Target Information
| Target General Information | Top | |||||
|---|---|---|---|---|---|---|
| Target ID |
T25136
(Former ID: TTDR00448)
|
|||||
| Target Name |
Human immunodeficiency virus Ribonuclease H (HIV RNH)
|
|||||
| Synonyms |
Retroviral ribonuclease H; RNase H (600-1159); RNH1
Click to Show/Hide
|
|||||
| Gene Name |
HIV RNH
|
|||||
| Target Type |
Literature-reported target
|
[1] | ||||
| Function |
Endonuclease that specifically degrades the RNA of RNA- DNA hybrids.
Click to Show/Hide
|
|||||
| UniProt ID | ||||||
| EC Number |
EC 2.7.7.49
|
|||||
| Sequence |
PISPIETVPVKLKPGMDGPKVKQWPLTEEKIKALVEICTEMEKEGKISKIGPENPYNTPV
FAIKKKDSTKWRKLVDFRELNKRTQDFWEVQLGIPHPAGLKKKKSVTVLDVGDAYFSVPL DEDFRKYTAFTIPSINNETPGIRYQYNVLPQGWKGSPAIFQSSMTKILEPFKKQNPDIVI YQYMDDLYVGSDLEIGQHRTKIEELRQHLLRWGLTTPDKKHQKEPPFLWMGYELHPDKWT VQPIVLPEKDSWTVNDIQKLVGKLNWASQIYPGIKVRQLCKLLRGTKALTEVIPLTEEAE LELAENREILKEPVHGVYYDPSKDLIAEIQKQGQGQWTYQIYQEPFKNLKTGKYARMRGA HTNDVKQLTEAVQKITTESIVIWGKTPKFKLPIQKETWETWWTEYWQATWIPEWEFVNTP PLVKLWYQLEKEPIVGAETFYVDGAANRETKLGKAGYVTNKGRQKVVPLTNTTNQKTELQ AIYLALQDSGLEVNIVTDSQYALGIIQAQPDKSESELVNQIIEQLIKKEKVYLAWVPAHK GIGGNEQVDKLVSAGIRKIL Click to Show/Hide
|
|||||
| Drug Binding Sites of Target | Top | |||||
|---|---|---|---|---|---|---|
| Ligand Name: 2'-deoxythymidine triphosphate | Ligand Info | |||||
| Structure Description | STRUCTURE OF A CATALYTIC COMPLEX OF HIV-1 REVERSE TRANSCRIPTASE: IMPLICATIONS FOR NUCLEOSIDE ANALOG DRUG RESISTANCE | PDB:1RTD | ||||
| Method | X-ray diffraction | Resolution | 3.20 Å | Mutation | Yes | [2] |
| PDB Sequence |
KISPIETVPV
10 KLKPGMDGPK20 VKQWPLTEEK30 IKALVEICTE40 MEKEGKISKI50 GPENPYNTPV 60 FAIKKKDSTK70 WRKLVDFREL80 NKRTQDFWEV90 QLGIPHPAGL100 KKKKSVTVLD 110 VGDAYFSVPL120 DEDFRKYTAF130 TIPSINNETP140 GIRYQYNVLP150 QGWKGSPAIF 160 QSSMTKILEP170 FRKQNPDIVI180 YQYMDDLYVG190 SDLEIGQHRT200 KIEELRQHLL 210 RWGLTTPDKK220 HQKEPPFLWM230 GYELHPDKWT240 VQPIVLPEKD250 SWTVNDICKL 260 VGKLNWASQI270 YPGIKVRQLC280 KLLRGTKALT290 EVIPLTEEAE300 LELAENREIL 310 KEPVHGVYYD320 PSKDLIAEIQ330 KQGQGQWTYQ340 IYQEPFKNLK350 TGKYARMRGA 360 HTNDVKQLTE370 AVQKITTESI380 VIWGKTPKFK390 LPIQKETWET400 WWTEYWQATW 410 IPEWEFVNTP420 PLVKLWYQLE430 KEPIVGAETF440 YVDGAANRET450 KLGKAGYVTN 460 KGRQKVVPLT470 DTTNQKTQLQ480 AIYLALQDSG490 LEVNIVTDSQ500 YALGIIQAQP 510 DESESELVNQ520 IIEQLIKKEK530 VYLAWVPAHK540 GIGGNEQVDK550 LVSA |
|||||
|
|
||||||
| Ligand Name: (2-Acetyl-5-methylanilino)(2,6-dibromophenyl)acetamide | Ligand Info | |||||
| Structure Description | STRUCTURE OF HIV-1 REVERSE TRANSCRIPTASE IN A COMPLEX WITH THE NONNUCLEOSIDE INHIBITOR ALPHA-APA R 95845 AT 2.8 ANGSTROMS RESOLUTION | PDB:1HNI | ||||
| Method | X-ray diffraction | Resolution | 2.80 Å | Mutation | No | [3] |
| PDB Sequence |
PISPIETVPV
10 KLKPGMDGPK20 VKQWPLTEEK30 IKALVEICTE40 MEKEGKISKI50 GPENPYNTPV 60 FAIKKKDSTK70 WRKLVDFREL80 NKRTQDFWEV90 QLGIPHPAGL100 KKKKSVTVLD 110 VGDAYFSVPL120 DEDFRKYTAF130 TIPSINNETP140 GIRYQYNVLP150 QGWKGSPAIF 160 QSSMTKILEP170 FKKQNPDIVI180 YQYMDDLYVG190 SDLEIGQHRT200 KIEELRQHLL 210 RWGLTTPDKK220 HQKEPPFLWM230 GYELHPDKWT240 VQPIVLPEKD250 SWTVNDIQKL 260 VGKLNWASQI270 YPGIKVRQLS280 KLLRGTKALT290 EVIPLTEEAE300 LELAENREIL 310 KEPVHGVYYD320 PSKDLIAEIQ330 KQGQGQWTYQ340 IYQEPFKNLK350 TGKYARMRGA 360 HTNDVKQLTE370 AVQKITTESI380 VIWGKTPKFK390 LPIQKETWET400 WWTEYWQATW 410 IPEWEFVNTP420 PLVKLWYQLE430 KEPIVGAETF440 YVDGAANRET450 KLGKAGYVTN 460 KGRQKVVPLT470 NTTNQKTELQ480 AIYLALQDSG490 LEVNIVTDSQ500 YALGIIQAQP 510 DKSESELVNQ520 IIEQLIKKEK530 VYLAWVPAHK540 GIGGNEQVDK550 LVSAGIRK |
|||||
|
|
||||||
| Click to View More Binding Site Information of This Target with Different Ligands | ||||||
| Different Human System Profiles of Target | Top |
|---|---|
|
Human Similarity Proteins
of target is determined by comparing the sequence similarity of all human proteins with the target based on BLAST. The similarity proteins for a target are defined as the proteins with E-value < 0.005 and outside the protein families of the target.
A target that has fewer human similarity proteins outside its family is commonly regarded to possess a greater capacity to avoid undesired interactions and thus increase the possibility of finding successful drugs
(Brief Bioinform, 21: 649-662, 2020).
Human Similarity Proteins
|
|
| Protein Name | Pfam ID | Percentage of Identity (%) | E value |
|---|---|---|---|
| Endogenous retrovirus group K member 9 Pol protein (ERVK-9) | 41.447 (63/152) | 6.97E-28 |
| Target-Related Models and Studies | Top | |||||
|---|---|---|---|---|---|---|
| Target Validation | ||||||
| References | Top | |||||
|---|---|---|---|---|---|---|
| REF 1 | RNase H active site inhibitors of human immunodeficiency virus type 1 reverse transcriptase: design, biochemical activity, and structural information. J Med Chem. 2009 Oct 8;52(19):5781-4. | |||||
| REF 2 | Structure of a covalently trapped catalytic complex of HIV-1 reverse transcriptase: implications for drug resistance. Science. 1998 Nov 27;282(5394):1669-75. | |||||
| REF 3 | Structure of HIV-1 reverse transcriptase in a complex with the non-nucleoside inhibitor alpha-APA R 95845 at 2.8 A resolution. Structure. 1995 Apr 15;3(4):365-79. | |||||
If You Find Any Error in Data or Bug in Web Service, Please Kindly Report It to Dr. Zhou and Dr. Zhang.