00536 More >>	22.957 (59/257)	8.84E-12
Muscle-specific kinase receptor (MUSK)	PF01392 ; PF07679 ; PF13927 ; PF07714 More >>	25.430 (74/291)	9.50E-12
Tyrosine-protein kinase UFO (AXL)	PF00041 ; PF13927 ; PF07714 More >>	24.402 (51/209)	1.15E-11
Insulin receptor (INSR)	PF00757 ; PF17870 ; PF07714 ; PF01030 More >>	22.368 (68/304)	1.93E-11
Serine/threonine-protein kinase LMTK1 (AATK)	PF07714	24.519 (51/208)	2.19E-11
Serine/threonine-protein kinase LMTK3 (LMTK3)	PF07714	27.230 (58/213)	2.36E-11
Insulin-like growth factor I receptor (IGF1R)	PF00757 ; PF07714 ; PF01030 More >>	22.878 (62/271)	2.51E-11
Fibroblast growth factor receptor 3 (FGFR3)	PF07679 ; PF13927 ; PF07714 More >>	23.364 (50/214)	4.05E-11
Proto-oncogene c-Met (MET)	PF07714 ; PF01437 ; PF01403 ; PF01833 More >>	26.000 (52/200)	4.17E-11
Dual serine/threonine and tyrosine protein kinase (DSTYK)	PF07714	26.692 (71/266)	5.33E-11
Proto-oncogene c-Fer (FER)	PF00611 ; PF07714 ; PF00017 More >>	26.154 (51/195)	7.78E-11
Fibroblast growth factor receptor 1 (FGFR1)	PF07679 ; PF00047 ; PF07714 More >>	24.766 (53/214)	8.26E-11
TYRO3 tyrosine kinase receptor (TYRO3)	PF00041 ; PF07679 ; PF07714 More >>	22.697 (69/304)	9.23E-11
Vascular endothelial growth factor receptor 3 (FLT-4)	PF07679 ; PF13927 ; PF07714 ; PF17988 More >>	27.711 (46/166)	1.55E-10
Activin receptor-like kinase-1 (ACVRL1)	PF07714 ; PF08515	25.000 (54/216)	2.04E-10
Fibroblast growth factor receptor 4 (FGFR4)	PF07679 ; PF13927 ; PF07714 More >>	22.615 (64/283)	3.06E-10
Serine/threonine-protein kinase TNNI3K (TNNI3K)	PF12796 ; PF13637 ; PF07714 More >>	27.805 (57/205)	3.12E-10
Vascular endothelial growth factor receptor 1 (FLT-1)	PF07679 ; PF00047 ; PF13927 ; PF07714 ; PF17988 More >>	25.888 (51/197)	4.96E-10
Ephrin type-A receptor 10 (EPHA10)	PF14575 ; PF01404 ; PF00041 ; PF07714 ; PF00536 More >>	21.600 (54/250)	7.93E-10
Macrophage colony-stimulating factor 1 receptor (CSF1R)	PF00047 ; PF13927 ; PF07714 More >>	26.415 (42/159)	8.04E-10
Leukocyte receptor tyrosine kinase (LTK)	PF12810 ; PF07714	24.519 (51/208)	2.23E-09
Erbb2 tyrosine kinase receptor (HER2)	PF00757 ; PF14843 ; PF07714 ; PF01030 More >>	22.500 (45/200)	2.76E-09
Tyrosine-protein kinase Kit (KIT)	PF00047 ; PF07714	26.974 (41/152)	3.79E-09
Integrin-linked protein kinase 1 (ILK)	PF12796 ; PF07714	19.828 (46/232)	4.75E-09
Platelet-derived growth factor receptor alpha (PDGFRA)	PF07679 ; PF07714	26.582 (42/158)	5.74E-09
ALK tyrosine kinase receptor (ALK)	PF12810 ; PF00629 ; PF07714 More >>	25.481 (53/208)	6.36E-09
Bone morphogenetic protein receptor type-1A (BMPR1A)	PF01064 ; PF07714 ; PF08515 More >>	23.197 (74/319)	6.46E-09
Atrial natriuretic peptide receptor B (NPR2)	PF01094 ; PF00211 ; PF07714 More >>	26.563 (51/192)	7.40E-09
Nuclear receptor-binding protein (NRBP1)	PF07714	25.088 (71/283)	9.10E-09
Mixed lineage kinase domain-like protein (MLKL)	PF07714	25.000 (56/224)	1.06E-08
Proto-oncogene c-Ros (ROS1)	PF00041 ; PF07714	24.820 (69/278)	1.11E-08
Fms-like tyrosine kinase 3 (FLT-3)	PF00047 ; PF07714	28.659 (47/164)	1.61E-08
Guanylyl cyclase C (GUCY2C)	PF00211 ; PF07714	23.577 (58/246)	1.97E-08
Activin receptor-like kinase 2 (ALK-2)	PF01064 ; PF07714 ; PF08515 More >>	24.537 (53/216)	2.37E-08
Insulin receptor-related protein (INSRR)	PF00757 ; PF07714 ; PF01030 More >>	20.446 (55/269)	5.01E-08
Ephrin type-B receptor 6 (EPHB6)	PF14575 ; PF01404 ; PF07699 ; PF00041 ; PF07714 ; PF07647 More >>	22.886 (46/201)	3.70E-07
Bone morphogenetic protein receptor type-1B (BMPR1B)	PF01064 ; PF07714 ; PF08515 More >>	23.288 (51/219)	6.47E-07
Serine/threonine-protein kinase LMTK2 (LMTK2)	PF07714	23.659 (75/317)	8.46E-07
Tyrosine-protein kinase STYK1 (STYK1)	PF07714	23.179 (35/151)	2.40E-06
Atrial natriuretic peptide receptor A (NPR1)	PF01094 ; PF00211 ; PF07714 More >>	24.084 (46/191)	1.13E-05
TGF-beta receptor type II (TGFBR2)	PF08917 ; PF07714	25.926 (56/216)	2.22E-05
Tyrosine-protein kinase RYK (RYK)	PF07714 ; PF02019	21.875 (63/288)	2.49E-05
Activin receptor type-1C (ACVR1C)	PF01064 ; PF07714 ; PF08515 More >>	21.495 (46/214)	3.56E-05
Platelet-derived growth factor receptor beta (PDGFRB)	PF07679 ; PF00047 ; PF13927 ; PF07714 More >>	28.125 (27/96)	6.45E-05
Retinal guanylyl cyclase 2 (GUCY2F)	PF01094 ; PF00211 ; PF07701 ; PF07714 More >>	20.264 (46/227)	2.00E-03
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References

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REF 1

Nek8, a NIMA family kinase member, is overexpressed in primary human breast tumors. Gene. 2004 Mar 17;328:135-42.

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